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1.
Dev Med Child Neurol ; 59(8): 796-805, 2017 08.
Article in English | MEDLINE | ID: mdl-28439890

ABSTRACT

AIM: To conduct a systematic literature review on patients with biphasic disease with herpes simplex virus (HSV) encephalitis followed by anti-N-methyl-D-aspartate receptor (NMDAR) encephalitis. METHOD: We conducted a case report and systematic literature review (up to 10 December 2016), focused on differences between herpes simplex encephalitis (HSE) and anti-NMDAR encephalitis phases, age-related characteristics of HSV-induced anti-NMDAR encephalitis, and therapy. For statistical analyses, McNemar's test, Fisher's test, and Wilcoxon rank sum test were used (two-tailed significance level set at 5%). RESULTS: Forty-three patients with biphasic disease were identified (31 children). Latency between HSE and anti-NMDAR encephalitis was significantly shorter in children than adults (median 24 vs 40.5d; p=0.006). Compared with HSE, anti-NMDAR encephalitis was characterized by significantly higher frequency of movement disorder (2.5% vs 75% respectively; p<0.001), and significantly lower rate of seizures (70% vs 30% respectively; p=0.001). Compared with adults, during anti-NMDAR encephalitis children had significantly more movement disorders (86.7% children vs 40% adults; p=0.006), fewer psychiatric symptoms (41.9% children vs 90.0% adults; p=0.025), and a slightly higher median modified Rankin Scale score (5 in children vs 4 in adults; p=0.015). During anti-NMDAR encephalitis, 84.6 per cent of patients received aciclovir (for ≤7d in 22.7%; long-term antivirals in 18.0% only), and 92.7 per cent immune therapy, but none had recurrence of HSE clinically or using cerebrospinal fluid HSV polymerase chain reaction (median follow-up 7mo). INTERPRETATION: Our review suggests that movement disorder may help differentiate clinically an episode of HSV-induced anti-NMDAR encephalitis from HSE relapse. Compared with adults, children have shorter latency between HSE and anti-NMDAR encephalitis and, during anti-NMDAR encephalitis, more movement disorder, fewer psychiatric symptoms, and slightly more severe disease. According to our results, immune therapy given for HSV-induced anti-NMDAR encephalitis does not predispose patients to HSE recurrence.


Subject(s)
Anti-N-Methyl-D-Aspartate Receptor Encephalitis/physiopathology , Encephalitis, Herpes Simplex/physiopathology , Mental Disorders/physiopathology , Movement Disorders/physiopathology , Simplexvirus/pathogenicity , Adult , Anti-N-Methyl-D-Aspartate Receptor Encephalitis/complications , Anti-N-Methyl-D-Aspartate Receptor Encephalitis/etiology , Anti-N-Methyl-D-Aspartate Receptor Encephalitis/microbiology , Child , Encephalitis, Herpes Simplex/complications , Female , Humans , Mental Disorders/etiology , Movement Disorders/etiology
2.
PLoS One ; 9(5): e97592, 2014.
Article in English | MEDLINE | ID: mdl-24831942

ABSTRACT

Adrenomedullin (AM) is a multifunctional peptide endowed with various biological actions mediated by the interaction with the calcitonin receptor-like receptor (CLR), which couples to the receptor activity-modifying proteins 2 or 3 (RAMP2 or RAMP3) to form the functional plasma membrane receptors AM1 and AM2, respectively. In this study, we investigated for the first time the expression and localization of AM, CLR, RAMP2 and RAMP3 in human thymic tissue from newborns and in primary cultures of thymic epithelial cells (TECs) and thymocytes. Immunohistochemical analysis of thymic tissue showed that both AM and RAMP2 are abundantly expressed in the epithelial cells of medulla and cortex, blood vessels and mastocytes. In contrast, RAMP3 could not be detected. In cultured TECs, double immunofluorescence coupled to confocal microscopy revealed that AM is present in the cytoplasmic compartment, whereas RAMP2 could be detected in the cytoplasm and nucleus, but not in the cell membrane. At variance with RAMP2, CLR was not only present in the nucleus and cytoplasm of TECs, but could also be detected in the cell membrane. The nuclear and cytoplasmic localizations of RAMP2 and CLR and the absence of RAMP2 in the cell membrane were confirmed by western-blot analysis performed on cell fractions. AM, RAMP2 and CLR could also be detected in thymocytes by means of double immunofluorescence coupled to confocal microscopy, although these proteins were not present in the whole thymocyte population. In these cells, AM and RAMP2 were detected in the cytoplasm, whereas CLR could be observed in the cytoplasm and the plasma membrane. In conclusion, our results show that the AM system is widely expressed in human thymus from newborns and suggest that both AM1 receptor components CLR and RAMP2 are not associated with the plasma membrane of TECs and thymocytes but are located intracellularly, notably in the nucleus.


Subject(s)
Adrenomedullin/metabolism , Gene Expression Regulation , Thymus Gland/growth & development , Calcitonin Receptor-Like Protein/metabolism , Cell Membrane/metabolism , Cell Nucleus/metabolism , Cells, Cultured , Cyclic AMP/metabolism , Cytoplasm/metabolism , Epithelial Cells/cytology , Humans , Immunohistochemistry , Infant, Newborn , Mast Cells/cytology , Microscopy, Fluorescence , Protein Binding , Receptor Activity-Modifying Protein 2/metabolism , Receptor Activity-Modifying Protein 3/metabolism , Thymocytes/cytology , Thymus Gland/metabolism
3.
J Pediatr ; 164(6): 1475-80.e2, 2014 Jun.
Article in English | MEDLINE | ID: mdl-24657119

ABSTRACT

OBJECTIVE: To investigate the clinical manifestations at diagnosis and during follow-up in patients with 22q11.2 deletion syndrome to better define the natural history of the disease. STUDY DESIGN: A retrospective and prospective multicenter study was conducted with 228 patients in the context of the Italian Network for Primary Immunodeficiencies. Clinical diagnosis was confirmed by cytogenetic or molecular analysis. RESULTS: The cohort consisted of 112 males and 116 females; median age at diagnosis was 4 months (range 0 to 36 years 10 months). The diagnosis was made before 2 years of age in 71% of patients, predominantly related to the presence of heart anomalies and neonatal hypocalcemia. In patients diagnosed after 2 years of age, clinical features such as speech and language impairment, developmental delay, minor cardiac defects, recurrent infections, and facial features were the main elements leading to diagnosis. During follow-up (available for 172 patients), the frequency of autoimmune manifestations (P = .015) and speech disorders (P = .002) increased. After a median follow-up of 43 months, the survival probability was 0.92 at 15 years from diagnosis. CONCLUSIONS: Our data show a delay in the diagnosis of 22q11.2 deletion syndrome with noncardiac symptoms. This study provides guidelines for pediatricians and specialists for early identification of cases that can be confirmed by genetic testing, which would permit the provision of appropriate clinical management.


Subject(s)
Abnormalities, Multiple/diagnosis , Developmental Disabilities/epidemiology , DiGeorge Syndrome/diagnosis , Disease Progression , Monitoring, Physiologic/methods , Abnormalities, Multiple/epidemiology , Adolescent , Adult , Age Factors , Child , Child, Preschool , Chromosomes, Human, Pair 22/genetics , Delayed Diagnosis , Developmental Disabilities/diagnosis , DiGeorge Syndrome/genetics , DiGeorge Syndrome/therapy , Early Diagnosis , Female , Follow-Up Studies , Genetic Testing , Humans , Infant , Infant, Newborn , Male , Prospective Studies , Retrospective Studies , Risk Assessment , Severity of Illness Index , Sex Factors , Time Factors , Young Adult
4.
Pediatr Radiol ; 41(8): 962-70, 2011 Aug.
Article in English | MEDLINE | ID: mdl-21597906

ABSTRACT

BACKGROUND: Congenital cytomegalovirus (CMV) infection can lead to severe neurological sequelae, but a defined brain magnetic resonance (MR) pattern and MR predictors of clinical outcome are still lacking. MATERIALS AND METHODS: Clinical and MR findings of 14 children with symptomatic congenital CMV infection were retrospectively reviewed. RESULTS: Microcephaly, cerebral palsy and epilepsy were found in eight, six and seven patients, respectively (all concomitant in 6); 12 children developed sensory-neural hearing loss (SNHL). At first MRI (mean age 21 months, range 5-54 months), white matter (WM) involvement was not assessable in two children due to incomplete myelination. WM abnormalities were common (11/12 patients); deep WM was predominantly involved in 5/11; the largest WM lesion was in the parietal lobe in 6/11. Anterior temporal lobe abnormalities were found in 13/14. Six children underwent MRI examination after 2 years of life; in this subgroup, WM abnormalities were extensive and confluent (4/6), bilateral and multifocal (1/6) or absent (1/6). Four children showed a progression of myelination. Ventriculomegaly (9/14), migration disorders (6/14 polymicrogyria and 1/14 pachygyria-lissencephaly) and hippocampal dysplasia (6/14) correlated with severe neurological sequelae (p < 0.05, Fisher exact test), while the presence of WM abnormalities (11/12), periventricular cysts (6/14) and cerebellar hypoplasia (4/14) did not predict the outcome. CONCLUSIONS: The spectrum of brain MR abnormalities in symptomatic congenital CMV infection is extremely wide. WM involvement is variable, difficult to evaluate at a very young age and unrelated to clinical outcome, while cortical malformations, ventriculomegaly and hippocampal dysplasia seem to be strong predictors of poor outcome except for SNHL.


Subject(s)
Brain/pathology , Cytomegalovirus Infections/pathology , Magnetic Resonance Imaging/methods , Brain/virology , Cerebral Palsy/complications , Cerebral Palsy/pathology , Child, Preschool , Cytomegalovirus Infections/complications , Cytomegalovirus Infections/congenital , Epilepsy/complications , Epilepsy/pathology , Female , Hearing Loss/complications , Hearing Loss/pathology , Humans , Infant , Male , Retrospective Studies
5.
Am J Med Genet A ; 152A(3): 622-9, 2010 Mar.
Article in English | MEDLINE | ID: mdl-20186794

ABSTRACT

Mendelian susceptibility to mycobacterial disease (MSMD) is a rare primary immunodeficiency associated with clinical disease caused by weakly virulent mycobacterial species. Interferon gamma receptor 1 (IFN-gammaR1) deficiency is a genetic etiology of MSMD. We describe the clinical and genetic features of a 7-year-old Italian boy suffering from MSMD associated with a complex phenotype, including neonatal hyperglycemia, neuromuscular disease, and dysmorphic features. The child also developed necrotizing pneumonia caused by Rhodococcus equi. The child is homozygous for a nonsense mutation in exon 3 of IFNGR1 as a result of paternal uniparental disomy (UPD) of the entire chromosome 6. This is the first reported case of uniparental disomy resulting in a complex phenotype including MSMD.


Subject(s)
Abnormalities, Multiple/genetics , Chromosomes, Human, Pair 6/genetics , Mycobacterium Infections/genetics , Receptors, Interferon/deficiency , Receptors, Interferon/genetics , Uniparental Disomy/genetics , Actinomycetales Infections/genetics , Child , Codon, Nonsense , DNA Mutational Analysis , Exons , Fathers , Female , Homozygote , Humans , Male , Pedigree , Phenotype , Rhodococcus equi , Syndrome , Interferon gamma Receptor
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