Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 2 de 2
Filter
Add more filters










Database
Language
Publication year range
1.
Sci Rep ; 9(1): 3099, 2019 02 28.
Article in English | MEDLINE | ID: mdl-30816203

ABSTRACT

In the renal collecting duct, intercalated cells regulate acid-base balance by effluxing protons through the v-H+-ATPase, and bicarbonate via apical pendrin or the basolateral kidney anion exchanger 1 (kAE1). Additionally, collecting duct cells play an essential role in transepithelial absorption of sodium and chloride. Expression of kAE1 in polarized MDCK I cells was previously shown to decrease trans-epithelial electrical resistance (TEER), suggesting a novel role for kAE1 in paracellular permeability. In our study, we not only confirmed that inducible expression of kAE1 in mIMCD3 cells decreased TEER but we also observed (i) increased epithelial absolute permeability to both sodium and chloride, and (ii) that this effect was dependent on kAE1 activity. Further, kAE1 regulated tight junction properties through the tight junction protein claudin-4, a protein with which it physically interacts and colocalizes. These findings unveil a novel interaction between the junctional protein claudin-4 and the kidney anion exchanger, which may be relevant to ion and/or pH homeostasis.


Subject(s)
Anion Exchange Protein 1, Erythrocyte/physiology , Claudin-4/metabolism , Kidney Tubules, Collecting/cytology , Tight Junctions/metabolism , Animals , Biological Transport , Cell Line , Cell Membrane Permeability , Chlorides/metabolism , Electric Impedance , Kidney/metabolism , Mice , Sodium/metabolism
2.
Am J Physiol Cell Physiol ; 307(3): C296-307, 2014 Aug 01.
Article in English | MEDLINE | ID: mdl-24920676

ABSTRACT

Distal renal tubular acidosis (dRTA) can be caused by mutations in the SLC4A1 gene encoding the anion exchanger 1 (AE1). Both recessive and dominant mutations result in mistrafficking of proteins, preventing them from reaching the basolateral membrane of renal epithelial cells, where their function is needed. In this study, we show that two dRTA mutants are prematurely degraded. Therefore, we investigated the degradation pathway of the kidney AE1 G701D mutant that is retained in the Golgi. Little is known about degradation of nonnative membrane proteins from the Golgi compartments in mammalian cells. We show that the kidney AE1 G701D mutant is polyubiquitylated and degraded by the lysosome and the proteosome. This mutant reaches the plasma membrane, where it is endocytosed and degraded by the lysosome via a mechanism dependent on the peripheral quality control machinery. Furthermore, we show that the function of the mutant is rescued at the cell surface upon inhibition of the lysosome and incubation with a chemical chaperone. We conclude that modulating the peripheral quality control machinery may provide a novel therapeutic option for treatment of patients with dRTA due to a Golgi-retained mutant.


Subject(s)
Acidosis, Renal Tubular/metabolism , Anion Exchange Protein 1, Erythrocyte/metabolism , Golgi Apparatus/metabolism , Kidney/metabolism , Proteolysis , Acidosis, Renal Tubular/genetics , Animals , Anion Exchange Protein 1, Erythrocyte/genetics , Cell Line , Cell Membrane/metabolism , Cycloheximide/pharmacology , Dogs , Endocytosis/physiology , Endoplasmic Reticulum/genetics , Endoplasmic Reticulum/physiology , Endosomal Sorting Complexes Required for Transport/genetics , Endosomal Sorting Complexes Required for Transport/metabolism , Gene Knockdown Techniques , Golgi Apparatus/genetics , HEK293 Cells , HeLa Cells , Humans , Kidney/cytology , Lysosomes , Madin Darby Canine Kidney Cells , Mutation , Protein Synthesis Inhibitors/pharmacology , Protein Transport , Ubiquitination
SELECTION OF CITATIONS
SEARCH DETAIL
...