Corrigendum to 'Amyloid adhesin production in activated sludge is enhanced in lab-scale sequencing batch reactors: Feeding regime impacts microbial community and amyloid distribution' [Water Research X 17 (2022) 100162].

[This corrects the article DOI: 10.1016/j.wroa.2022.100162.].

Ready for the worst? Negative affect in anticipation of a stressor does not protect against affective reactivity.

INTRODUCTION: Lay wisdom suggests feeling negative while awaiting an upcoming stressor-anticipatory negative affect-shields against the blow of the subsequent stressor. However, evidence is mixed, with different lines of research and theory indirectly suggesting that anticipatory negative affect is helpful, harmful, or has no effect on emotional outcomes. In two studies, we aimed to reconcile these competing views by examining the affective trajectory across hours, days, and months, separating affective reactivity and recovery. METHODS: In Study 1, first-year students (N = 101) completed 9 days of experience sampling (10 surveys/day) as they received their first-semester exam grades, and a follow-up survey 5 months later. In Study 2, participants (N = 73) completed 2 days of experience sampling (60 surveys/day) before and after a Trier Social Stress Test. We investigated the association between anticipatory negative affect and the subsequent affective trajectory, investigating (1) reactivity immediately after the stressor, (2) recovery across hours (Study 2) and days (Study 1), and (3) recovery after 5 months (Study 1). RESULTS: Across the two studies, feeling more negative in anticipation of a stressor was either associated with increased negative affective reactivity, or unassociated with affective outcomes. CONCLUSION: These results run counter to the idea that being affectively ready for the worst has psychological benefits, suggesting that instead, anticipatory negative affect can come with affective costs.

Amyloid adhesin production in activated sludge is enhanced in lab-scale sequencing batch reactors: Feeding regime impacts microbial community and amyloid distribution.

Amyloid adhesins are ß-sheet-rich extracellular proteins thought to contribute to bioflocculation. They are present in activated sludge to varying extent. However, it remains unclear which operational conditions promote their production. To this end, the abundance and distribution of amyloids and their potential producers were monitored in two lab-scale reactors operated in sequencing batch mode with an unaerated and aerated reaction phase. Various feeding regimes ranging from feast-famine to nearly continuous feeding were applied. Thioflavin T staining revealed more amyloids in the lab-scale reactors during all operational stages compared to the full-scale industrial and municipal inocula. Furthermore, the feeding regime impacted the distribution of produced amyloids from dense clusters during feast-famine conditions towards a dispersed distribution during nearly continuous feeding. This dispersed presence did not negatively impact the bioflocculation (towards average floc size and shear sensitivity). 16S rRNA sequencing detected several known EPS and amyloid producers. More continuous and, hence, partially aerobic feeding promoted the relative abundance of denitrifiers. Sequential Thioflavin T staining and fluorescence in situ hybridization identified Zoogloea and Ca. Competibacter as potential amyloid producers under the applied conditions. This experiment confirms that amyloid producers need to be triggered for production and that the feeding regime impacts the microbial community composition, which in turn influences the amyloid production and distribution.

Multidimensional feature interactions in visual crowding: When configural cues eliminate the polarity advantage.

Crowding occurs when surrounding objects (flankers) impair target perception. A key property of crowding is the weaker interference when target and flankers strongly differ on a given dimension. For instance, identification of a target letter is usually superior with flankers of opposite versus the same contrast polarity as the target (the "polarity advantage"). High performance when target-flanker similarity is low has been attributed to the ungrouping of target and flankers. Here, we show that configural cues can override the usual advantage of low target-flanker similarity, and strong target-flanker grouping can reduce - instead of exacerbate - crowding. In Experiment 1, observers were presented with line triplets in the periphery and reported the tilt (left or right) of the central line. Target and flankers had the same (uniform condition) or opposite contrast polarity (alternating condition). Flanker configurations were either upright (||), unidirectionally tilted (\\ or //), or bidirectionally tilted (\/ or /\). Upright flankers yielded stronger crowding than unidirectional flankers, and weaker crowding than bidirectional flankers. Importantly, our results revealed a clear interaction between contrast polarity and flanker configuration. Triplets with upright and bidirectional flankers, but not unidirectional flankers, showed the polarity advantage. In Experiments 2 and 3, we showed that emergent features and redundancy masking (i.e. the reduction of the number of perceived items in repeating configurations) made it easier to discriminate between uniform triplets when flanker tilts were unidirectional (but not when bidirectional). We propose that the spatial configurations of uniform triplets with unidirectional flankers provided sufficient task-relevant information to enable a similar performance as with alternating triplets: strong-target flanker grouping alleviated crowding. We suggest that features which modulate crowding strength can interact non-additively, limiting the validity of typical crowding rules to contexts where only single, independent dimensions determine the effects of target-flanker similarity.

Broad attention uncovers benefits of stimulus uniformity in visual crowding.

Crowding is the interference by surrounding objects (flankers) with target perception. Low target-flanker similarity usually yields weaker crowding than high similarity ('similarity rule') with less interference, e.g., by opposite- than same-contrast polarity flankers. The advantage of low target-flanker similarity has typically been shown with attentional selection of a single target object. Here, we investigated the validity of the similarity rule when broadening attention to multiple objects. In three experiments, we measured identification for crowded letters (Experiment 1), tumbling Ts (Experiment 2), and tilted lines (Experiment 3). Stimuli consisted of three items that were uniform or alternating in contrast polarity and were briefly presented at ten degrees eccentricity. Observers reported all items (full report) or only the left, central, or right item (single-item report). In Experiments 1 and 2, consistent with the similarity rule, single central item performance was superior with opposite- compared to same-contrast polarity flankers. With full report, the similarity rule was inverted: performance was better for uniform compared to alternating stimuli. In Experiment 3, contrast polarity did not affect performance. We demonstrated a reversal of the similarity rule under broadened attention, suggesting that stimulus uniformity benefits crowded object recognition when intentionally directing attention towards all stimulus elements. We propose that key properties of crowding have only limited validity as they may require a-priori differentiation of target and context.

mobileQ: A free user-friendly application for collecting experience sampling data.

In this article we introduce mobileQ, which is a free, open-source platform that our lab has developed to use in experience sampling studies. Experience sampling has several strengths and is becoming more widely conducted, but there are few free software options. To address this gap, mobileQ has freely available servers, a web interface, and an Android app. To reduce the barrier to entry, it requires no high-level programming and uses an easy, point-and-click interface. It is designed to be used on dedicated research phones, allowing for experimenter control and eliminating selection bias. In this article, we introduce setting up a study in mobileQ, outline the set of help resources available for new users, and highlight the success with which mobileQ has been used in our lab.

Disrupting uniformity: Feature contrasts that reduce crowding interfere with peripheral word recognition.

Peripheral word recognition is impaired by crowding, the harmful influence of surrounding objects (flankers) on target identification. Crowding is usually weaker when the target and the flankers differ (for example in color). Here, we investigated whether reducing crowding at syllable boundaries improved peripheral word recognition. In Experiment 1, a target letter was flanked by single letters to the left and right and presented at 8° in the lower visual field. Target and flankers were either the same or different in regard to contrast polarity, color, luminance, and combined color/luminance. Crowding was reduced when the target differed from the flankers in contrast polarity, but not in any of the other conditions. Using the same color and luminance values as in Experiment 1, we measured recognition performance (speed and accuracy) for uniform (e.g., all letters black), congruent (e.g., alternating black and white syllables), and incongruent (e.g., alternating black and white non-syllables) words in Experiment 2. Participants verbally reported the target word, briefly displayed at 8° in the lower visual field. Congruent and incongruent words were recognized slower compared to uniform words in the opposite contrast polarity condition, but not in the other conditions. Our results show that the same feature contrast between the target and the flankers that yielded reduced crowding, deteriorated peripheral word recognition when applied to syllables and non-syllabic word parts. We suggest that a potential advantage of reduced crowding at syllable boundaries in word recognition is counteracted by the disruption of word uniformity.

Inoculation history affects community composition in experimental freshwater bacterioplankton communities.

Priority effects occur when the arrival order of species or genotypes has a lasting effect on community or population structure. For freshwater bacteria, priority effects have been shown experimentally among individual species, but no experiments have been performed using complex natural communities. We investigated experimentally whether a foreign bacterioplankton community influences the community assembly trajectory when inoculated prior to the local community, whether inoculation time lag affects priority effects, and how the individual OTUs responded to time lag. Two bacterioplankton communities from dissimilar ponds were inoculated into one of the natural media with a time lag of 0, 12, 36 or 60 h, giving advantage in time to the foreign community. All three time lags resulted in priority effects, as the final community composition of these treatments differed significantly from that of the treatment with no time lag, but compositional shifts were not linear to inoculation time lag. The responses of individual OTUs to time lag were highly diverse and not predictable based on their immigration history or relative abundance in the inocula or control. The observed impact and complexity of priority effects in multispecies systems emphasize the importance of this process in structuring both natural and industrial bacterial communities.