Prognostic Value and Immune Infiltration of Anoikis-related LncRNAs in Lung Adenocarcinoma / 肿瘤防治研究

Objective To explore the prognostic value and immune infiltration landscape of anoikis-related long noncoding RNAs (arlncRNAs) in lung adenocarcinoma. Methods RNA-seq and clinical data of lung adenocarcinoma were downloaded from the TCGA database, and anoikis-related genes were obtained from the GeneCards and Harmonizome databases. Coexpression, differential, and WGCNA analyses were performed to screen differentially expressed arlncRNAs closely related to the occurrence of lung adenocarcinoma. A prognostic risk model was then constructed based on the arlncRNAs, and its predictive efficacy was further validated. Finally, consensus clustering was used to identify the molecular subtypes associated with anoikis in lung adenocarcinoma. Results Seven prognostic arlncRNAs were identified, and the prognostic risk models established based on them had AUC values of ROC curves greater than 0.7. Survival and immune infiltration analyses revealed that low-risk patients had high overall survival and immune infiltration, implying that they experienced good immune treatment effects. Drug sensitivity analysis showed that the high-risk patients were more sensitive to commonly used chemotherapeutic agents than the low-risk patients. According to the expression of model genes, subtypes C1 and C2 were identified through consensus clustering, and C1 showed a good prognosis. Conclusion The prognostic risk model based on the seven arlncRNAs can effectively predict the prognosis of lung adenocarcinoma patients. The results of immune-related and drug sensitivity analyses provide a reference for the precise individualized treatment of patients with lung adenocarcinoma.

The mTOR/ULK1 signaling pathway mediates the autophagy-promoting and osteogenic effects of dicalcium silicate nanoparticles.

A novel bioactive inorganic material containing silicon, calcium and oxygen, calcium silicate (Ca2SiO4, C2S) with a CaO-SiO2 ingredient, has been identified as a potential candidate for artificial bone. Autophagy has an essential function in adult tissue homoeostasis and tumorigenesis. However, little is known about whether silicate nanoparticles (C2S NPs) promote osteoblastic differentiation by inducing autophagy. Here we investigated the effects of C2S NPs on bone marrow mesenchymal stem cell differentiation (BMSCs) in osteoblasts. Furthermore, we identified the osteogenic gene and protein expression in BMSCs treated with C2S NPs. We found that autophagy is important for the ability of C2S NPs to induce osteoblastic differentiation of BMSCs. Our results showed that treatment with C2S NPs upregulated the expression of BMP2, UNX2, and OSX in BMSCs, and significantly promoted the expression of LC3 and Beclin, while P62 (an autophagy substrate) was downregulated. C2S NP treatment could also enhance Alizarin red S dye (ARS), although alkaline phosphatase (ALP) activity was not significantly changed. However, all these effects could be partially reversed by 3-MA. We then detected potential signaling pathways involved in this biological effect and found that C2S NPs could activate autophagy by suppressing mTOR and facilitating ULK1 expression. Autophagy further activated ß-catenin expression and promoted osteogenic differentiation. In conclusion, C2S NPs promote bone formation and osteogenic differentiation in BMSCs by activating autophagy. They achieve this effect by activating mTOR/ULK1, inducing autophagy, and subsequently triggering the WNT/ß-catenin pathway to boost the differentiation and biomineralization of osteoblasts.

Maternal dietary and environmental risk factors in children with asthma comorbid neuropsychiatric disorders / 中华实用儿科临床杂志

Objective@#To explore the prenatal dietary and environmental risk factors in children aged 6-16 years with asthma (CWA) comorbid neuropsychiatric disorders.@*Methods@#This cross-sectional study was conducted from March 2016 to March 2017.CWA aged 6 to 16 years were recruited as the study participants at the Outpatient Asthma Unit of Guizhou Provincial People′s Hospital, China.Informed consent was obtained from their parents, and their parents were invited to participate in the Mini International Neuropsychiatric Interview of children and adolescents (MINI Kid) to assess the current neuropsychiatric disorders.Meanwhile, the questionnaires information about prenatal diet and environment were collected from their mothers as well.Patients were divided into 2 groups, one group with asthma comorbid neuropsychiatric disorders and the other with asthma only.Single factor analysis and multivariate non-conditional Logistic regression analysis were used to determine the risk factors associated with neuropsychiatric comorbidity.@*Results@#The study consisted of 261 patients (male: 163 cases, female: 98 cases) with asthma, with the age of (11.25±3.12) years, the rate of asthma comorbid neuropsychiatric disorders was 26.4% (69/261 cases). Single factor analysis showed that prenatal tobacco smoke exposure, winter coal combustion, food additives had statistically significant differences between the 2 group (χ2=7.385, 3.993, 4.529, all P<0.05), the other factors were not significantly different between two groups (all P>0.05); multivariate analysis indicated that prenatal tobacco smoke exposure was the independent risk factor for neuropsychiatric comorbidity in CWA (OR=5.098, 95%CI: 1.089-23.871, P=0.039).@*Conclusions@#The result revealed that the prenatal tobacco smoke exposure is the independent risk factor for neuropsychiatric comorbidity in CWA, need to enhance the propaganda and education of negative effects for parents should be enhanced during pregnancy to diminish the prevalence.

Maternal dietary and environmental risk factors in children with asthma comorbid neuropsychiatric disorders / 中华实用儿科临床杂志

Objective To explore the prenatal dietary and environmental risk factors in children aged 6-16 years with asthma (CWA) comorbid neuropsychiatric disorders.Methods This cross-sectional study was conducted from March 2016 to March 2017.CWA aged 6 to 16 years were recruited as the study participants at the Outpatient Asthma Unit of Guizhou Provincial People's Hospital,China.Informed consent was obtained from their parents,and their parents were invited to participate in the Mini International Neuropsychiatric Interview of children and adolescents (MINI Kid) to assess the current neuropsychiatric disorders.Meanwhile,the questionnaires information about prenatal diet and environment were collected from their mothers as well.Patients were divided into 2 groups,one group with asthma comorbid neuropsychiatric disorders and the other with asthma only.Single factor analysis and multivariate non-conditional Logistic regression analysis were used to determine the risk factors associated with neuropsychiatric comorbidity.Results The study consisted of 261 patients (male:163 cases,female:98 cases) with asthma,with the age of (11.25 _± 3.12) years,the rate of asthma comorbid neuropsychiatric disorders was 26.4％ (69/261 cases).Single factor analysis showed that prenatal tobacco smoke exposure,winter coal combustion,food additives had statistically significant differences between the 2 group (x2 =7.385,3.993,4.529,all P ＜ 0.05),the other factors were not significantly different between two groups (all P ＞ 0.05);multivariate analysis indicated that prenatal tobacco smoke exposure was the independent risk factor for neuropsychiatric comorbidity in CWA (OR =5.098,95％ CI:1.089-23.871,P =0.039).Conclusions The result revealed that the prenatal tobacco smoke exposure is the independent risk factor for neuropsychiatric comorbidity in CWA,need to enhance the propaganda and education of negative effects for parents should be enhanced during pregnancy to diminish the prevalence.

Steroids from Monascus purpureus metabolite / 中国中药杂志

<p><b>OBJECTIVE</b>To study the chemical constituents of Monascus purpureus metabolite.</p><p><b>METHOD</b>The compounds were isolated by column chromatography methods, and their structures were determined by spectroscopic methods.</p><p><b>RESULT</b>Eight compounds were isolated from the petroleum ether fraction of ethanolic extract and elucidated as stigmast-4-en-3-one (1), 3-oxo-24-methylenecycloarane (2), stigmasterol (3), 7beta-hydroxystigmasterol (4), 3beta-hydroxystigmast-5-en-7-one (5), 3beta-hydroxystigmast-5,22-dien-7-one (6), 5alpha, 8alpha-epidioxyergosta-6,22-dien-3beta-ol (7), sitosterol (8).</p><p><b>CONCLUSION</b>All of the compounds were isolated from this genu for the first time except compound 3 and 7.</p>