Pityrosporum ovale/orbiculare in papillomatous nevus cell nevi.

62% of 200 papillomatous nevus cell nevi compared to 45% of 40 seborrheic keratoses showed the presence of Pityrosporum ovale/orbiculare (Malassezia furfur) on their surface, supposedly representing a saprophytic state of the microorganisms in vivo. The papillomatous nevus cell nevi were of the compound or dermal type. Significant inflammation, dysplasia or major changes in color were absent. Electron microscopy revealed the characteristic, sometimes budding fungal cells. A relationship between the presence of Pityrosporum organisms and papillomatosis is discussed.

[Panniculitis with liquefaction--a special manifestation of Pfeiffer-Weber-Christian panniculitis]. / Pannikulitis mit Liquefaktion--eine besondere Verlaufsform der Pfeiffer-Weber-Christian-Pannikulitis.

A case of nodular panniculitis with liquefaction in a 58-year-old woman patient is reported. It seems that Pfeiffer-Weber-Christian disease and nodular panniculitis with liquefaction are varying expressions of the same disease entity. The relationship to cases reported in the literature is discussed.

[Argyria. A clinical, chemical analytic and micromorphologic study]. / Zur Frage der Argyrie. Eine klinische, analytisch-chemische und mikromorphologische Untersuchung.

A 62-year-old male patient developed generalized argyria following the intake of silver-proteinacetyltannate (Targesin; approx. 60 g in 10 years) as treatment for gastric discomfort. On histological and ultrastructural examination of the skin, silver particles were found not only in the usual locations but also in the Schwann cell, the mast cell, and in smooth muscle cells. This corresponded to chemical analysis, proving the presence of this metal in the skin. In the blood, a level of 0.26 +/- 0.04 ppm silver was found. By means of an equation, attempts were made to demonstrate the reaction process involved in the formation of Ag2S as subjected to the photochemical effect of sunlight.

[Erythrodermic sarcoidosis (a clinical, light and electron microscopy study)]. / Zur Frage der erythrodermischen Sarkoidose (Eine klinische, licht- und elektronenmikroskopische Untersuchung).

We report on a 50-year-old patient suffering from erythrodermic sarcoidosis accompanied by visible enlargement of the peripheric lymph nodes. Histologic examination revealed epithelioid granulomas in the skin, in a lymph node, and in a liver biopsy. Ultrastructurally, we found a focal presence of cells with Langerhans' granules sometimes in a spatial relation to lymphoid cells within the corial granulomas.

[Pseudoangiosarcoma (Masson). A histological study (author's transl)]. / Pseudoangiosarkom (Masson). Eine histologische Untersuchung.

The following is a report about two cases of pseudoangiosarcoma (Masson). The pseudoangiosarcomatous changes developed in one case in a cavernous hemangioma and in the other on the heel, presumably in the lumen of a vessel of the dermis. In our opinion thrombosis precedes the pseudoangiosarcomatous proliferation, which can be interpreted as a manifestation of a peculiar form of the organization of thrombotic material. Another possibility is discussed namely that the papillary endothelial proliferation is the earliest event in the formation of pseudoangiosarcoma.

[Ultrastructure of adipose cells]. / Zur Ultrastruktur der Fettzellen.

This report presents an ultrastructural study of normal subcutaneous fatty tissue. From well-know morphological facts on human adipocytes in adults we have been able to make the following additional observations: (a) Several lipid droplets were surrounded by a well-developed system of filaments; (b) material very similar (the same electron-density) to the central lipid droplet appeared within the smooth surfaced cisterns of the endoplasmatic reticulum; (c) complex lipid granules very probably corresponding to lipofuscin were present in the cytoplasm; (d) in the nuclei, often sphaeridia of the filamentous type appeared; (e) the quantity of so-called lipomicrons (electron-dense granules) is dependent on the method of fixation.

[On the question of cutaneous changes associated with paraproteinemia. Light- and electron-microscopic study (author's transl)]. / Zur Frage der Hautveränderungen bei einer Doppelparaproteinämie. Eine Licht- und elektronenmikroskopische Untersuchung.

This report deals with a case of paraproteinemia (IgG Kappa) associated with skin indurations and yellowish papules on the trunk, edema of the face, and crystals in the cornea. In addition to the absence of elastic fibers and reductions of collagen fibers, light microscopy revealed single and/or multinucleated dermal cells with slight streaky patterning of the cytoplasm. These cells contained some sudanophilic (the greatest quantities of sudanophilic substances were observed in the deep corium) and partly birefringent material. In particular after warming, numerous spherocrystals appeared, which give Maltese cross birefringence. The presence of cholesterol esters can be assumed. The light-microscopic localization of acid phosphatase (incubation in Gomori medium) coincides in part with the distribution of bifringent lipids. Electron microscopy showed the cells with streaky contents to be chiefly histiocytes with numerous cristalline bodies in their cytoplasm. The cristalloids with a periodic inner structure are always surrounded by a single membrane. Identical bodies can be detected in endothelial cells of capillaries and epidermal keratinocytes. After pronase P incubation they disappear completely. We assume that the deposits correspond to phagocytized paraprotein. In addition, some histiocytic cells contain numerous lipid droplets in their cytoplasm. There is disintegration of some cells with crystalline inclusion, particularly in the deep corium. These cells show not only diffuse intracytoplasmic but also extracellular distribution of acid phosphatase activities. Particularly in the pericellular space of such cells numerous "banded structures" appear in connection with collagen degradation probably initiated by lysosomal enzymes. Amyloid could not be detected either light- or electron-microscopically.

[Juvenile melanoma; a clinical and histological study]. / Das juvenile Melanom; Eine klinische und histologische Untersuchung.

Investigations were carried out in 52 female and 18 male patients, aged between 2 and 49 years, with juvenile melanoma. The tumor occured as a solitary tumor in all cases except one. It was mainly localized on the head (54%), and the extremities, on the trunk in three cases only. In 35% the tumor could be diagnosed clinically. Light microscopy revealed in 37.1% spindle cells, in 17.1% epitheloid cells, and in 45.7% the juvenile melanoma was of the mixed type. A proliferation of the vascular endothelial cells (37.1%) as well as immature looking mast cells in the tumor region were prominent findings. A transepidermal elimination of tumor cell nests was noted in one case. The other results are in agreement with earlier descriptions of this tumor.

[The ultrastructure and acid phosphatase activity in the sweat glands with "clear, reticulated cytoplasm" (author's transl)]. / Zur Ultrastruktur und sauren Phosphatase-Aktivität in den Schweissdrüsen mit "clear, reticulated cytoplasm"

Investigations were carried out on the ultrastructure and distribution of acid phosphatase in the human sweat glands with "clear, reticulated cytoplasm". It is probable that the gland cells with vacuolized cytoplasm develop from the serous gland cells through a morphologically recognizable intermediate state. An extralysosomal activity of acid phosphatase occurs in the vacuolized gland cells. The free distribution of the enzyme activity is focal. A correlation between these findings and a possible holocrine mode of secretion is discussed.

[Histochemical and ultrastructural study of cytochrome-oxydase in basal cell epithelioma]. / Etude histochimique et ultrastructurale de la cytochrome-oxydase dans l'épithélioma baso-cellulaire

The activity of cytochrome-oxydase is less pronounced in the basal cell epithelioma than in the basal cells of the surrounding normal epidermis. The electron microscopic study of the localisation of the enzyme reveals that the cells of the basal cell epithelioma contain two types of mitochondria, the first of which having the same size as the epidermal mitochondria, and the other being giant mitochondria. The latter seem to be less active. The weak reactivity of the basal cell epithelioma for cytochrome-oxydase is probably due to the reduction of the cristae in mitochondria and to the functional deficiency of the giant mitochondria.

On the occurrence of sphaeridia in basalioma cells and the basal cells of the overlying epidermis.

302 nuclear sections of basalioma cells, 145 nuclear sections of the basal cells of the epidermis above the tumor, and 177 nuclear sections of the basal cells of normal epidermis were investigated and statistically evaluated with the aim of determining the presence of sphaeridia occur significantly more frequently in the basal cells of the epidermis above the tumor and in normal epidermis than in the tumor itself. These findings are discussed in connection with the degree of cellular protein synthesis.

[Hydrotherapy in cauterization wounds. A histologic study (author's transl)]. / Zur Frage der Hydrotherapie bei Verätzungen. Eine histologische Untersuchung

Investigations have have been carried out in guinea pigs to test the usefullness of hydrotherapy. Concentrated sulfuric acid was applied to the skin for a period of 30 sec and 5 min. After this the skin was washed with tap water for 1 hr. The treated area was then subjected to macroscopic and microscopic examination. When the acid was applied for 30 sec, followed by rinsing, an almost complete restitution of the skin was found on the test area macroscopically and microscopically. Significant differences were also observed after an exposition of 5 min in comparison with untreated control animals.