The effect of the microscopic and nanoscale structure on bone fragility.

Bone mineral density is the gold-standard for assessing bone quantity and diagnosing osteoporosis. Although bone mineral density measurements assess the quantity of bone, the quality of the tissue is an important predictor of fragility. Understanding the macro- and nanoscale properties of bone is critical to understanding bone fragility in osteoporosis. Osteoporosis is a disease that affects more than 75 million people worldwide. The gold standard for osteoporosis prognosis, bone mineral density, primarily measures the quantity of bone in the skeleton, overlooking more subtle aspects of bone's properties. Bone quality, a measure of bone's architecture, geometry and material properties, is evaluated via mechanical, structural and chemical testing. Although decreased BMD indicates tissue fragility at the clinical level, changes in the substructure of bone can help indicate how bone quality is altered in osteoporosis. Additionally, mechanical properties which can quantify fragility, or bone's inability to resist fracture, can be changed due to alterations in bone architecture and composition. Recent studies have focused on examination of bone on the nanoscale, suggesting the importance of understanding the interactions of the mineral crystals and collagen fibrils and how they can alter bone quality. It is therefore important to understand alterations in bone that occur at the macro-, micro- and nanoscopic levels to determine what parameters contribute to decreased bone quality in diseased tissue.

In vitro dentinal surface reaction of 9.5% buffered hydrofluoric acid in repair of ceramic restorations: a scanning electron microscopic investigation.

STATEMENT OF PROBLEM: Fracture of porcelain is a relatively common clinical misfortune. Recent research has indicated that strong bonds can be formed between composite and dental porcelain. Porcelain surfaces are etched with hydrofluoric acid and treated with silane coupling agents before composite application. The question is how exposed dentin may react to etching with hydrofluoric acid. PURPOSE: This investigation examined the effect of 9.5% buffered hydrofluoric acid, of 36% o-phosphoric acid alone and in combination on the surface structure of cut human dentin. MATERIAL AND METHODS: Human molar teeth were sectioned in approximately 0.8-mm thick slices and treated with different acids or their combinations. Application periods were 10, 60, and 180 seconds. Specimens were processed for SEM and for energy-dispersive x-ray (EDX) microanalysis. RESULTS: The smear layer on the surface of sectioned dentin was not completely removed by hydrofluoric acid alone and that a dense amorphous precipitate was formed on the peritubular zone. Starlike structures in dentinal tubules were visible. EDX analysis revealed different fluoride content on the treated surface, dependent on the etchant used. CONCLUSION: Topical application of hydrofluoric acid appeared to provide a dentinal surface with an amorphous precipitate of fluoride. This layer may be important both for resistance of dental caries in dentin and for bonding reactions.

Indole glucosinolate and auxin biosynthesis in Arabidopsis thaliana (L.) Heynh. glucosinolate mutants and the development of clubroot disease.

Mutants and wild type plants of Arabidopsis thaliana were analysed for differences in glucosinolate accumulation patterns, indole-3-acetic acid (IAA) biosynthesis and phenotype. A previously identified series of mutants, termed TU, with altered glucosinolate patterns was used in this study. Only the line TU8 was affected in shoot phenotype (shorter stems, altered branching pattern). Synthesis of IAA and metabolism were not much affected in the TU8 mutant during seedling development, although the content of free IAA peaked earlier in TU8 during plant development than in the wild type. Indole glucosinolates and IAA may, however, be involved in the development of clubroot disease caused by the obligate biotrophic fungus Plasmodiophora brassicae since the TU3 line had a lower infection rate than the wild type, and lines TU3 and TU8 showed decreased symptom development. The decline in clubroot formation was accompanied by a reduced number of fungal structures within the root cortex and slower development of the fungus. Indole glucosinolates were lower in infected roots of TU3 and TU8 than in control roots of these lines, whereas in wild-type plants the differences were not as prominent. Free IAA and indole-3-acetonitrile (IAN) were increased in infected roots of the wild type and mutants with normal clubroot symptoms, whereas they were reduced in infected roots of mutants TU3 and TU8. These results indicate a role for indole glucosinolates and IAN/IAA in relation to symptom development in clubroot disease.

The growth of lung volumes affected by physical performance capacity in boys and girls during childhood and adolescence.

The paper concerns a longitudinal study of the relationship between growth in lung functions in terms of forced vital capacity and forced expiratory volume in 1 s, and the development of maximal aerobic power during the age span from 8-18 years of age. The growth curves of anatomical dimensions for boys and girls were similar to those previously established for Northern European children. The growth in lung volume ended later than the growth in body height. It was found that the growth in lung volume was entirely due to growth in body dimensions, with no additional effect of changes in the development of physical performance capacity.

Leisure time sport activities and maximal aerobic power during late adolescence.

The pattern of leisure time sport activity was estimated by retrospective recalls and expressed in terms of an annual sport activity score. The activity score was related to the development of maximal aerobic power during the period of late adolescence in German children. Both girls and boys reduced their activity pattern from 14 to 18 years of age, boys more than girls. At each age boys were more active than girls. A slight tendency towards better fitness with increased habitual physical activity was noticed, but many sedentary children exhibited a good performance capacity and some children with a high level of leisure time sport activity were characterized by a low level of maximal aerobic power. A statistical analysis revealed that the observed tendency to better fitness with increased habitual physical activity could be explained by an age factor with no additional effect of variation in sport activity score.

Maximal aerobic power affected by maturation and body growth during childhood and adolescence.

This paper examines the development of maximal aerobic power as a function of maturation by a longitudinal study with annual examinations of representative samples of 56 boys and 56 girls in Norway and Western Germany. The age at which occurred peak height velocity (PHV) (i.e. the age at which the greatest height velocity was observed) was used as a reference of biological age and maturation. Before the PHV the mean maximal aerobic power was the same in two cohorts of children both in absolute values as well as in values relative to total and lean body mass. At and after PHV the boys were similar in their absolute values, but the German girls decreased their exercise fitness and became inferior to the Norwegian girls during later adolescence. When related to age of PHV the maximal aerobic power increased during the prepubertal years, mainly as an effect of growth in body size with little or no additional effect of other factors and approached a ceiling level at the end of adolescence. The boys exhibited clearly superior exercise fitness during all years of childhood and adolescence, this being in contrast to the widely accepted concept that no sex difference exists in exercise fitness before puberty. The maximum level for VO2-max, reached at the end of adolescence, averaged 3.2 1/min with a coefficient of variation of about 12% for the boys, and 2.5 1/min for the Norwegian girls and a similar coefficient of variation. As these ceiling values of maximal oxygen uptake agree with published averages for normal young adults representative for the normal population in these two countries, it is suggested that they represent optimal values brought about mainly by normal growth in body size with no or little additional effects of other factors.

Maximum aerobic power and body composition during the puberty growth period: similarities and differences between children of two European countries.

This report gives results of a longitudinal study of two cohorts of school children in Norway and West-Germany. The rate of growth in body size and composition is identical for the two samples, but different for the two sexes, and follows closely the trend of growth which has been found for North-Europeans in general. Despite of this similarity in growth of anatomical variables the Norwegian children appeared to be superior in their maximum aerobic power at all comparable ages and in both sexes. The differences between means in maximal oxygen uptake varies somewhat with age and sex and are in the range of 5-10%. It is suggested that the mean differences between Norwegian and German children in their exercise and cardio-vascular fitness are brought about by a more physically active behavioural pattern of living in Norway.

Excercise ventilation during the growth spurt period: comparison between two European countries.

The paper provides reference values with regard to pulmonary ventilation responses o progressively increasing bicycling up to the maximal level during the period of growth spurt. Data are based on longitudinal studies or rural Norwegian and German school children. A cluster sampling technique was used, starting with the total pupil-population at Lom in Norway at an age of 8 years, and annual tests were performed until the age of 15 years. In germany a similar pupil-population was tested from age 12 until age 17 years. Functional growth curves giving means and dispersions for ventilation rates, tidal volume, respiratory frequency, ventilatory equivalent and respiratory gas exchange ratio are constructed. Norwegian and German children's developmental processes with regard to the exercise ventilation variables were compared, and revealed no significant differences.

Gastric pathology produced by hypothalamic lesions in rats.

Rats made aphagic and adipsic by damage to the lateral hypothalamus develop gastric lesions and lose body weight excessively over a four day period. Control observations indicate that these effects cannot be accounted for by food and water deprivation or by operative trauma. The possibility that gastric lesions may contribute to aphagia or anorexia is discussed.