ABSTRACT
Trichosporonosis due to Trichosporon beigelii is increasingly recognized in neutropenic immunocompromised patients. We report the clinical and histological presentation of three cases, as well as a study of the prevalence of T. beigelii colonization of normal-looking skin in patients receiving intensive chemotherapy for cancer.
Subject(s)
Dermatomycoses/pathology , Opportunistic Infections/pathology , Skin/pathology , Trichosporon , Adult , Biopsy , Humans , Immunocompromised Host , Male , Middle AgedABSTRACT
Fifty patients (42 men, 8 women) with seborrheic dermatitis were included in the trial. Ketoconazole 2% emulsion or the same emulsion without active drug was applied b.i.d. for 4 weeks. Two patients dropped out in the ketoconazole group and nine in the placebo group. Pityrosporum ovale was cultured from all patients at the start and from six out of 23 in the ketoconazole group versus nine out of 16 in the placebo group at week 4. The overall assessment showed a significantly better response to treatment for the ketoconazole emulsion (72%) than for the placebo (32%).
Subject(s)
Dermatitis, Seborrheic/drug therapy , Ketoconazole/therapeutic use , Dermatitis, Seborrheic/microbiology , Double-Blind Method , Emulsions , Female , Humans , Ketoconazole/administration & dosage , Malassezia/isolation & purification , MaleABSTRACT
In this study, the bioavailability of fluconazole and ketoconazole in the stratum corneum and oral mucosa after multiple oral dosages were compared. It is well recognized that such evaluation is heavily dependent on the test methods employed. A method of culture of fungi on human stratum corneum and superficial mucosal cells, a model close to the in-vivo situation, was used. A significant difference in the bioavailability of the two antifungals in stratum corneum and superficial cells of oral mucosa was found. The activity of ketoconazole proved to be more rapid against T. mentagrophytes while fluconazole appeared slightly more active against C. albicans.
Subject(s)
Fluconazole/pharmacokinetics , Ketoconazole/pharmacokinetics , Mouth Mucosa/metabolism , Skin/metabolism , Adult , Biological Availability , Candida albicans/drug effects , Humans , Trichophyton/drug effectsABSTRACT
Protothecosis is a rare infection by algae without chloroplasts. We report a case of an immunocompetent patient who had visited countries of south-east Asia. The lesion was present on the elbow, and consisted of a granuloma studded with cells and sporangiae of Prototheca. A computerized image analysis revealed large variations in the size and shape of the organisms. The lesion was successfully treated with itraconazole.
Subject(s)
Antifungal Agents/therapeutic use , Granuloma/etiology , Prototheca , Skin Diseases/etiology , Adult , Granuloma/drug therapy , Humans , Infections/etiology , Itraconazole , Ketoconazole/analogs & derivatives , Ketoconazole/therapeutic use , Male , Prototheca/ultrastructureABSTRACT
We have used a novel approach to in vitro culture of Candida albicans on cyanoacrylate skin surface strippings. It appears that with this model yeasts and hyphae grew on large surfaces of stratum corneum. The area of extension of the fungal growth was larger on stratum corneum taken from diabetic than from non-diabetic volunteers.
Subject(s)
Candida albicans/growth & development , Diabetes Mellitus , Skin/microbiology , Adult , Culture Techniques , HumansABSTRACT
We report a new case of mixed infection occurring at the same site of the skin in a human immune deficiency virus-positive patient. Hyperkeratotic and crusted erosions contained fusospirochetal organisms, Cryptococcus neoformans, and another unidentified fungus.
Subject(s)
Acquired Immunodeficiency Syndrome/complications , Skin Diseases, Infectious/etiology , Adult , Bacillus/isolation & purification , Biopsy , Cryptococcus neoformans/isolation & purification , Democratic Republic of the Congo , Humans , Leg Ulcer/etiology , Leg Ulcer/microbiology , Leg Ulcer/pathology , Male , Skin/pathology , Skin Diseases, Infectious/microbiology , Skin Diseases, Infectious/pathologySubject(s)
Dermatomycoses/etiology , Immunologic Deficiency Syndromes/complications , Opportunistic Infections/etiology , Trichosporon , Adult , Antifungal Agents/therapeutic use , Azoles/therapeutic use , Dermatomycoses/drug therapy , Humans , Ketoconazole/therapeutic use , Male , Middle Aged , Opportunistic Infections/drug therapyABSTRACT
Current in vitro testing methods for antifungal drugs are often inadequate and sometimes correlate poorly with in vivo efficacy. We present a method of culture of fungi on cyanoacrylate skin surface strippings that can be used in combination with computerized image analysis to evaluate the efficacy of commercially available topical and oral antifungal drugs. Since this method closely copies the in vivo situation, it has obvious advantages over other in vitro investigative procedures and should be of relevance clinically.
Subject(s)
Antifungal Agents/pharmacology , Biological Assay/methods , Fungi/drug effects , Culture Techniques/methods , Cyanoacrylates , Humans , Microbial Sensitivity Tests , Skin , Spores, Fungal/drug effectsABSTRACT
Lobomycosis is a rare disease occurring with predilection in the Amazonian region. We report the clinical and histological presentation of a patient living in Peru. The lesion resembled an hypertrophic scar located on the neck. It corresponded to a massive infiltration of the dermis by macrophages loaded with numerous Loboa loboi.
