Genome-Wide Associative Study of Phenotypic Parameters of the 3D Body Model of Aberdeen Angus Cattle with Multiple Depth Cameras.

In beef cattle breeding, genome-wide association studies (GWAS) using single nucleotide polymorphisms (SNPs) arrays can reveal many loci of various production traits, such as growth, productivity, and meat quality. With the development of genome sequencing technologies, new opportunities are opening up for more accurate identification of areas associated with these traits. This article aims to develop a novel approach to the lifetime evaluation of cattle by 3-D visualization of economic-biological and genetic features. The purpose of this study was to identify significant variants underlying differences in the qualitative characteristics of meat, using imputed data on the sequence of the entire genome. Samples of biomaterial of young Aberdeen-Angus breed cattle (n = 96) were the material for carrying out genome-wide SNP genotyping. Genotyping was performed using a high-density DNA chip Bovine GPU HD BeadChip (Illumina Inc., San Diego, CA, USA), containing ~150 thousand SNPs. The following indicators were selected as phenotypic features: chest width and chest girth retrieved by 3-D model and meat output on the bones. Correlation analysis showed a reliable positive relationship between chest width and meat output on the bones, which can potentially be used for lifetime evaluation of meat productivity of animals.

Coumarin's Anti-Quorum Sensing Activity Can Be Enhanced When Combined with Other Plant-Derived Small Molecules.

Coumarins are class of natural aromatic compounds based on benzopyrones (2H-1-benzopyran-2-ones). They are identified as secondary metabolites in about 150 different plant species. The ability of coumarins to inhibit cell-to-cell communication in bacterial communities (quorum sensing; QS) has been previously described. Coumarin and its derivatives in plant extracts are often found together with other small molecules that show anti-QS properties too. The aim of this study was to find the most effective combinations of coumarins and small plant-derived molecules identified in various plants extracts that inhibit QS in Chromobacterium violaceum ATCC 31532 violacein production bioassay. The coumarin and its derivatives: 7-hydroxycoumarin, 7.8-dihydroxy-4-methylcoumarin, were included in the study. Combinations of coumarins with gamma-octalactone, 4-hexyl-1.3-benzenediol, 3.4.5-trimethoxyphenol and vanillin, previously identified in oak bark (Quercus cortex), and eucalyptus leaves (Eucalyptus viminalis) extracts, were analyzed in a bioassay. When testing two-component compositions, it was shown that 7.8-dihydroxy-4-methylcoumarin, 4-hexyl-1.3-benzendiol, and gamma-octalactone showed a supra-additive anti-QS effect. Combinations of all three molecules resulted in a three- to five-fold reduction in the concentration of each compound needed to achieve EC50 (half maximal effective concentration) against QS in C. violaceum ATCC 31532.

Dataset on spatial variability of soil properties: Tokhmeyevo archaeological site of the Bronze Age, Chuvashia (southern fringe of the forest zone, the Russian Plain).

Geoarchaeological and palaeopedological studies focusing on the reconstruction of the Holocene paleoenvironments require a detailed knowledge of the spatial variability of soil properties both for the surface soils and paleosols buried under archaeological constructions. However, such studies are often carried out at unique sites where it is difficult to ensure the representativeness of the data obtained. In this paper, we report original data on 15 soil profiles which shows the range of spatial variability of soil properties (рН H2O, рН KCl, particle size distribution, depth of genetic horizons, colour codes) for both surface and buried soils at the Tokhmeyevo kurgan cemetery, located in the Middle Volga region, Chuvash Republic, Russia. The data supplement the original research [1] and also give additional detailed information on pollen and spore analysis by plant species for the humus horizons in four buried and one surface soils. All soils developed from the same lithology (mantle loam), at the same elevation, in a similar topographic position (levelled upland slope) and in proximity to each other. Both buried and surface soils, classified as Retisols [1], show slight variability in morphology and particle size distribution that varies in a similar range. However, the two soil groups (buried and surface) differ in two striking features: buried soils exhibit dark humus horizon and black humic cutans in the middle part of the soil profile; these features are absent in the surface soils. The values of рН in water and 1 M KCl suspension in the buried soils and soils of the kurgan mounds are lower than in the surface soils. The data on the spatial variation of the properties of the surface and buried soils increase the reliability of the results, making it possible to assess the extent to which the differences in soils are associated with the environmental evolution. The presented data can provide one the context for further work in paleoenvironmental studies and also be compared with other already published datasets increasing the reliability of conclusions about the trends of environmental evolution in the second half of the Holocene.

The effect of iron nanoparticles on performance of cognitive tasks in rats.

To assess the influence of 62.5 ± 0.6 nm iron nanoparticles on the status of central nervous system, a study was conducted on Wistar rats, which were subjected to abdominal injection of the studied nanoparticles at doses of 2 and 14 mg/kg. Based on the analysis of the structural and functional status of the cerebral cortex of rats, behavioral reactions of animals, and the elemental composition of the cerebral cortex, we investigated the nanoparticles' neurotoxic effect, whose degree and nature varied depending on the dosage and the time elapsed after the injection. We identified pathological changes in motor and somatosensory areas of the rats' cerebral cortex and established pronounced changes in the elemental homeostasis of the animals' cerebral cortex in experimental groups. Identified structural changes were accompanied by an increase in exploratory activity, locomotor activity, and emotional status of the animals. At that, these activities were more pronounced in rats, which were administered iron nanoparticles at a dose of 14 mg/kg. By the end of the experiment, the excitation processes prevailed over the inhibition processes that have led to the inhibition of central nervous system activity in experimental animals against the adaptation to stress in rats of the control group.