[Demonstration of the influenza virus A RNA by nucleic acid molecular hybridization using biotin-treated probes]. / Vyiavlenie RNK virusa grippa A metodom molekuliarnoi gibridizatsii nukleinovykh kislot s ispol'zovaniem biotinirovannykh zondov.

A probe containing full-size DNA copy of influenza A/USSR/90/70 virus protein gene M labeled with biotin on 32P was used for influenza A virus RNA detection by dot hybridization method. For labeling with biotin, a new method of its administration by chemical modification of nucleic acid was employed. In homologous DNA:DNA hybridization the sensitivity of determinations was less than 1 pg in the biotin-treatment of the probe and 1.25 pg in its radioactive labeling. Hybridization of DNA probe with cytoplasmic RNA isolated from influenza A virus-infected (strains A/USSR/90/77 and A/Texas/77) MDCK cells revealed RNA in the dot corresponding to 4.5-5.5 1g ID50 of virus present in 2 x 10(4) cells. The probe did not bind with negative controls in any dot in all the tests. The results of the study indicate that DNA probes labeled with biotin and 32P and used in dot hybridization for influenza A virus RNA detection in infected cells show the similar sensitivity and specificity.

[Use of probes containing cloned genes of the Karelian fever virus in detecting viral genetic material in infected cells by a molecular hybridization method]. / Ispol'zovanie zondov, soderzhashchikh klonirovannyye geny virusa karel'skov likhoradki, dlia vyiavleniia geneticheskogo materiala virusa v zarazhennykh kletkakh metodom molekuliarnoi gibridizatsii.

Two plasmid DNA-probes containing DNA-replicas of KFV genes (clone 1-protein E1 gene, clone 9--proteins E1 and P1 genes of KFV) were used for detection of the genetic material of Karelian fever virus (KFV) in the infected cells and study of the time course of accumulation of virus-specific RNAs in the process of infection. The detection was performed by the method of RNA:DNA dot-hybridization. Both probes were hybridized with KFV and Sindbis virus RNA in equal amounts--5 X 10(2) infected cells at the peak of virus infection (12 hours). None of the probes used could be bound with RNA of Venezuelan equine encephalomyelitis virus. The results obtained by the dot-hybridization method agree with previously published data on the antigenic relationship between Sindbis virus and KFV.

[Comparative analysis of the virus-specific proteins of cells infected with untypable herpes simplex virus strains]. / Sravnitel'nyi analiz virusspetsificheskikh belkov zarazhennykh netipiruiushchimisia shtammami virusa prostogo gerpesa kletok.

Herpes simplex viruses of the so-called intermediate types were typed by analysis of virus-specific proteins of the infected cells. The results obtained correlate well with the results of typing of these strains by DNA analysis using restriction endonucleases. Strain differences of virus-specific proteins of herpes simplex virus of both types were established.