ABSTRACT
A 13-year-old boy with tetralogy of Fallot and pulmonary atresia after a Waterston and Blalock-Taussig shunt underwent successful repair using a cryopreserved pulmonary homograft as a valved extracardiac conduit. Aortic transection gave a good operative field for pulmonary angioplasty and closure of the Waterston anastomosis. The cryopreserved pulmonary homograft provided excellent handling characteristics. The postoperative course was good, and postoperative echocardiography showed good function of the valved conduit.
Subject(s)
Pulmonary Artery/transplantation , Pulmonary Atresia/surgery , Tetralogy of Fallot/surgery , Adolescent , Anastomosis, Surgical , Cryopreservation , Hemodynamics , Humans , Male , Pulmonary Atresia/complications , Pulmonary Atresia/physiopathology , Tetralogy of Fallot/complications , Tetralogy of Fallot/physiopathology , Transplantation, Homologous , Vascular Surgical ProceduresABSTRACT
HLA-D/DR (Ia) glycoproteins were identified in human breast carcinoma and normal mammary gland cells by means of an anti-Ia monoclonal antibody. Two techniques were used: (1) immunoperoxidase staining performed on histological sections and (2) Ia glycoproteins were isolated as follows: firstly by radioactive labelling of isolated cells, then by filtration on Sephadex G25, followed by Lens culinaris chromatography, and immune complex formation and then elution on protein A-Sepharose. Lastly, the immune complex was studied by chromatofocusing. Both techniques revealed that Ia expression was found in carcinoma cells, but not in normal cells.
Subject(s)
Breast Neoplasms/immunology , Histocompatibility Antigens Class II/analysis , Adenofibroma/immunology , Antibodies, Monoclonal/immunology , Antigen-Antibody Complex/isolation & purification , Breast/immunology , Female , Glycoproteins/isolation & purification , HLA-DR Antigens , Histocompatibility Antigens Class II/isolation & purification , Humans , Immunoenzyme TechniquesABSTRACT
This study summarizes a one-year work experience in the area of human tumor stem cells cloning techniques. We found that it was more advisable to use an enzymatic method for dissociating solid tumors. The breast tumors possessing an unfavourable histoprognostic grade were found to grow up more easily in culture, which is a confirmation of known relationships between cell differentiation degree and cell proliferation. The high frequency of failures in the obtention of colonies did not allow to use this technique in daily clinical practice to study the in vitro sensitivity to drugs of a definite tumor. An improvement in the conditions of culture and the comparison with other in vitro methods for the prediction of response to oncostatics--particularly the incorporation of radioactive precursors (Livingston and coll., 1980)--will be attempted in future work.
