ABSTRACT
In the field of mine engineering, a cross-section topographic survey is usually carried out to perform volumetric calculations of earth movement in order to restore areas affected by extractive activities. Nowadays, Remote Sensing and Geographical Information System (GIS) technologies make it possible to perform the same work by using indirect methods such as images obtained by photogrammetric flights. In this context, Unmanned Aerial Systems (UAS) are considered a very convenient option to develop mapping projects in short periods of time and to provide quality geospatial information such as Digital Elevation Models (DEM) and orthophotos of centimetric spatial resolution. In the present study, this approach has been applied in a gravel extraction area to obtain data for estimating the filling volume of material required for the restoration of the relief (DEM(r)). The estimation of the DEM(r) is later used to calculate a difference of height values (DEM(r)-DEM) that will serve as a variable in the basic operation of volume calculation. The novelty of the presented method is the simulation of a relief adapted to the surrounding morphology, including the derived channel network and the visibility impact, improving what would be a simple clogging. Likewise, the generation of 3D models allows visualizing a new morphological structure of the relief. The proposed approach, based on GIS tools, allows analyzing water flow connectivity integration of the DEM(r) with the environment and estimating potential landscape impacts from the main focuses of a visual basin, both of which are key aspects of restoration modeling that are not always properly addressed.
ABSTRACT
We recently demonstrated that retinoic acid receptor-γ (RARγ) is overexpressed and acts as a tumor promoter in hepatocellular carcinoma (HCC). The oncogenic activity of RARγ is mainly attributed to its physiological interaction with p85α regulatory subunit of PI3K leading to constitutive activation of AKT. Here we report RARγ as a negative regulator of p53 signaling and thus extend the oncogenic potential of RARγ to a new role in controlling the balance between AKT and p53. A natural flavonoid acacetin is then identified to be capable of modulating RARγ-dependent AKT-p53 network. It specifically binds to RARγ and inhibits all-trans retinoic acid (atRA) stimulation of RARγ transactivation. However, the anticancer action of acacetin is independent on its modulation of RARγ-driven transcriptional activity. Acacetin induces cancer cell apoptosis through antagonizing the non-genomic effect of RARγ on AKT and p53. When bound to RARγ, acacetin prevents RARγ from its activation of AKT followed by recovery of the normal p53 signaling. Given the implication of AKT-p53 dysregulation in most HCC, targeting the non-genomic signaling of RARγ that switches AKT-p53 from a pro-survival to a pro-apoptotic program in cancer cells should be a promising strategy for developing novel anti-HCC drugs.
