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1.
J Agric Food Chem ; 51(10): 3073-8, 2003 May 07.
Article in English | MEDLINE | ID: mdl-12720394

ABSTRACT

The composition of the essential oil from ripe and unripe berries and leaves of Juniperus oxycedrus L. ssp. oxycedrus, Juniperus phoenicea ssp. turbinata and Juniperus communis ssp. communis was analyzed by GC-MS, and microbiological assays were carried out. Samples were collected in different localities (Sardinia, Italy) and hydro distilled. The yields ranged between 2.54% +/- 0.21 (v\w dried weight) and 0.04% +/- 0.00. A total of 36 components were identified. The major compounds in the essential oils were alpha-pinene, beta-pinene, delta-3-carene, sabinene, myrcene, beta-phellandrene, limonene, and D-germacrene. Both qualitative and quantitative differences between species and between different parts of the plant were observed. The essential oils and their major compounds were tested against Candida albicans, Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa, and the minimum inhibitory concentration and minimum bactericidal concentration were determined. The results obtained led to a nonsignificant inhibitory effect, although all the essential oils from Juniperus phoenicea ssp. turbinata and the essential oil from leaves of Juniperus oxycedrus ssp. oxycedrus exhibited rather good or weak activity against Candida albicans and Staphylococcus aureus.


Subject(s)
Anti-Infective Agents/pharmacology , Fruit/chemistry , Juniperus/chemistry , Oils, Volatile/chemistry , Oils, Volatile/pharmacology , Plant Leaves/chemistry , Anti-Bacterial Agents , Candida albicans/drug effects , Escherichia coli/drug effects , Gas Chromatography-Mass Spectrometry , Microbial Sensitivity Tests , Pseudomonas aeruginosa/drug effects , Staphylococcus aureus/drug effects
2.
J Agric Food Chem ; 51(4): 1030-4, 2003 Feb 12.
Article in English | MEDLINE | ID: mdl-12568568

ABSTRACT

The chemical composition of the essential oil of the Sardinian dwarf curry plant [Helichrysum italicum G. Don ssp. microphyllum (Willd) Nym] was studied. Genetic analysis suggested the presence of two chemotypes; morphological and chemical differences confirmed the presence of two chemotypes (A and B). The maximum yields were 0.18 and 0.04% (v/w) for flowering tops and stems, respectively. The concentrations of nerol and its esters (acetate and propionate), limonene, and linalool reach their highest values during the flowering stage both in flowers and in stems. Besides the essential oil, type B showed an interesting antifungal activity.


Subject(s)
Fungicides, Industrial/pharmacology , Helichrysum/chemistry , Helichrysum/genetics , Oils, Volatile/chemistry , Oils, Volatile/pharmacology , Acyclic Monoterpenes , Cyclohexenes , Flowers/chemistry , Helichrysum/growth & development , Limonene , Monoterpenes/analysis , Plant Oils/chemistry , Plant Oils/pharmacology , Plant Stems/chemistry , Terpenes/analysis
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