ABSTRACT
Patients with classical migraine investigated between attacks had significantly fewer platelet 3H-imipramine binding sites than control subjects and this finding was more marked in males than in females. There was no abnormality of binding characteristics of platelet 5-hydroxytryptamine receptors or of platelet alpha 2-adrenoceptors. Because the reduced 3H-imipramine binding capacity was found in classical migraineurs who were investigated between attacks, it may reflect a predisposition to migraine rather than being a consequence of attacks.
Subject(s)
Blood Platelets/metabolism , Carrier Proteins , Imipramine/blood , Migraine Disorders/blood , Receptors, Drug , Receptors, Neurotransmitter/metabolism , Receptors, Serotonin/metabolism , Female , Humans , Kinetics , Lysergic Acid Diethylamide/blood , Male , Receptors, Adrenergic/metabolism , Sex Factors , Yohimbine/bloodSubject(s)
Depressive Disorder/blood , Headache/blood , Serotonin/blood , Blood Platelets/metabolism , HumansABSTRACT
Four benzodiazepine radioimmunoassays are described, the most sensitive of which can detect sub-therapeutic levels of bromazepam, chlordiazepoxide, clobazam, demoxepam, desalkylflurazepam, desmethyldiazepam, diazepam, flunitrazepam, lorazepam, medazepam, nitrazepam, oxazepam, prazepam, temazepam and triazolam. The standard curve for diazepam has a concentration range of 0-2.5 ng ml-1. The assay is particularly applicable to blood samples of forensic interest that may be haemolysed or decomposing. A 75 microliter sample is required. The antiserum and [3H]flunitrazepam used are commercially available.
Subject(s)
Benzodiazepines/blood , Radioimmunoassay/methods , HumansABSTRACT
The radioimmunoassay of diazepam in biological fluids is described. The method is particularly applicable to samples encountered in forensic toxicology that may be haemolysed, decmposed and of small volume. The antiserum and tritiated diazepam that are used are commercially available. Polyethylene glycol (mol. wt 6000) is used to separate the bound and free fractons, and the free fracion is counted by extracting the tritiated diazepam into a toluene-based scintillant. Blood samples are extracted with ethyl acetate before analysis but urine samples may be assayed directly. The assay is convenient, quick and virtually specific for diazepam. The results correlate well with those obtained by gas chromatography.
