ABSTRACT
Stimulation of the nasal passages with ammonia vapors can initiate a nasopharyngeal response that resembles the diving response. This response consists of a sympathetically mediated increase in peripheral vascular resistance, parasympathetically mediated bradycardia and an apnea. The current study investigated the role of the anterior ethmoidal nerve (AEN) in the nasopharyngeal response in the rat, as it is thought that the AEN provides the main sensory innervation of the nasal passages. When both AENs were intact, nasal stimulation caused significant bradycardia, hypertension, and apnea and produced Fos label ventrally within the ipsilateral medullary dorsal horn (MDH) and paratrigeminal nucleus just caudal to the obex. This labeling presumably represents activation of second-order trigeminal neurons. When only one AEN was intact, the nasopharyngeal response was slightly attenuated, and a similar pattern of Fos labeling was only seen in the trigeminal nucleus ipsilateral to the intact AEN. The trigeminal labeling contralateral to the intact AEN was significantly reduced. When both AENs were cut, the nasopharyngeal response to nasal stimulation consisted of only a slight apnea and an increase in arterial pressure; the resultant Fos labeling within the trigeminal nucleus was significantly reduced. Cutting both AENs but not stimulating the nasal passages also produced some Fos labeling within the trigeminal nucleus. These findings suggest that a single AEN can provide sufficient afferent input to initiate the cardiorespiratory changes consistent with the nasopharyngeal response. We conclude that the AEN provides a unique afferent contribution that is capable of producing the diving response.
