ABSTRACT
Feline large granular lymphocyte lymphomas are rare but very aggressive tumors with a poor prognosis. In this study, a cell line from an abdominal effusion of a cat with large granular lymphoma was characterized. Immunophenotype staining was positive for CD3 and CD45R, and negative for CD4, CD8, CD56, CD79α, BLA.36 and NK1. A TCR γ gene rearrangement was detectable by PARR. Neither FeLV antigen nor exogenous FeLV provirus could be detected. A chromosomal instability associated with a centrosome hyperamplification could also be determined. The cell line is able to lyse target cells without antigen presentation or interaction with antigen presenting cells. Therefore, these cells were classified as genetically instable non-MHC-restricted cytotoxic T cells with large granular lymphocyte morphology.
Subject(s)
Cat Diseases/genetics , Cat Diseases/immunology , Cats/genetics , Cats/immunology , Lymphoma/veterinary , T-Lymphocytes, Cytotoxic/immunology , Amino Acid Sequence , Animals , Base Sequence , Cell Line, Tumor , Cytotoxicity, Immunologic , Female , Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor , Genes, p53 , Genomic Instability , Immunophenotyping , Leukemia Virus, Feline/isolation & purification , Lymphoma/genetics , Lymphoma/immunology , Microscopy, Electron, Transmission , RNA, Messenger/genetics , T-Lymphocytes, Cytotoxic/ultrastructureABSTRACT
Tryptic peptides of a feline large granular lymphoma cell line were analyzed by liquid chromatography electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS). Seventeen proteins of the T cell receptor signaling pathway could be identified by this approach. So far the existence of these proteins has only been postulated in the protein databases while experimental proof of their expression is predominantly pending. This article suggests where these proteins are located within the T cell receptor signaling pathway, thereby giving a short overview of the structure and function of this cascade.
