ABSTRACT
PURPOSE: Urocanic acid (UCA) is a major ultraviolet (UV)-absorbing endogenous chromophore in the epidermis and is also an efficacious immunosuppressant. The anti-inflammatory and cytoprotective effects of cis-UCA were studied in ocular surface cell cultures exposed to UV-B irradiation. METHODS: Human corneal epithelial cells (HCE-2) and human conjunctival epithelial cells (HCECs) were incubated with 10, 100, 1,000, and 5,000 microg/ml cis-UCA with and without a single UV-B irradiation dose. The concentrations of IL-1beta, IL-6, IL-8, and TNF-alpha in the culture medium and caspase-3 activity in the cell extract sampled were measured by enzyme-linked immunosorbent assay (ELISA). Cell viability was measured by the colorimetric MTT (3-(4,5-dimethyldiazol- 2-yl)-2,5-diphenyltetrazolium bromide) assay. RESULTS: UV-B irradiation multiplied interleukin IL-6 and IL-8 secretion levels in HCE-2 cells and HCECs as analyzed with ELISA. Cell viability as measured by the MTT assay declined by 30%-50% in HCE-2 cells and by 20%-40% in HCECs after UV-B irradiation. Moreover, UV-B increased caspase-3 activity in both cell types as analyzed with ELISA. Treatment with 100 microg/ml cis-UCA completely suppressed IL-6 and IL-8 secretion, decreased caspase-3 activity, and improved cell viability against UV-B irradiation. No significant effects on IL-6 or IL-8 secretion, caspase-3 activity, or viability of the non-irradiated cells were observed with 100 microg/ml cis-UCA in both cell types. The 5,000 microg/ml concentration was toxic. CONCLUSIONS: These findings indicate that cis-UCA may represent a promising anti-inflammatory and cytoprotective treatment option to suppress UV-B-induced inflammation and cellular damage in human corneal and conjunctival epithelial cells.
Subject(s)
Conjunctiva/cytology , Epithelial Cells/cytology , Epithelium, Corneal/cytology , Interleukin-6/metabolism , Interleukin-8/metabolism , Ultraviolet Rays , Urocanic Acid/pharmacology , Caspase 3/metabolism , Cell Death/drug effects , Cell Death/radiation effects , Cell Line , Cell Survival/drug effects , Cell Survival/radiation effects , Culture Media , Epithelial Cells/drug effects , Epithelial Cells/radiation effects , Humans , Isomerism , Urocanic Acid/chemistryABSTRACT
Magnetic signals produced by voluntary eye blinking were recorded. The maximal signals were found over the posterior parts of the orbits. The polarity of the signal reversed over each eye and in the frontal midline. The amplitude increases with light and amplitude decreases with darkness were similar in the electrical and magnetic blink and eye movement signals. Three different simulation models were used in the interpretation of the results. It is concluded that the primary current source of the blink signal is the transretinal current density. Lid movements change the geometry of the volume conductor resulting in changes in the measured electrical and magnetic field patterns.
