Characterization of sludge reduction and bacterial community dynamics in a pilot-scale multi-stage digester system with prolonged sludge retention time.

Sludge reduction performance and bacterial community dynamics in a pilot-scale multi-stage digester system with prolonged sludge retention time were characterized. Throughout the operation period of 281 days, the total loading sludge and the total digested sludge were 4700 and 3300 kg-MLSS. After 114 days of operation, the residual MLSS (RMLSS) in the reactors for sludge treatment was maintained at 18-25 kg-RMLSS m-3, and the sludge reduction efficiency achieved 95% under the F/M ratio (kg-loading MLSS kg-RMLSS-1) of less than 0.018. Also, among the sludge components, both fixed suspended solids and volatile suspended solids were reduced. Based on the sludge reduction performance and the RNA-based bacterial community characteristics, the combined action of the maintenance metabolism, lysis-cryptic growth, and particulate inorganic matter is proposed as the sludge reduction mechanism in the multi-stage sludge treatment process.

Simultaneous mitigation of methane and odors in a biowindow using a pipe network.

In this study, a biowindow with a piped gas collection network is proposed as an area-efficient landfill gas treatment system. A 9-m2 biowindow was constructed for treating landfill gas collected from an area of 450 m2 in a sanitary landfill, and its performance was evaluated for 224 days. The methane removal efficiency was 59-100% at 146.3-675.1 g-CH4 m-2 d-1. Odorous compounds were also removed by the biowindow, with a complex odor intensity removal rate of 93-100%. In particular, the removal efficiency for hydrogen sulfide and methanethiol, major contributors to the complex odor intensity, was 97% and 91%, respectively. Metagenomic analysis showed that the dominant bacterial genera shifted from Acinetobacter and Pseudomonas to Methylobacter and Methylocaldum due to the high concentration of methane. A high bacterial diversity was maintained, which may have contributed to the robust performance of the biowindow against environmental fluctuations. At 1/50th of the size of conventional biocovers, the proposed biowindow can greatly reduce the required installation area and represents a competitive method for the simultaneous treatment of methane and odor in landfills.

Odor mitigation and bacterial community dynamics in on-site biocovers at a sanitary landfill in South Korea.

Unpleasant odors emitted from landfills have been caused environmental and societal problems. For odor abatement, two pilot-scale biocovers were installed at a sanitary landfill site in South Korea. Biocovers PBC1 and PBC2 comprised a soil mixture with different ratios of earthworm casts as an inoculum source and were operated for 240 days. Their odor removal efficiencies were evaluated, and their bacterial community structures were characterized using pyrosequencing. In addition, the correlation between odor removability and bacterial community dynamics was assessed using network analysis. The removal efficiency of complex odor intensity in the two biocovers ranged from 81.1% to 97.8%. Removal efficiencies of sulfur-containing odors (hydrogen sulfide, methanethiol, dimethyl sulfide, and dimethyl disulfide), which contributed most to complex odor intensity, were greater than 91% in both biocovers. Despite the fluctuations in ambient temperature (-8.2 to 31.3 °C) and inlet complex odor intensity (10,000-42,748 of odor dilution ratio), biocovers PBC1 and PBC2 displayed stable deodorizing performance. A high ratio of earthworm casts as an inoculum source led to high odor removability during the first 25 days of operation, but different mixing ratios of earthworm casts did not significantly affect overall odor removability. A bacterial community analysis showed that Methylobacter, Arthrobacter, Acinetobacter, Rhodanobacter, and Pedobacter were the dominant genera in both biocovers. Network analysis results indicated that Steroidobacter, Cystobacter, Methylosarcina, Solirubrobacter, and Pseudoxanthomonas increased in relative abundance with time and were major contributors to odor removal, although these bacteria had a relatively low abundance compared to the overall bacterial community. These data contribute to a more comprehensive understanding of the relationship between bacterial community dynamics and deodorizing performance in biocovers.

Seasonal characteristics of odor and methane mitigation and the bacterial community dynamics in an on-site biocover at a sanitary landfill.

Landfills are key anthropogenic emission sources for odors and methane. For simultaneous mitigation of odors and methane emitted from landfills, a pilot-scale biocover (soil:perlite:earthworm cast:compost, 6:2:1:1, v/v) was constructed at a sanitary landfill in South Korea, and the biocover performance and its bacterial community dynamics were monitored for 240 days. The removal efficiencies of odor and methane were evaluated to compare the odor dilution ratios or methane concentrations at the biocover surface and landfill soil cover surface where the biocover was not installed. The odor removal efficiency was maintained above 85% in all seasons. The odor dilution ratios ranged from 300 to 3000 at the biocover surface, but they were 6694-20,801 at the landfill soil cover surface. Additionally, the methane removal efficiency was influenced by the ambient temperature; the methane removal efficiency in winter was 35-43%, while the methane removability was enhanced to 85%, 86%, and 96% in spring, early summer, and late summer, respectively. The ratio of methanotrophs to total bacterial community increased with increasing ambient temperature from 5.4% (in winter) to 12.8-14.8% (in summer). In winter, non-methanotrophs, such as Acinetobacter (8.8%), Rhodanobacter (7.5%), Pedobacter (7.5%), and Arthrobacter (5.7%), were abundant. However, in late summer, Methylobacter (8.8%), Methylocaldum (3.4%), Mycobacterium (1.1%), and Desulviicoccus (0.9%) were the dominant bacteria. Methylobacter was the dominant methanotroph in all seasons. These seasonal characteristics of the on-site biocover performance and its bacterial community are useful for designing a full-scale biocover for the simultaneous mitigation of odors and methane at landfills.

Rhizoremediation of diesel-contaminated soil using the plant growth-promoting rhizobacterium Gordonia sp. S2RP-17.

A plant growth-promoting rhizobacterium (PGPR) was isolated and identified as Gordonia sp. S2RP-17, which showed ACC deaminase and siderophore synthesizing activities. Its maximum specific growth rate was 0.54 ± 0.12 d(-1) at 5,000 mg L(-1) of total petroleum hydrocarbon (TPH), and its maximum diesel degradation rate was 2,434.0 ± 124.4 mg L(-1) d(-1) at 20,000 mg L(-1) of TPH. The growth of Zea mays was significantly promoted by the inoculation of Gordonia sp. S2RP-17 in the diesel-contaminated soil. Measured TPH removal efficiencies by various means were 13% by natural attenuation, 84.5% by planting Zea mays, and 95.8% by the combination of Zea mays and Gordonia sp. S2RP-17. The S2RP-17 cell counts were maintained at 1 × 10(6) CFU g-soil(-1) during the remediation period, although they slightly decreased from their initial numbers (2.94 × 10(7) CFU g-soil(-1)). These results indicate that rhizoremediation using both Zea mays and Gordonia sp. S2RP-17 is a promising strategy for enhancing remediation efficiency of diesel-contaminated soils.

Thermophilic biofiltration of H2S and isolation of a thermophilic and heterotrophic H2S-degrading bacterium, Bacillus sp. TSO3.

Thermophilic biofiltration of H(2)S-containing gas was studied at 60 degrees C using polyurethane (PU) cubes and as a packing material and compost as a source of thermophilic microorganisms. The performance of biofilter was enhanced by pH control and addition of yeast extract (YE). With YE supplement and pH control, H(2)S removal efficiency remained above 95% up to an inlet concentration of 950 ppmv at a space velocity (SV) of 50h(-1) (residence time=1.2 min). H(2)S removal efficiency strongly correlated with the inverse of H(2)S inlet concentrations and gas flow rates. Thermophilic, sulfur-oxidizing bacteria, TSO3, were isolated from the biofilter and identified as Bacillus sp., which had high similarity value (99%) with Bacillus thermoleovorans. The isolate TSO3 was able to degrade H(2)S without a lag period at 60 degrees C in liquid cultures as well as in the biofilter. High H(2)S removal efficiencies were sustained with a periodic addition of YE. This study demonstrated that an application of thermophilic microorganism for a treatment of hot gases may be an economically attractive option since expensive pre-cooling of gases to accommodate mesophilic processes is not required.