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1.
J Nurs Manag ; 26(8): 1024-1032, 2018 Nov.
Article in English | MEDLINE | ID: mdl-29923653

ABSTRACT

AIM: To investigate differences in work satisfaction and quality of nursing services between nurses from the nursing care integration service and general nursing units in Korea. BACKGROUND: The nursing care integration service was recently introduced in Korea to improve patient health outcomes through the provision of high quality nursing services and to relieve the caregiving burden of patients' families. METHODS: In this cross-sectional study, data were collected from a convenience sample of 116 and 156 nurses working in nursing care integration service and general units, respectively. The data were analysed using descriptive statistics, t tests and one-way analysis of variance. RESULTS: Regarding work satisfaction, nursing care integration service nurses scored higher than general unit nurses on professional status, autonomy and task requirements, but the overall scores showed no significant differences. Scores on overall quality of nursing services, responsiveness and assurance were higher for nursing care integration service nurses than for general unit nurses. CONCLUSIONS: Nursing care integration service nurses scored higher than general unit nurses on some aspects of work satisfaction and quality of nursing services. Further studies with larger sample sizes will contribute to improving the quality of nursing care integration service units. IMPLICATIONS FOR NURSING MANAGEMENT: These findings can help to establish strategies for the implementation and efficient operation of the nursing care integration service system, for the improvement of the quality of nursing services, and for successfully implementing and expanding nursing care integration service services in other countries.


Subject(s)
Job Satisfaction , Nursing Care/standards , Quality of Health Care/standards , Adult , Attitude of Health Personnel , Cross-Sectional Studies , Female , Humans , Male , Nursing Care/psychology , Republic of Korea , Surveys and Questionnaires , Workplace/psychology , Workplace/standards
2.
3.
Biomed Res Int ; 2016: 1473578, 2016.
Article in English | MEDLINE | ID: mdl-27563662

ABSTRACT

MicroRNA-122 (miRNA-122), also known as liver-specific miRNA, has recently been shown to be a potent biomarker in response to liver injury in mammals. The objective of this study was to examine its expression in response to toxicant treatment and acute liver damage, using the zebrafish system as an alternative model organism. For the hepatotoxicity assay, larval zebrafish were arrayed in 24-well plates. Adult zebrafish were also tested and arrayed in 200 mL cages. Animals were exposed to liver toxicants (tamoxifen or acetaminophen) at various doses, and miRNA-122 expression levels were analyzed using qRT-PCR in dissected liver, brain, heart, and intestine, separately. Our results showed no significant changes in miRNA-122 expression level in tamoxifen-treated larvae; however, miRNA-122 expression was highly induced in tamoxifen-treated adults in a tissue-specific manner. In addition, we observed a histological change in adult liver (0.5 µM) and cell death in larval liver (5 µM) at different doses of tamoxifen. These results indicated that miRNA-122 may be utilized as a liver-specific biomarker for acute liver toxicity in zebrafish.


Subject(s)
Biological Assay/methods , Chemical and Drug Induced Liver Injury/genetics , Drug Evaluation, Preclinical/methods , MicroRNAs/genetics , Toxicity Tests/methods , Zebrafish/genetics , Acetaminophen/toxicity , Animals , Biomarkers/analysis , Tamoxifen
4.
Eur J Med Chem ; 101: 716-35, 2015 Aug 28.
Article in English | MEDLINE | ID: mdl-26218650

ABSTRACT

We have developed a series of adamantane carboxylic acid derivatives exhibiting potent diacylglycerol acyltransferase 1 (DGAT1) inhibitory activities. Optimization of the series led to the discovery of E-adamantane carboxylic acid compound 43c, which showed excellent in vitro activity with an IC50 value of 5 nM against human and mouse DGAT1, also good druggability as well as microsomal stability and safety profiles such as hERG, CYP and cytotoxicity. Compound 43c significantly reduced plasma triglyceride levels in vivo (in rodents and zebrafish) and also showed bodyweight gain reduction and glucose area under curve (AUC) lowering efficacy in diet-induced obesity (DIO) mice.


Subject(s)
Adamantane/analogs & derivatives , Diabetes Mellitus, Experimental/drug therapy , Diacylglycerol O-Acyltransferase/antagonists & inhibitors , Drug Discovery , Enzyme Inhibitors/chemical synthesis , Enzyme Inhibitors/pharmacology , Obesity/drug therapy , Adamantane/chemistry , Adamantane/pharmacology , Animals , Diabetes Mellitus, Experimental/enzymology , Diacylglycerol O-Acyltransferase/metabolism , Diet, High-Fat/adverse effects , Dose-Response Relationship, Drug , Enzyme Inhibitors/chemistry , Humans , Male , Mice , Mice, Inbred C57BL , Molecular Structure , Obesity/enzymology , Structure-Activity Relationship , Zebrafish
5.
Hum Mol Genet ; 24(17): 4848-61, 2015 Sep 01.
Article in English | MEDLINE | ID: mdl-26056227

ABSTRACT

Miles-Carpenter syndrome (MCS) was described in 1991 as an XLID syndrome with fingertip arches and contractures and mapped to proximal Xq. Patients had microcephaly, short stature, mild spasticity, thoracic scoliosis, hyperextendable MCP joints, rocker-bottom feet, hyperextended elbows and knees. A mutation, p.L66H, in ZC4H2, was identified in a XLID re-sequencing project. Additional screening of linked families and next generation sequencing of XLID families identified three ZC4H2 mutations: p.R18K, p.R213W and p.V75in15aa. The families shared some relevant clinical features. In silico modeling of the mutant proteins indicated all alterations would destabilize the protein. Knockout mutations in zc4h2 were created in zebrafish and homozygous mutant larvae exhibited abnormal swimming, increased twitching, defective eye movement and pectoral fin contractures. Because several of the behavioral defects were consistent with hyperactivity, we examined the underlying neuronal defects and found that sensory neurons and motoneurons appeared normal. However, we observed a striking reduction in GABAergic interneurons. Analysis of cell-type-specific markers showed a specific loss of V2 interneurons in the brain and spinal cord, likely arising from mis-specification of neural progenitors. Injected human wt ZC4H2 rescued the mutant phenotype. Mutant zebrafish injected with human p.L66H or p.R213W mRNA failed to be rescued, while the p.R18K mRNA was able to rescue the interneuron defect. Our findings clearly support ZC4H2 as a novel XLID gene with a required function in interneuron development. Loss of function of ZC4H2 thus likely results in altered connectivity of many brain and spinal circuits.


Subject(s)
Carrier Proteins/genetics , Central Nervous System/cytology , Central Nervous System/metabolism , Interneurons/metabolism , Animals , COS Cells , Cell Line , Chlorocebus aethiops , Computational Biology , Female , Gene Expression , Genes, X-Linked , Humans , Intracellular Signaling Peptides and Proteins , Male , Mutation , Nuclear Proteins , Organ Specificity/genetics , Pedigree , Zebrafish
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