Dispersive biofilm from membrane bioreactor strains: effects of diffusible signal factor addition and characterization by dispersion index.

Introduction: Biofilm occurs ubiquitously in water system. Excessive biofilm formation deteriorates severely system performance in several water and wastewater treatment processes. Quorum sensing systems were controlled in this study with a signal compound cis-2-Decenoic acid (CDA) to regulate various functions of microbial communities, including motility, enzyme production, and extracellular polymeric substance (EPS) production in biofilm. Methods: The addition of CDA to six strains extracted from membrane bioreactor sludge and the Pseudomonas aeruginosa PAO1 strain was examined for modulating biofilm development by regulating DSF expression. Results and discussion: As the CDA doses increased, optical density of the biofilm dispersion assay increased, and the decrease in EPS of the biofilm was obvious on membrane surfaces. The three-dimensional visual images and quantitative analyses of biofilm formation with CDA proved thinner, less massive, and more dispersive than those without; to evaluate its dispersive intensity, a dispersion index was proposed. This could compare the dispersive effects of CDA dosing to other biofilms or efficiencies of biofouling control practices such as backwashing or new cleaning methods.

Humic acid removal and microbial community function in membrane bioreactor.

A membrane bioreactor with humic acid substrate (MBR-H) was operated to investigate organic removal and membrane performance. Approximately, 60% of chemical oxygen demand removal was observed in MBR-H. The biosorption capacity reached to the maximum value of 29.2 mg g-1 in the experiments with various activated sludge concentrations and the amount adsorbed on the newly produced microbes was limited. To understand key functions of microorganisms in the biodegradation of humic acid, the microbial community was examined. The dominant phylum was changed from Actinobacteria at the raw sludge to Proteobacteria at the MBR-H. Especially, great increases of ß-, γ-, and δ-Proteobacteria in the MBR-H indicated that those class of Proteobacteria played a vital role in humic acid removal. Investigation at the genus level showed enrichment of Stenotrophobacter in the MBR-H, which indicated the presence of metabolites in the proposed humic substance degradation pathway. In addition, the bacteria producing extracellular polymeric substances were increased in the MBR-H. Substantial variation of microbial community function was occurred in the MBR to degrade humic acid. Operational parameters in MBRs might be sought to maintain water permeability and to obtain preferable condition to evolution of microbial consortia for degradation of the refractory organic matter.

Characteristics of organic fouling, reversibility by physical cleaning and concentrates in forward osmosis membrane processes for wastewater reclamation.

Numerous advantages of forward osmosis (FO) include operation at low or no hydraulic pressure, high rejection of a wide range of contaminants, and low irreversible fouling. The FO has been investigated to reduce effluent discharge in wastewater reclamation. The application of wastewater effluent as a feed stream to FO yields fouling on the active layer of the FO membrane. Fouling was examined using two compounds (i.e., alginate and humic acid) with distinguished hydrophobic properties. The repeated filtration and surface wash were applied and flux decline and reversibility of physical cleaning were evaluated. In addition, the characteristics of fouling cakes and concentrates were also analyzed. The foulants showed different behaviors in flux decline. The thick cake layer of alginate was obvious and the cake enhanced concentration polarization was also observed. The recovery results along with the FTIR spectra and FE-SEM images proved that the surface cleaning was not effective to detach foulants, especially for alginate fouling. The osmotic backwash showed greater flux recovery for alginate fouling than humic acid fouling, which indicated that restoring membrane pores or disturbing cake layers by osmotic backwash might be successful for the foulants for strong interactions between foulants and foulants. The concentrates were mostly composed of humic substances and low-molecular weight neutrals. The differences in the relative portions of the major components were occurred in the concentrates implying that the organic properties of the feed water and also interactions of foulants and membranes should be evaluated prior to determination of disposal options for concentrates.

Monitoring biofouling based on aerobic respiration in reverse osmosis system.

A monitoring method of biofouling in reverse osmosis (RO) system was proposed based on the fluorescent signal of resorufin, which is reduced by nicotinamide adenine dinucleotide released from viable cells during aerobic respiration. The fluorescent signal of resorufin reduced by planktonic cells and microorganisms of biofilm showed linearity, indicating its feasibility to monitor biofouling in a RO system. For the application of the method to the lab-scale RO system, the injection concentration of resazurin and the injection flow rate were optimized. Biofilm on RO membranes continuously operated in a lab-scale RO system was estimated by resorufin fluorescence under optimized detection condition. As a result, resorufin fluorescence on RO membrane showed a significant increase in which the permeability of RO system decreased by 30.48%. Moreover, it represented the development of biofilm as much as conventional biofilm parameters such as adenosine triphosphate, extracellular polymeric substances, and biofilm thickness. The proposed method could be used as a sensitive and low-cost technology to monitor biofouling without autopsy of membranes.

Design and QoS of a wireless system for real-time remote electrocardiography.

Quality of service (QoS) and, in particular, reliability and a bounded low latency are essential attributes of safety-critical wireless systems for medical applications. However, wireless links are typically prone to bursts of errors, with characteristics which vary over time.We propose a wireless system suitable for real-time remote patient monitoring in which the necessary reliability and guaranteed latency are both achieved by an efficient error control scheme. We have paired an example remote electrocardiography application to this wireless system. We also developed a tool chain that uses a formal description of the proposed wireless medical system architecture in the architecture analysis and design language to assess various combinations of system parameters: we can determine the QoS in terms of packet-delivery ratio and the service latency, and also the size of jitter buffer required for seamless ECG monitoring. A realistic assessment, based on data from the MIT-BIT arrhythmia database, shows that the proposed wireless system can achieve an appropriate level of QoS for real-time ECG monitoring if link-level error control is correctly implemented. Additionally, we present guidelines for the design of energy-efficient link-level error control, derived from energy data, obtained from simulations.