Redox-enabled electronic interrogation and feedback control of hierarchical and networked biological systems.

Microelectronic devices can directly communicate with biology, as electronic information can be transmitted via redox reactions within biological systems. By engineering biology's native redox networks, we enable electronic interrogation and control of biological systems at several hierarchical levels: proteins, cells, and cell consortia. First, electro-biofabrication facilitates on-device biological component assembly. Then, electrode-actuated redox data transmission and redox-linked synthetic biology allows programming of enzyme activity and closed-loop electrogenetic control of cellular function. Specifically, horseradish peroxidase is assembled onto interdigitated electrodes where electrode-generated hydrogen peroxide controls its activity. E. coli's stress response regulon, oxyRS, is rewired to enable algorithm-based feedback control of gene expression, including an eCRISPR module that switches cell-cell quorum sensing communication from one autoinducer to another-creating an electronically controlled 'bilingual' cell. Then, these disparate redox-guided devices are wirelessly connected, enabling real-time communication and user-based control. We suggest these methodologies will help us to better understand and develop sophisticated control for biology.

Network-based redox communication between abiotic interactive materials.

Recent observations that abiotic materials can engage in redox-based interactive communication motivates the search for new redox-active materials. Here we fabricated a hydrogel from a four-armed thiolated polyethylene glycol (PEG-SH) and the bacterial metabolite, pyocyanin (PYO). We show that: (i) the PYO-PEG hydrogel is reversibly redox-active; (ii) the molecular-switching and directed electron flow within this PYO-PEG hydrogel requires both a thermodynamic driving force (i.e., potential difference) and diffusible electron carriers that serve as nodes in a redox network; (iii) this redox-switching and electron flow is controlled by the redox network's topology; and (iv) the ability of the PYO-PEG hydrogel to "transmit" electrons to a second insoluble redox-active material (i.e., a catechol-PEG hydrogel) is context-dependent (i.e., dependent on thermodynamic driving forces and appropriate redox shuttles). These studies provide an experimental demonstration of important features of redox-communication and also suggest technological opportunities for the fabrication of interactive materials.

Pediatric Otoscopy Video Screening With Shift Contrastive Anomaly Detection.

Ear related concerns and symptoms represent the leading indication for seeking pediatric healthcare attention. Despite the high incidence of such encounters, the diagnostic process of commonly encountered diseases of the middle and external presents a significant challenge. Much of this challenge stems from the lack of cost effective diagnostic testing, which necessitates the presence or absence of ear pathology to be determined clinically. Research has, however, demonstrated considerable variation among clinicians in their ability to accurately diagnose and consequently manage ear pathology. With recent advances in computer vision and machine learning, there is an increasing interest in helping clinicians to accurately diagnose middle and external ear pathology with computer-aided systems. It has been shown that AI has the capacity to analyze a single clinical image captured during the examination of the ear canal and eardrum from which it can determine the likelihood of a pathognomonic pattern for a specific diagnosis being present. The capture of such an image can, however, be challenging especially to inexperienced clinicians. To help mitigate this technical challenge, we have developed and tested a method using video sequences. The videos were collected using a commercially available otoscope smartphone attachment in an urban, tertiary-care pediatric emergency department. We present a two stage method that first, identifies valid frames by detecting and extracting ear drum patches from the video sequence, and second, performs the proposed shift contrastive anomaly detection (SCAD) to flag the otoscopy video sequences as normal or abnormal. Our method achieves an AUROC of 88.0% on the patient level and also outperforms the average of a group of 25 clinicians in a comparative study, which is the largest of such published to date. We conclude that the presented method achieves a promising first step toward the automated analysis of otoscopy video.

Light field camera study of near-ground turbulence anisotropy and observation of small outer-scales.

Understanding turbulence effects on laser beam propagation is critical to the emerging design, study, and test of many long-range free space optical (FSO) communication and directed energy systems. Conventional studies make the prevalent assumption of isotropic turbulence, while more recent results suggest anisotropic turbulence for atmospheric channels within a few meters elevation above the ground. As countless FSO systems have been and continue to be deployed in such channels, analysis of anisotropic modelings has become one of the fastest growing areas in FSO research. This in turn motivates new tools that can distinguish anisotropic characteristics to improve both modeling accuracy and physical interpretations. Wavefront sensors such as Shack-Hartmann sensors, interferometers, and plenoptic sensors have been devised and used in experiments; however, they all require rigid alignments that lack resilience against temperature gradient buildup and beam wander. We find that by using a light field camera (LFC) that extracts perturbation of individual light rays, the wave structure function of turbulence can be retrieved with high reliability. Furthermore, we find through experiments that the outer scales of near-ground turbulence tend to be a magnitude smaller than conventional theoretical assumptions, agreeing with new findings by others but being absent in current theoretical modelings. As a result, we believe that the LFC is an ideal candidate in the frontier of turbulence research; it is both commercially available and easy to adapt to turbulence experiments.

Measuring the turbulence profile in the lower atmospheric boundary layer.

Optical turbulence can have a severe effect on the propagation of laser beams through the atmosphere. In free space optics and directed energy applications, these laser beams quite often propagate along a slant or vertical path. In these cases, the refractive index structure function parameter cannot be assumed constant, since it varies with height. How it varies with height, especially in the first few meters above the ground, is not well behaved. Turbulence height profiles have been measured since the 1970s, mainly for astronomical observations. These profiles are usually measured for the atmospheric boundary layer (the layer of air from the ground up to approx. 1 km during day and 100 m during night) and some kilometers above it. We have measured the temperature fluctuations in the first few meters above ground level using a system containing eight resistance thermometer devices, mounted in a row at different spacings. Measurements were made flying this system under a tethered balloon or mounted on a telescoping mast. The temperature structure function parameter, CT2, can be estimated from the temperature fluctuations measured by the 28 different probe pairs and the unique distances between the two probes. Finally, Cn2 is estimated from this temperature structure function parameter and compared to values predicted by a turbulence profile model.

Using turbulence scintillation to assist object ranging from a single camera viewpoint.

Image distortions caused by atmospheric turbulence are often treated as unwanted noise or errors in many image processing studies. Our study, however, shows that in certain scenarios the turbulence distortion can be very helpful in enhancing image processing results. This paper describes a novel approach that uses the scintillation traits recorded on a video clip to perform object ranging with reasonable accuracy from a single camera viewpoint. Conventionally, a single camera would be confused by the perspective viewing problem, where a large object far away looks the same as a small object close by. When the atmospheric turbulence phenomenon is considered, the edge or texture pixels of an object tend to scintillate and vary more with increased distance. This turbulence induced signature can be quantitatively analyzed to achieve object ranging with reasonable accuracy. Despite the inevitable fact that turbulence will cause random blurring and deformation of imaging results, it also offers convenient solutions to some remote sensing and machine vision problems, which would otherwise be difficult.

Phase and amplitude beam shaping with two deformable mirrors implementing input plane and Fourier plane phase modifications.

We find that ideas in optical image encryption can be very useful for adaptive optics in achieving simultaneous phase and amplitude shaping of a laser beam. An adaptive optics system with simultaneous phase and amplitude shaping ability is very desirable for atmospheric turbulence compensation. Atmospheric turbulence-induced beam distortions can jeopardize the effectiveness of optical power delivery for directed-energy systems and optical information delivery for free-space optical communication systems. In this paper, a prototype adaptive optics system is proposed based on a famous image encryption structure. The major change is to replace the two random phase plates at the input plane and Fourier plane of the encryption system, respectively, with two deformable mirrors that perform on-demand phase modulations. A Gaussian beam is used as an input to replace the conventional image input. We show through theory, simulation, and experiments that the slightly modified image encryption system can be used to achieve arbitrary phase and amplitude beam shaping within the limits of stroke range and influence function of the deformable mirrors. In application, the proposed technique can be used to perform mode conversion between optical beams, generate structured light signals for imaging and scanning, and compensate atmospheric turbulence-induced phase and amplitude beam distortions.

Multi-aperture laser transmissometer system for long-path aerosol extinction rate measurement.

We present the theory, design, simulation, and experimental evaluations of a new laser transmissometer system for aerosol extinction rate measurement over long paths. The transmitter emits an ON/OFF modulated Gaussian beam that does not require strict collimation. The receiver uses multiple point detectors to sample the sub-aperture irradiance of the arriving beam. The sparse detector arrangement makes our transmissometer system immune to turbulence-induced beam distortion and beam wander caused by the atmospheric channel. Turbulence effects often cause spatial discrepancies in beam propagation and lead to miscalculation of true power loss when using the conventional approach of measuring the total beam power directly with a large-aperture optical concentrator. Our transmissometer system, on the other hand, combines the readouts from distributed detectors to rule out turbulence-induced temporal power fluctuations. As a result, we show through both simulation and field experiments that our transmissometer system works accurately with turbulence strength Cn2 up to 10-12 m-2/3 over a typical 1-km atmospheric channel. In application, our turbulence- and weather-resistant laser transmissometer system has significant advantages for the measurement and study of aerosol concentration, absorption, and scattering properties, which are crucial for directed energy systems, ground-level free-space optical communication systems, environmental monitoring, and weather forecasting.

Omnidirectional beacon-localization using a catadioptric system.

We present a catadioptric beacon localization system that can provide mobile network nodes with omnidirectional situational awareness of neighboring nodes. In this system, a receiver composed of a hyperboloidal mirror and camera is used to estimate the azimuth, elevation, and range of an LED beacon. We provide a general framework for understanding the propagation of error in the angle-of-arrival estimation and then present an experimental realization of such a system. The situational awareness provided by the proposed system can enable the alignment of communication nodes in an optical wireless network, which may be particularly useful in addressing RF-denied environments.

Long-range vibration detection system using heterodyne interferometry.

We present the design and performance of an extremely sensitive coherent remote vibration detection system using optical heterodyne vibration of phase shifts produced by laser light scattered off a remote target. The resulting phase-modulated intermediate RF of 200 MHz, which carries the vibrational motion of the target, is demodulated down to baseband using an RF in-phase and quadrature demodulator. Acquisition and calculation of the target phase angle is carried out on a custom-made control board which utilizes high-resolution A/D converters, variable gain amplifiers, and a Spartan-6 field programmable gate array.