Evaluation of the Anti-microbial Efficacy of a Novel Endodontic Irrigant Against Enterococcus faecalis: An In Vitro Study.

INTRODUCTION: Enterococcus faecalis is a constant microbiome that plays an inevitable role in the etiology of peri radicular lesions after endodontic treatment, chronic and, apical periodontitis and, recently, in periimplantitis. The effective biomechanical preparation and the use of potent irrigating solutions will permit bacterial neutralization and toxin inactivation, leading to the success of endodontic treatment. This study aimed to evaluate the "anti-microbial" efficacy of simvastatin (SMV) against E. faecalis as an endodontic irrigant. MATERIALS AND METHODS: In this invitro experimental study, the antimicrobial efficacy of SMV was evaluated against E. faecalis using the agar diffusion method. The samples were divided randomly into the following groups. GROUP 1: SMV solution 1 µM/L concentration, GROUP 2: SMV solution 5 µM/L concentration, GROUP 3: SMV solution 10 µM/L concentration, GROUP 4: 2% chlorhexidine gluconate (CHX) solution (positive control), and GROUP 5: normal saline (negative control). Linear measurement was done by measuring the zones of inhibition around the medicaments in the cavities in millimeters. Results were tabulated. RESULTS: The results of the study have shown the zone of inhibition of Group 4 (2% CHX solution) is 19 mm, which demonstrated the best outcome. When comparing the test samples, Group 3 (SMV solution 10 M/L concentration) has the best zone of inhibition, measuring 14 mm, followed by Group 2 (SMV solution 5 M/L concentration), which is 9 mm. CONCLUSION: The results of this in vitro study have proven that SMV's anti-microbial activity, albeit less potent than CHX in this in vitro investigation, has demonstrated that it can be utilized as an efficient endodontic irrigant.

Pulp Stones: Diagnostic Significance in Early Diagnosis and Radiographic Correlation with Ischemic Heart Diseases.

Context and Aim Sufficient evidence exists in the literature which indicates that patients with ischemic heart diseases (IHDs) show higher degree of pulp calcifications. The present study was, therefore, planned to estimate the prevalence of pulp stones in patients diagnosed with/or undergoing treatment for IHDs. Materials and Methods The present study, which consisted of 300 subjects within the age range of 25 to 65 years, was divided into two groups: study group comprising 150 patients (113 males and 37 females) and 150 age- and sex-matched healthy controls. Pulp stones were imaged using bitewing radiographs and paralleling technique under standard conditions. Statistical Analysis Used Statistical analysis was done using IBM SPSS Statistics for Windows version 21 (IBM Corp, Armonk, USA), while independent t -test and Chi-square test were done to check the prevalence of pulp stones in the study and control groups, based on gender-, arch-, region- and side-wise distribution. p < 0.05 was considered statistically significant. Results The patients with IHDs exhibited 100% prevalence of pulp stones and the difference was found to be statistically significant, although there was a significant difference in the mean number of pulp stones observed in the study and control groups, with the study group revealing 2217 pulp stones as against 639 pulp stones observed in the control group ( p < 0.001). Furthermore, maxillary arch and posterior teeth were predominantly affected in both the groups, although the difference was not found to be statistically significant. Conclusions The findings of the present study suggested a positive correlation between pulp stones and IHDs, highlighting the significance of dental radiographic examination, which may have a possibility for an early detection of IHDs.

Perioperative versus postoperative chemotherapy for gastric cancer: A propensity score matched analysis.

AIM: In advanced gastric cancer, chemotherapy, given either perioperatively or as an adjuvant treatment, has been shown to improve survival when compared to surgery alone. However, no trial has compared these two approaches head-to-head. Hence, we aimed to compare the short- and long-term outcomes of patients with gastric cancer who received either perioperative chemotherapy or adjuvant chemotherapy. METHODS: Retrospective analysis of patients with gastric cancers treated from 2010 to 2016. Using propensity score matching, resected patients who received perioperative chemotherapy were matched for histology, nodal dissection, and extent of surgery with another cohort of patients who received only adjuvant chemotherapy to create two matched groups of 101 patients each-group A (perioperative) and group B (adjuvant)-and the outcomes were compared between them. RESULTS: The patient demographics were evenly distributed in the two groups. There was no difference in the median number of chemotherapy cycles delivered (6 vs 6, P = .8) or the grade 3-4 toxicity (17.2% vs 12.1%, P = .26) in group A and group B, respectively. We could not demonstrate a significant difference in the postoperative mortality (2.6% vs 0%) or overall postoperative complications (23% vs 19%) between groups A and B. The overall recurrence rate (37% vs 42%), 3-year disease-free survival rate (51% vs 48%), and 3-year overall survival rate (53% vs 55%) were not significantly different in group A and group B, respectively. CONCLUSIONS: We were unable to detect a significant difference in the short-term or long-term outcomes of patients with gastric cancer undergoing either perioperative or adjuvant chemotherapy.

The tumor suppressor RASSF1A is a novel effector of small G protein Rap1A

Rap1A is a small G protein implicated in a spectrum of biological processes such as cell proliferation, adhesion, differentiation, and embryogenesis. The downstream effectors through which Rap1A mediates its diverse effects are largely unknown. Here we show that Rap1A, but not the related small G proteins Rap2 or Ras, binds the tumor suppressor Ras association domain family 1A (RASSF1A) in a manner that is regulated by phosphorylation of RASSF1A. Interaction with Rap1A is shown to influence the effect of RASSF1A on microtubule behavior.