KCNH1 potassium channels are expressed in cervical cytologies from pregnant patients and are regulated by progesterone.

Potassium voltage-gated channel, subfamily H (eag-related), member 1 (KCNH1) potassium channels are potential tumour markers and cancer therapeutic targets and are up-regulated by oestrogens and human papilloma virus (HPV) oncogenes. However, the role of KCNH1 in normal tissues is poorly understood, and its expression in pregnancy is unknown. We wondered whether KCNH1 channels are expressed in cervical cells from pregnant patients and whether progesterone (P4) regulates KCNH1. The association with HPV was also investigated. KCNH1 protein expression was studied by immunocytochemistry in liquid-based cervical cytologies; 93 samples were obtained from pregnant patients at different trimesters, and 15 samples were obtained from non-pregnant women (controls). The presence of HPV was studied by PCR with direct sequencing and nested multiplex PCR. HeLa cervical cancer cells were transfected with human progesterone receptor-B (PR-B) and treated with P4. KCNH1 mRNA expression in these cultures was studied by real-time PCR. KCNH1 protein was detected in 100% of the pregnancy samples and in 26% of the controls. We found 18 pregnant patients infected with HPV and detected 14 types of HPV. There was no association between the percentage of cells expressing KCNH1 and either the presence or type of HPV. P4 induced KCNH1 mRNA and protein expression in cells transfected with human PR-B. No regulation of KCNH1 by P4 was observed in non-transfected cells. We show for the first time the expression of an ion channel during human pregnancy at different trimesters and KCNH1 regulation by P4 in human cells. These data raise a new research field for KCNH1 channels in human tissues.

Medida del grosor foveolar mediante tomografía de coherencia óptica en pacientes adultos con nefropatía diabética en hemodiálisis / Measurement of foveal thickness by optical coherence tomography in adult haemodialysis patients with diabetic nephropathy

Antecedentes: Diversos estudios han demostrado la eficacia de la hemodiálisis (HD) sobre el edema macular de los pacientes diabéticos. Objetivo: Estudiar los efectos de una sesión de HD sobre el grosor foveolar, mediante tomografía de coherencia óptica (OCT), en pacientes adultos con diabetes mellitus tipo 2 con insuficiencia renal crónica (IRC)estadio 5 secundaria a nefropatía diabética en HD. Pacientes y métodos: Se estudiaron 25 ojos de 14 pacientes a los cuales se les realizó analítica y OCT pre-HD y post-HD. Resultados: Como grupo, el grosor foveolar no se modificaba tras una sesión de HD en los 25 ojos estudiados (245,28± 52,21 µ frente a 240,40 ± 40,25 µ) (p = 0,428) (2% de reducción) ni se correlacionaba con ninguno de los parámetros clínicos o analíticos analizados. Al comparar el subgrupo de 13 ojos en los que el grosor foveolar no se modificaba o disminuía respecto al subgrupo de 12 ojos en los que el grosor foveolar aumentaba se encontró que en el primer subgrupo la temperatura del baño era significativamente mayor (37,00 ± 0,00 frente a 36,29 ºC, p = 0,008) y la conductividad significativamente menor (14,00 ± 0,00 frente a 14,29 ± 0,10 mS/cm, p = 0,030). Conclusión: La HD podría modificar el grosor foveolar retiniano en función de la modificación de parámetros como la temperatura del baño yl a conductividad (AU)

Background: Several studies have demonstrated the efficacy of hemodialysis (HD) on macular edema in diabetic patients. Objective: To study the effects of a HD session on foveal thickness by optical coherence tomography (OCT) in adult patients with type 2 diabetes mellitus with chronic renal failure (CRF)secondary to stage 5 diabetic nephropathy in HD. Patients and methods: We studied 25 eyes of 14 patients who underwent analytical studies and pre-HD and post-HD OCT. Results: As a group, the foveal thickness did not change afterone session of HD in the 25 eyes studied (245.28 ± 52.21 Ì versus 240.40 ± 40.25 µ) (p = 0.428) (2% reduction) or correlated with any clinical or laboratory parameters analyzed. When comparing the subgroup of 13 eyes in which the foveal thickness did not change or decreased compared to the subgroup of 12 eyes in which the foveal thickness increased we found that in the first subgroup the bath temperature was significantly higher (37.00 ± 0.00 versus 36.29 °C, p = 0.008) and the conductivity was significantly lower (14.00 ± 0.00 versus 14.29± 0.10 mS/cm, p = 0.030). Conclusion: HD may modify the foveal retinal thickness as a function of changing parameters such as bath temperature and conductivity. Conclusion: HD may modify the foveal retinal thickness as a function of changing parameters such as bath temperature and conductivity (AU)

Measurement of foveal thickness by optical coherence tomography in adult haemodialysis patients with diabetic nephropathy.

BACKGROUND: Several studies have demonstrated the efficacy of hemodialysis (HD) on macular edema in diabetic patients. OBJECTIVE: To study the effects of a HD session on foveal thickness by optical coherence tomography (OCT) in adult patients with type 2 diabetes mellitus with chronic renal failure (CRF) secondary to stage 5 diabetic nephropathy in HD. PATIENTS AND METHODS: We studied 25 eyes of 14 patients who underwent analytical studies and pre-HD and post-HD OCT. RESULTS: As a group, the foveal thickness did not change after one session of HD in the 25 eyes studied (245.28 ± 52.21 µ versus 240.40 ± 40.25 µ) (p = 0.428) (2% reduction) or correlated with any clinical or laboratory parameters analyzed.When comparing the subgroup of 13 eyes in which the foveal thickness did not change or decreased compared to the subgroup of 12 eyes in which the foveal thickness increased we found that in the first subgroup the bath temperature was significantly higher (37.00 ± 0.00 versus 36.29 ° C, p = 0.008) and the conductivity was significantly lower (14.00 ± 0.00 versus 14.29 ± 0.10 mS / cm, p = 0.030). CONCLUSION: HD may modify the foveal retinal thickness as a function of changing parameters such as bath temperature and conductivity.