High pathogenic avian influenza A(H5) viruses of clade 2.3.4.4b in Europe-Why trends of virus evolution are more difficult to predict.

Since 2016, A(H5Nx) high pathogenic avian influenza (HPAI) virus of clade 2.3.4.4b has become one of the most serious global threats not only to wild and domestic birds, but also to public health. In recent years, important changes in the ecology, epidemiology, and evolution of this virus have been reported, with an unprecedented global diffusion and variety of affected birds and mammalian species. After the two consecutive and devastating epidemic waves in Europe in 2020-2021 and 2021-2022, with the second one recognized as one of the largest epidemics recorded so far, this clade has begun to circulate endemically in European wild bird populations. This study used the complete genomes of 1,956 European HPAI A(H5Nx) viruses to investigate the virus evolution during this varying epidemiological outline. We investigated the spatiotemporal patterns of A(H5Nx) virus diffusion to/from and within Europe during the 2020-2021 and 2021-2022 epidemic waves, providing evidence of ongoing changes in transmission dynamics and disease epidemiology. We demonstrated the high genetic diversity of the circulating viruses, which have undergone frequent reassortment events, providing for the first time a complete overview and a proposed nomenclature of the multiple genotypes circulating in Europe in 2020-2022. We described the emergence of a new genotype with gull adapted genes, which offered the virus the opportunity to occupy new ecological niches, driving the disease endemicity in the European wild bird population. The high propensity of the virus for reassortment, its jumps to a progressively wider number of host species, including mammals, and the rapid acquisition of adaptive mutations make the trend of virus evolution and spread difficult to predict in this unfailing evolving scenario.

Criminality rates in those sentenced for femicide in Mexico / Niveles de criminalidad en personas sentenciadas por el delito de feminicidio en México / Níveis de criminalidade em pessoas condenadas pelocrime de feminicídio no México

Introduction:Violence against women is a problem that affects the worldwide population, with its maximum expression being femicide. Due to the recent and unequal legislation regarding this phenomenon, recent studies have focused on identifying types of aggressors where criminal behavior plays an important role as a risk factor. Likewise, very few studies exist with people who have committed femicide. Objective:To determine the criminality rates in those sentenced for the crime of femicide. Materials and methods:A list with 20 dimensions was used to evaluate 62 people arrested for the crime of femicide. Results:4 levels of criminality were found, which can be classified from low to high; the principal indicators that marked differences between these levels where domestic violence and a history of alcohol and drug use. Conclusion:The indicators that marked the differences between the levels of criminality are a history of intimate partner violence used to resolve conflicts which is associated with use of alcohol and psychoactive drugs.

Introducción: La violencia en contra de la mujer es un problema que afecta a la población mundial, teniendo su máxima expresión a través del feminicidio. Debido a la reciente y desigual legislación de este fenómeno, estudios al respecto son recientes y han girado en torno a determinar tipologías de agresores donde la criminalidad juega un papel importante como factor de riesgo. Así mismo, existen muy pocos estudios directos con personas que han cometido feminicidio.Objetivo: Conocer el nivel de criminalidad en sentenciados por el delito de feminicidio. Materiales y métodos: A través de una lista con 20 dimensiones se evaluó a 62 personas privadas de su libertad por el delito de feminicidio.Resultados: Se encontraron cuatro niveles de criminalidad que van de baja a muy alta, los principales indicadores que marcan la diferencia entre estos niveles son los que se refieren a la violencia de pareja e historia de consumo de alcohol y drogas.Conclusión: Los indicadores que marcan la diferencia entre los niveles de criminalidad, se encuentran una historia de violencia hacia la pareja como una forma de resolver conflictos, asociada al consumo de alcohol y sustancias psicoactivas.

Introdução:A violência contra a mulher é um problema que afeta a população mundial, tendo sua expressão máxima por meio do feminicídio. Devido à recente e desigual legislação sobre o fenômeno, os estudos a respeito são recentes e têm girado em torno da determinação de tipos de agressores onde a criminalidade desempenha um papel importante como fator de risco. Da mesma forma, são poucos os estudos diretos com pessoas que cometeram feminicídio. Objetivo:Conhecer o nível de criminalidade em condenados pelo crime de feminicídio. Materiais e métodos:Através de uma lista com 20 dimensões, foram avaliadas 62 pessoas privadas de liberdade pelo crime de feminicídio. Resultados:Foram encontrados quatro níveis de criminalidade, que variam de baixo a muito alto, sendo que os principais indicadores que fazem a diferença entre esses níveis são aqueles relacionados à violência por parceiro íntimo e histórico de uso de álcool e drogas. Conclusão:A criminalidade surge como uma categoria de análise, que busca explicar algumas características nas pessoas que cometeram esse crime, porém, é preciso considerar outros fatores como cultura, estrutura social e características da população reclusa por feminicídio.

Influenza A(H5N1) detection in two asymptomatic poultry farm workers in Spain, September to October 2022: suspected environmental contamination.

In autumn 2022, the Spanish Influenza National Reference Laboratory (NRL) confirmed the detection of influenza A(H5N1) in samples from two asymptomatic workers linked to an outbreak in a poultry farm in Spain. Nasopharyngeal swabs were taken according to a national screening protocol for exposed workers. Absence of symptoms, low viral load and negative serology in both workers suggested environmental contamination. These findings motivated an update of the early detection strategy specifying timing and sampling conditions in asymptomatic exposed persons.

Highly pathogenic avian influenza A(H5N1) virus infection in farmed minks, Spain, October 2022.

In October 2022, an outbreak in Europe of highly pathogenic avian influenza (HPAI) A(H5N1) in intensively farmed minks occurred in northwest Spain. A single mink farm hosting more than 50,000 minks was involved. The identified viruses belong to clade 2.3.4.4b, which is responsible of the ongoing epizootic in Europe. An uncommon mutation (T271A) in the PB2 gene with potential public health implications was found. Our investigations indicate onward mink transmission of the virus may have occurred in the affected farm.

'If you aren't married yet, you'll be married to your treatment from now on': embodied mediations in a women's HIV peer advisory project in Mexico.

Treatment as Prevention is a key biopolitical intervention on the HIV epidemic but relies on individual adherence to antiretroviral treatment in order to have an effect on the population as a whole. Informed by a discussion of biopower, this paper analyses the complex relationships between discourses of competent authorities and modes of subjectification through a qualitative analysis of findings from 5 years of fieldwork associated with the action-research project Yantzin: Women HIV Peer Advisors in Mexico. It looks at the production of subjects of adherence, whereby peer advisors emerge as key agents at the interface between scientific and experiential knowledge. Contradictorily, the desire to live becomes feasible only by engaging with these biopolitical interventions. We discuss how peer advisors twist these technologies in such a way that they provide not only operations of power but also courses of action for desire. Through embodied mediation strategies that critique obedience to medical prescription and translate scientific information into bodily and emotionally shared experiences, peer advisors' work goes beyond the behavioural rationality of biomedical models offering embodied proof for other women that, even when living with HIV, a project of happiness is possible.

Comparison of 2016-17 and Previous Epizootics of Highly Pathogenic Avian Influenza H5 Guangdong Lineage in Europe.

We analyzed the highly pathogenic avian influenza (HPAI) H5 epizootic of 2016-17 in Europe by epidemiologic and genetic characteristics and compared it with 2 previous epizootics caused by the same H5 Guangdong lineage. The 2016-17 epizootic was the largest in Europe by number of countries and farms affected and greatest diversity of wild birds infected. We observed significant differences among the 3 epizootics regarding region affected, epidemic curve, seasonality, and outbreak duration, making it difficult to predict future HPAI epizootics. However, we know that in 2005-06 and 2016-17 the initial peak of wild bird detections preceded the peak of poultry outbreaks within Europe. Phylogenetic analysis of 2016-17 viruses indicates 2 main pathways into Europe. Our findings highlight the need for global surveillance of viral changes to inform disease preparedness, detection, and control.

Characterization of Eyeball Loss in Four Cities of Colombia.

PURPOSE: Describe the socio-demographic characteristics of anophthalmic patients examined at specialized centers of four cities in Colombia to know the different causes of eyeball loss. METHOD: A transversal retrospective study was done of 511 medical records from the specialized practices of four cities in Colombia. Socio-demographic data of patients who were seen between January 2011 and December 2013 were compiled. SOFA Statistics software v1.4.6 was used for this analysis. An analysis throughout the measures of central tendency for numerical variables was developed, and the descriptive statistics were used for the categorical variables. RESULTS: Almost 63% of the data belonged to male patients. Eyeball loss was more frequent in patients over 40 years of age. Fifty-one percent of the patients suffered eyeball loss due to traumatic causes, 40.2% due to pathological causes, and 4.6% due to congenital anomalies. The most frequent specific causes were glaucoma (19%), ocular cancer (15.4%), and home accidents (11,2%). Around 60% of the anophthalmic patients belonged to low socioeconomic level. CONCLUSIONS: It is important to highlight that more than half of the analyzed anophthalmia cases originated in some type of trauma; this means that they could be considered potentially avoidable losses. Complications deriving from glaucoma became the most frequent cause of anophthalmia in the pathological origin group, which suggests a reflection regarding the strategies of early detection of the disease and access to proper treatment. It is also showed the need to develop an efficient system to manage information.

Six-year surveillance of Newcastle disease virus in wild birds in north-eastern Spain (Catalonia).

Given that Newcastle disease (ND) is one of the major threats for the poultry industry, testing of Newcastle disease virus (NDV) has been carried out since 2010 in cases of mortality in wild birds (passive surveillance) in Catalonia. The objective is to provide an early warning system to prevent the infection of poultry. Since 2010, 35 episodes of mortality in wild birds were attributed to NDV infection. Throughout this period there was a progressive expansion of NDV to new areas, with an increase in the episodes of mortality, although it is not clear whether they were the result of the spread of the virus, or of the improvement of the surveillance. Phylogenetic analyses indicate that two distinct sublineages of NDV, 4a and 4b, were circulating in Catalonia. Both sublineages seem to be endemic in the wild bird population, affecting mainly Eurasian-collared doves, with a clear pattern in relation to its spatial distribution (coincident with the distribution of this species), and its temporal distribution (with the majority of cases between September and February). So far, endemicity in wild birds has not resulted in ND outbreaks in poultry. However, there are still many uncertainties about, for example, whether NDV may expand to new areas of Catalonia (with higher poultry density), or about the threat that the apparently more novel sublineage 4a may represent. Hence, efforts should be made so that measures to prevent infection of poultry farms (particularly in high-risk areas and periods) are encouraged, and surveillance is maintained.

Vaccination against H5 avian influenza virus induces long-term humoral immune responses in flamingoes (Phoenicopterus spp.).

Avian influenza (AI) can represent a threat to endangered wild birds, as demonstrated with the H5N1 highly pathogenic AI (HPAI) outbreaks. Vaccination against AI using inactivated H5-vaccines has been shown to induce humoral immune response in zoo bird species. In this study, the long-term efficacy of H5-vaccination was evaluated in flamingoes from Barcelona Zoo. Specific H5-antibody titres were maintained at high levels (geometric mean titres ≥32) for over 7 years after vaccination, both against the H5N9 and H5N3 vaccine strains, as well as H5N3 and H5N1 reference strains. In addition the breadth of the immune response was also studied by testing antibody production against H1-, H3-, H4-, H7-, and H10-subtypes. It was observed that most flamingoes presented specific antibodies against H1 virus subtypes, but titres to the other HA-subtypes were rarely detected. We show that AI-vaccines can induce immunity lasting seven years in flamingoes, which suggests that vaccination can provide long term protection from HPAI outbreaks in zoo birds.

Seroprevalence of paramyxoviruses in synanthropic and semi-free-range birds.

Avian paramyxoviruses (APMVs) are classified into nine different serotypes (APMV 1-9). Virulent strains of APMV-1 are already well characterized as the etiologic agent of Newcastle disease (ND), an important disease in poultry that is potentially capable of infecting all orders of avian species. However, very little is known about the other eight serotypes, the majority of which can cause disease in domestic birds. The role of synanthropic and semi-free-range birds as reservoirs of avian paramyxoviruses is not well understood and the main objective of this work was to evaluate the seroprevalence of APMV 1-9 in these kind of birds. A total of 296 sera, oropharyngeal swabs, and cloacal enemas were collected from semi-free-range birds belonging to four different species: feral pigeons (Columba livia var. domestica), hybrid ducks (Anas sp.), domestic geese (Anser anser domesticus), and white storks (Ciconia ciconia). Antibodies against NDV were found in 56.3% of domestic geese, 42.9% of feral pigeons, and 30.4% of hybrid ducks. Antibodies for other APMVs (-3, -4, -6, -7, -8, -9) were also found. Seven positive individuals were positive to real-time RT-PCR detection, all of them feral pigeons captured in 2006 and 2007. The results obtained reinforce the idea that semi-free-range birds may be good sentinels for the detection of NDV and other avian paramyxoviruses.

[Acceptability of male circumcision for HIV prevention among Mexican migrant men]. / Aceptabilidad de la circuncisión masculina para la prevención del VIH por parte de migrantes mexicanos.

OBJECTIVES: To explore cultural factors that influence the acceptability of male circumcision as a strategy for HIV prevention in migrant communities in Mexico. MATERIALS AND METHODS: Qualitative in-depth interviews with 20 men, 5 female partners of male migrants, and 5 health professionals in two rural communities in Michoacan. RESULTS: Although male immigrants would accept circumcision if recommended by a doctor, they know almost nothing about what the procedure involves, which generates fear and distrust. As a result, there is considerable distance between theoretically accepting the practice and actually becoming circumcised. Medical staff also expressed ambivalence about promoting male circumcision in rural communities in light of little knowledge about its effectiveness and concerns about informed consent. CONCLUSION: Based on the experiences of our interviewees, currently there are no cultural interpretations that would effectively facilitate the promotion of male circumcision for the prevention of HIV.

Aceptabilidad de la circuncisión masculina para la prevención del VIH por parte de migrantes mexicanos / Acceptability of male circumcision for HIV prevention among Mexican migrant men

Objetivos. Explorar factores culturales que inciden en la aceptabilidad de la circuncisión masculina como estrategia de prevención del VIH en comunidades de migrantes en México. Material y métodos. Estudio cualitativo mediante entrevistas a profundidad a 20 varones, a 5 mujeres parejas de migrantes y a 5 profesionistas de la salud, en dos comunidades rurales de Michoacán. Resultados. Aunque los varones aceptarían la circuncisión en caso de prescripción médica, existe un desconocimiento casi total del procedimiento, lo cual genera reacciones de temor y desconfianza. Esto sugiere una distancia entre aceptar y llevar a cabo dicho método. El personal médico consideró ambigua su promoción en comunidades rurales ante el desconocimiento sobre su efectividad y por falta de consentimiento informado. Conclusión. No existe antecedente cultural que pueda alojar la circuncisión masculina para prevención del VIH de manera positiva en la experiencia de los entrevistados.

Objectives. To explore cultural factors that influence the acceptability of male circumcision as a strategy for HIV prevention in migrant communities in Mexico. Materials and methods. Qualitative in-depth interviews with 20 men, 5 female partners of male migrants, and 5 health professionals in two rural communities in Michoacan. Results. Although male immigrants would accept circumcision if recommended by a doctor, they know almost nothing about what the procedure involves, which generates fear and distrust. As a result, there is considerable distance between theoretically accepting the practice and actually becoming circumcised. Medical staff also expressed ambivalence about promoting male circumcision in rural communities in light of little knowledge about its effectiveness and concerns about informed consent. Conclusion. Based on the experiences of our interviewees, currently there are no cultural interpretations that would effectively facilitate the promotion of male circumcision for the prevention of HIV.

Bagaza virus in partridges and pheasants, Spain, 2010.

In September 2010, an unusually high number of wild birds (partridges and pheasants) died in Cádiz in southwestern Spain. Reverse transcription PCR and virus isolation detected flavivirus infections. Complete nucleotide sequence analysis identified Bagaza virus, a flavivirus with a known distribution that includes sub-Saharan Africa and India, as the causative agent.

Comprehensive serological analysis of two successive heterologous vaccines against H5N1 avian influenza virus in exotic birds in zoos.

In 2005, European Commission directive 2005/744/EC allowed controlled vaccination against avian influenza (AI) virus of valuable avian species housed in zoos. In 2006, 15 Spanish zoos and wildlife centers began a vaccination program with a commercial inactivated H5N9 vaccine. Between November 2007 and May 2008, birds from 10 of these centers were vaccinated again with a commercial inactivated H5N3 vaccine. During these campaigns, pre- and postvaccination samples from different bird orders were taken to study the response against AI virus H5 vaccines. Sera prior to vaccinations with both vaccines were examined for the presence of total antibodies against influenza A nucleoprotein (NP) by a commercial competitive enzyme-linked immunosorbent assay (cELISA). Humoral responses to vaccination were evaluated using a hemagglutination inhibition (HI) assay. In some taxonomic orders, both vaccines elicited comparatively high titers of HI antibodies against H5. Interestingly, some orders, such as Psittaciformes, which did not develop HI antibodies to either vaccine formulation when used alone, triggered notable HI antibody production, albeit in low HI titers, when primed with H5N9 and during subsequent boosting with the H5N3 vaccine. Vaccination with successive heterologous vaccines may represent the best alternative to widely protect valuable and/or endangered bird species against highly pathogenic AI virus infection.

Influenza A virus subtypes in wild birds in North-Eastern Spain (Catalonia).

Since the spread of H5N1 highly pathogenic avian influenza virus in 2005, many surveillance programmes have been initiated in poultry and wild birds worldwide. This study describes for the first time the detection of different subtypes of avian influenza viruses (AIV) in wild birds in the West Mediterranean area (Catalonia, North-Eastern Spain). During a 3-year period (from mid-2006 to mid-2009), 1374 birds from 16 different families were examined, and a total of 62 AIV were detected by means of a real-time reverse transcriptase PCR assay. AIV were more frequently detected in Anatidae, Phoenicopteridae, Rallidae and Laridae families. Of the 62 positive samples, 28 AIV could be isolated in embryonated eggs. All isolates were subtyped by haemagglutinin and neuraminidase inhibition techniques and 10 different haemagglutinins (HA) and 7 neuraminidases (NA) were found in 13 different subtype combinations. The most common combinations were H4N6 (22.2%) and H1N1 (18.5%). The HA and NA gene sequences of different AIV subtypes were compared and aligned with those available AIV strains from genome databases. Our studies on AIV phylogenetic analysis revealed that all AIV genes sequenced from wild birds in North-Eastern Spain clustered within Eurasian avian clades, including the sequences of H8, N4 and N5 genes analyzed for the first time in Europe. The results contribute to the understanding of AIV in the Mediterranean area and in Europe.

West Nile virus in the endangered Spanish imperial eagle.

The Spanish imperial eagle (Aquila adalberti) is considered to be the most endangered European eagle. The species is an endemic resident in the Southwestern Iberian Peninsula. We used RT-PCR, immunohistochemistry and seroneutralization to test samples from 13 wild and 18 captive eagles. WNV was detected by RT-PCR in tissues and/or oropharyngeal swabs of eight of 10 (80%) imperial eagles analyzed, and both in apparently clinically healthy birds, and in animals that died due to secondary infections but had symptoms/lesions compatible with WNV. Immunohistochemistry detected WNV antigen in Purkinje cells in the cerebellum, epithelial cells of the gizzard and duodenum, perivascular inflammatory cells, and in Kupffer-cells and hepatocytes. Serum antibodies against WNV were detected in a total of five out of 21 imperial eagles (23.8%), including free-living nestlings (two out of nine samples, 22.2%) and captive adult eagles (three out of 12 samples, 25%). Our results evidence WNV circulation among free-living and captive Spanish imperial eagles in South-central Spain, a dry inland region with no previous WNV evidence, throughout 6 consecutive years. They also indicate the need for further research into this important zoonosis in order to better understand its epidemiology in the Mediterranean ecosystem and in order to understand the role of WNV in the population dynamics of the critically endangered Spanish imperial eagle.

A fully automated procedure for the high-throughput detection of avian influenza virus by real-time reverse transcription-polymerase chain reaction.

The recent spread of highly pathogenic H5N1 avian influenza (AI) has made it important to develop highly sensitive diagnostic systems for the rapid detection of AI genome and the differentiation of H5N1 variants in a high number of samples. In the present paper, we describe a high-throughput procedure that combines automated extraction, amplification, and detection of AI RNA, by an already described TaqMan real-time reverse transcription-polymerase chain reaction (RRT-PCR) assay targeted at the matrix (M) protein gene of AI virus (AIV). The method was tested in cloacal and tracheal swabs, the most common type of samples used in AI surveillance, as well as in tissue and fecal samples. A robotic system (QIAGEN Biosprint 96) extracted RNA and set up reactions for RRT-PCR in a 96-well format. The recovery of the extracted RNA was as efficient as that of a manual RNA extraction kit, and the sensitivity of the detection system was as high as with previously described nonautomated methods. A system with a basic configuration (one extraction robot plus two real-time 96-well thermocyclers) operated by two persons could account for about 360 samples in 5 hr. Further characterization of AI RNA-positive samples with a TaqMan RRT-PCR specific for H5 (also described here) and/or N1 was possible within 2 hr more. As this work shows, the system can analyze up to 1400 samples per working day by using two nucleic acid extraction robots and a 384-well-format thermocycler.

A real-time TaqMan RT-PCR method for neuraminidase type 1 (N1) gene detection of H5N1 Eurasian strains of avian influenza virus.

This work describes the development of a real-time RT-PCR (RRT-PCR) procedure for detection of the N1 gene from avian influenza virus (AIV), based on the use of specific primers and a TaqMan-MGB (minor groove binder) probe. Nucleotide sequences of the neuraminidase type 1 gene from a collection of H5N1 Eurasian strains of AIV were aligned using ClustalW software. Conserved regions were located and used to design specific primers and a TaqMan-MGB probe using Primer Express software. A one-step RRT-PCR method was optimized using RNA from the Turkey 2005 H5N1 strain of AIV and can be completed in about 2 hr once the RNA is extracted from the sample. The specificity of the assay was assessed with non-N1 AIV strains, another related avian virus, and different avian tissue samples from healthy animals. Sensitivity was determined using 10-fold serial dilutions of the H5N1 Turkey 2005 strain and was compared with the generic RRT-PCR detection method, targeted at the matrix protein gene of AIV, commonly used at the Spanish AIV National Reference Laboratory. The N1 detection method proved to be even more sensitive than the generic (matrix-based) method, allowing a very quick confirmation (or discarding) of any Eurasian N1 strain when a positive result was obtained with the matrix RRT-PCR assay. Combined with RRT-PCR tests for general detection of AIV and H5 typing in use at the NRL, the procedure here described allows characterizing of any H5N1 Eurasian AIV strain in a field sample within a working day.