ABSTRACT
Introducción y objetivos. La inmunoterapia oral (ITO) es una alternativa a la dieta de evitación en algunas alergias alimentarias. El objetivo de este trabajo es evaluar la eficacia y seguridad de la ITO con huevo en una consulta de alergia pediátrica. Material y métodos. Estudio observacional, longitudinal y retrospectivo de pacientes pediátricos con alergia al huevo persistente sometidos a ITO. Para la inducción se utilizó proteína de clara de huevo deshidratada administrada diariamente y con incrementos semanales hasta alcanzar una dosis de 4 gramos. Para la fase de mantenimiento se indicó una ingesta de al menos dos o tres huevos a la semana. Resultados. Se trataron 14 pacientes (6 niñas), de entre 5 y 13 años (mediana 5,5 años). Se consiguió desensibilización completa al final de la inducción en 11 pacientes (78,6%), que se mantuvo en todos ellos tras una mediana de tiempo de seguimiento de 29 meses. Durante la inducción los síntomas más frecuentes fueron: prurito orofaríngeo (9/14), dolor abdominal (7/14) y rinoconjuntivitis (6/14). Se emplearon antihistamínicos en 8 casos (57,1%) y ninguno precisó adrenalina. Entre los pacientes que consiguieron desensibilización se observó una tendencia al descenso de las IgE específicas, siendo estadísticamente significativo para las IgE a huevo completo (p = 0,047), clara de huevo (p = 0,031) y ovoalbúmina (p = 0,016). Conclusiones. La ITO con clara de huevo deshidratada resultó ser un tratamiento muy eficaz y bien tolerado en población pediátrica con alergia al huevo (AU)
Background and objective. Oral immunotherapy (OIT) is an alternative to strict avoidance for the management of some food allergies. The aim of this study is to assess the efficacy and safety of egg OIT in a paediatric allergy outpatient service. Methods. Retrospective, longitudinal observational study in children with persistent hen egg allergy who received egg OIT. For the build-up phase, dehydrated egg white was used daily. Updosing was performed weekly at the allergy unit, up to a final dose of 4 grams. Maintenance phase was carried out with a daily intake of one egg at least two or three times a week. Results. 14 patients (6 girls), whose ages ranged from 5 to 13 years (median 5.5 years) were treated with egg OIT. Eleven subject (78.6%) reached total desensitization, and all of them remained desensitized after a median follow-up time of 29 months. The most frequent adverse effects detected during the build-up phase were: oropharyngeal pruritus (9/14), abdominal pain (7/14), and rhinoconjuntivitis (6/14). Eight patients (57.1%) required oral antihistamines, and none received adrenaline. In those subjects that reached total desensitization, a trend to lower specific IgE levels was observed. That trends were statistically significand for whole egg (p = 0.047), egg white (p = 0.031), and ovalbumin (p = 0.016). Conclusions. Egg OIT was an effective and well tolerated treatment in children with egg allergY (AU)
Subject(s)
Humans , Male , Female , Child, Preschool , Child , Adolescent , Egg Hypersensitivity/therapy , Desensitization, Immunologic , Immunotherapy/methods , Longitudinal Studies , Retrospective Studies , Treatment Outcome , Administration, OralABSTRACT
IMPORTANCE: Altough disease-modifying factors such as malnutrition and diet have been associated with Alzheimer's disease (AD), little is known about the effects of pharmacological therapies on the nutritional status of AD patients. OBJECTIVE: To evaluate the nutritional status, prealbumin, and albumin serum levels and several anthropometric measurements in patients with probable moderate-stage AD, with and without rivastigmine drug treatment. STUDY DESIGN: A cross-sectional study. SETTING AND PARTICIPANTS: 34 patients were included, 17 with rivastigmine treatment and 17 without pharmacological treatment, over 60 years of both sexes. MEASUREMENTS: The nutritional status was evaluated using the Mini Nutritional Assessment (MNA). Albumin and prealbumin (transthyretin) levels and anthropometric evaluation were assessed using standard methods. RESULTS: A polarity of malnutrition was detected in the untreated group. According to the MNA survey, the risk of malnutrition is higher without rivastigmine treatment (p = 0.0001). There are a less loss of appetite, less psychological stress, greater mobility and independence in those patients receiving rivastigmine (p = 0.003, 0.008, 0.016 and 0.018, respectively). The body mass index does not show a statistical difference, however, categorizing it for older adults, this index was improved in those receiving rivastigmine (p = 0.016). The serum levels of albumin and prealbumin showed no significant statistical difference between the groups. CONCLUSION: Rivastigmine treatment shows a protective effect on malnutrition in patients with moderate-stage AD.
Subject(s)
Alzheimer Disease/physiopathology , Cholinesterase Inhibitors/therapeutic use , Malnutrition/complications , Nutrition Assessment , Nutritional Status/physiology , Rivastigmine/therapeutic use , Aged , Cholinesterase Inhibitors/pharmacology , Cross-Sectional Studies , Female , Humans , Male , Rivastigmine/pharmacologyABSTRACT
The SEOM/GEINO clinical guidelines provide recommendations for radiological, and molecular diagnosis, treatment and follow-up of adult patients with anaplastic gliomas (AG). We followed the 2016 WHO classification which specifies the major diagnostic/prognostic and predictive value of IDH1/IDH2 missense mutations and 1p/19q codeletions in AG. The diagnosis of anaplastic oligoastrocytoma is discouraged. Surgery, radiotherapy and chemotherapy with PCV or TMZ are the first-line standard of care for AG with slight modifications according to molecular variables. A multidisciplinary team is highly recommended in the management of these tumors.
ABSTRACT
The SEOM/GEINO clinical guidelines provide recommendations for radiological, and molecular diagnosis, treatment and follow-up of adult patients with anaplastic gliomas (AG). We followed the 2016 WHO classification which specifies the major diagnostic/prognostic and predictive value of IDH1/IDH2 missense mutations and 1p/19q codeletions in AG. The diagnosis of anaplastic oligoastrocytoma is discouraged. Surgery, radiotherapy and chemotherapy with PCV or TMZ are the first-line standard of care for AG with slight modifications according to molecular variables. A multidisciplinary team is highly recommended in the management of these tumors.
Subject(s)
Brain Neoplasms/diagnosis , Brain Neoplasms/therapy , Glioma/diagnosis , Glioma/therapy , HumansABSTRACT
La investigación se ejecutó en la Finca Experimental "La María" propiedad de la Universidad Técnica Estatal de Quevedo (UTEQ) localizada en el km 7¹/2 de la vía Quevedo-Mocache; Provincia de Los Ríos cuya ubicación geográfica de 1º 6' 23" de latitud sur y 79º 29' 12" de longitud oeste y a una altura de 73 m.s.n.m. El objetivo principal fue evaluar el comportamiento productivo de cuyes con la inclusión del 20% de harinas derivadas de follajes arbustivos y arbóreos tropicales. Se utilizaron 40 cuyes macho de 30 días de edad. Se empleó un diseño completamente al azar con cinco tratamientos, cuatro réplicas y la unidad experimental estuvo conformado por dos cuyes. Para determinar las diferencias entre medias de tratamientos se aplicó la prueba de Tukey (P≤0.05). Se evaluaron cinco dietas-tratamientos: (T0) dieta 100% balanceado, (T1) 80% dieta y 20% harina de Morus alba, (T2) 80% dieta y 20% harina de Erythrina poeppigiana, (T3) 80% dieta y 20% harina de Tithonia diversifolia, (T4) 80% dieta y 20% harina de Hibiscus rosa-sinensis. Las variables bajo estudio fueron: consumo de alimento de balanceado en materia seca (CABMS, g), ganancia de peso (GP, g), índice de conversión alimenticia (ICA) y rendimiento en canal (RC, %). La rentabilidad de los tratamientos se determinó a través de la relación beneficio-costo (R b/c). Los mayores (P<0.01) CABMS, GP-ICA y RC-Rentabilidad, la registraron los tratamientos: T0 (48.34 g MS animal-1 d-1), T1 (8.80 g animal-1 d-1 y 5.04) y el T3 (77.67% y 26.20%), respectivamente.
The research was carried out at the Experimental farm "La María" property of the State Technical University of Quevedo located at km 7¹/2 in road Quevedo-Mocache; Los Ríos province, with a geographical location of 1° 6' 23" south latitude and 79º 29' 12" west longitude, at 73 meters altitude. The aim was to evaluate the productive effect of leaf meal and tropical shrubs with inclusion 20%. Were used 40 male guinea pigs of 30 days age and a completely randomized design with five treatments and four repetitions, two male guinea pigs was used to study. A 56-days experiment was conducted, and was applied the Tukey test (P≤0.05) to determine differences. Five treatments in diets were evaluated: (T0) 100% balanced diet; (T1) 80% and 20% leaf meal Morus alba flour (T2); 80% diet and 20% Erythrina poeppigiana flour; (T3) 80% diet and 20% Tithonia diversifolia flour; (T4) 80% diet and 20% Hibiscus rosa-sinensis flour. The following variables were used: Balanced feed consumption in dry matter (CABMS, g), weight obtained (GP, g), alimentary conversion index (ICA), performance distribution channel (RC, %). The profitability of the treatments was determined using the benefit-cost ratio (R b/c) ratio. The higher (P<0.01) CABMS, GP-ICA, RC- yield, assigned treatments: T0 (48.34 g DM animal-1 d-1), T1 (8.80 g animal-1 d-1 and 5.04) and T3 (77.67 % and 26.20%), respectively.
ABSTRACT
Schistosomiasis is a major public health concern, with 200 million people infected worldwide. In Brazil, this disease has been reported in 19 states, and its prevalence in the city of Barra Mansa in Rio de Janeiro State is 1 %. The parasitological diagnostic methods currently available in these areas lack sensitivity; however, enzyme-linked immunosorbent assays (ELISAs) have been employed successfully for the diagnosis of schistosomiasis by using antibodies against antigens of Schistosoma mansoni adult worms and eggs, and for the detection of circulating antigens. The objective of this study was to determine systematically the prevalence of S. mansoni infection in the peripheral areas of Barra Mansa. A cross-sectional study was conducted from April to December 2011 by using probabilistic sampling that collected 610 fecal samples and 612 serum samples. ELISA-IgG with total extracts and ELISA-IgM with trichloroacetic acid-soluble fractions were employed to detect antibodies against S. mansoni and were compared with the Kato-Katz and Hoffman parasitological techniques. Among the individuals studied, anti-S. mansoni antibodies were detected in 11.16 % (n = 71) by ELISA-IgG and in 20.75 % (n = 132) by ELISA-IgM, while the parasitological techniques showed 0.82 % (n = 5) positivity. The agreement between the two ELISA tests was 85.38 % (n = 543), and 8.65 % (n = 55) of the serum samples showed positive results in both tests. The higher positivity of the ELISA-IgM test corroborates the results of previous reports and indicates that the test may be a useful tool in epidemiological studies, particularly in areas of low endemicity for S. mansoni.
Subject(s)
Antibodies, Helminth/blood , Enzyme-Linked Immunosorbent Assay/methods , Immunoglobulin G/blood , Immunoglobulin M/blood , Schistosoma mansoni/isolation & purification , Schistosomiasis mansoni/diagnosis , Adolescent , Adult , Animals , Brazil/epidemiology , Child , Child, Preschool , Cricetinae , Cross-Sectional Studies , Feces , Female , Humans , Infant , Male , Middle Aged , Prevalence , Schistosoma mansoni/immunology , Schistosomiasis mansoni/blood , Schistosomiasis mansoni/epidemiology , Schistosomiasis mansoni/immunology , Young AdultABSTRACT
OBJETIVO: En esta investigación se evaluó la infiltración anestésica intracervical en pacientes en situación de aborto para realizar el legrado uterino instrumental. Pacientes y métodos Se estudió una muestra de 20 pacientes en situación de aborto. El estudio fue abierto, prospectivo y exploratorio. La edad de las pacientes tuvo un rango de 17 a 49 años de edad, con una media y desviación estándar de 26,95 ± 9,2058. La edad de la gestación tuvo un rango de 5 a 14 semanas con una media y desviación estándar de 8,75 ± 2,4622. Para el análisis estadístico se usó el programa SPSS. Para la anestesia intracervical se utilizó lidocaína al 1%, 10 ml; 5 ml en cada una de las infiltraciones, la primera a las III y la segunda a las IX de las manecillas del reloj. RESULTADOS: La duración de la anestesia tuvo un rango de 30 a 70 min, con una media y desviación estándar de 48,25 ± 13,8992. CONCLUSIÓN: La duración del tiempo quirúrgico desde la infiltración anestésica y el legrado uterino tuvieron un rango de 7 a 14min, con una media y desviación estándar de 10,9 ± 2,1886. El sangrado tuvo un rango de 50 a 150ml, con una media y desviación estándar de 100 ± 44,7213. Todos los estudios fueron longitudinales
Objetive: We performed an open, prospective, exploratory and longitudinal study to evaluate the use of intracervical anesthetic infiltration with instrumental uterine curettage in 20 women undergoing pregnancy termination. PATIENTS AND METHODS: The patients' age ranged from 17 to 49 years (mean and SD:26.95±9.2058). The length of gestation ranged from 5 to 14 weeks (mean and SD:8.75±2.4622). The SPSS program was used for the statistical analysis. RESULTS: For intracervical anesthesia, 10 ml lidocaine at 1% was used; 5ml was applied at infiltration points III and IX clockwise. Anesthesia lasted 30 to 70 minutes (mean and SD: 48.25 ± 13.8992). CONCLUSIONS: Operating time (infiltration and curettage) ranged from 7 to 14 minutes (mean and SD: 10.9 ± 2.1886). Blood loss ranged from 50 to 150ml (mean and SD: 100 ± 44.7213). All studies were longitudinal
Subject(s)
Humans , Female , Dilatation and Curettage/methods , Anesthesia, Local/methods , Abortion , Prospective Studies , Blood Loss, SurgicalABSTRACT
OBJECTIVE: To compare transfusion requirements and erythropoietic response in preterms between schedules of rEPO administration once or three times per week, using the same weekly dose. STUDY DESIGN: Prospective, randomized trial including infants weighing <1500 g at birth and/or were îº32 weeks' gestation: Group 1 (60 infants) received subcutaneous rEPO at 250 units kg(-1) per dose, three times weekly for 6 weeks; Group 2 (59 infants), at 750 units kg(-1) per dose, once weekly for 6 weeks. Efficacy was evaluated based on the transfusion requirement, hemoglobin changes, reticulocyte counts, serum transferrin receptor (sTfR) and serum ferritin. The frequency of adverse effects was registered in both groups. RESULT: A total of 13 infants were transfused in each group (relative risk: 0.98; 95% confidence interval: 0.4 to 2.3). Phlebotomy loss and red blood cell transfusion volumes received were similar in both groups. Hemoglobin levels were lower at end of study in Group 2 (10.6±1.5 g dl(-1) versus 11.5±1.4 g dl(-1); P<0.003). At end of study, reticulocyte counts and sTfR values increased and serum ferritin values decreased, without significant differences between the two groups. Incidence of complications was similar in both groups. CONCLUSION: The once-weekly rEPO schedule for very low birth weight infants proved as effective as the three-times-weekly schedule, in relation to erythropoietic stimulus and transfusion requirement.
Subject(s)
Anemia, Neonatal , Blood Transfusion/statistics & numerical data , Erythropoiesis/drug effects , Erythropoietin , Infant, Premature, Diseases , Anemia, Neonatal/metabolism , Anemia, Neonatal/physiopathology , Anemia, Neonatal/therapy , Drug Administration Schedule , Drug Monitoring , Erythropoietin/administration & dosage , Erythropoietin/adverse effects , Ferritins/blood , Hemoglobins/analysis , Humans , Infant, Newborn , Infant, Premature , Infant, Premature, Diseases/metabolism , Infant, Premature, Diseases/physiopathology , Infant, Premature, Diseases/therapy , Infant, Very Low Birth Weight , Injections, Subcutaneous , Receptors, Transferrin/blood , Recombinant Proteins , Reticulocyte Count , Thrombocytosis/etiology , Transfusion Reaction , Treatment OutcomeABSTRACT
Toxoplasmosis is an infection caused by Toxoplasma gondii, an intracellular obligate parasite. Its transmission has usually been attributed to ingestion of undercooked or raw meat. The frequency of T. gondii in pork, the most common meat for human consumption in Jalisco, Mexico, is unknown; in Guadalajara city high prevalence of human toxoplasmosis has been documented. Forty-eight samples of pork meat from butcher shops in Ocotlán city were analyzed. Through bioassay, 50 g of tissue was homogenized in an acidic pepsin solution and inoculated subcutaneously to previously immunosuppressed mice. Blood samples from the mice tail vein were obtained before inoculation and 7, 14, 28, and 45 days postinoculation to analyze anti-Toxoplasma immunoglobulin (Ig) M and IgG antibody kinetics by indirect enzyme-linked immunosorbent assay. For histopathology, small fragments of the brain, lungs, heart, and skeletal muscle were extracted on day 45 and were stained with hematoxylin and eosin. Also, DNA was extracted from the pork meat for PCR amplification of the B1 gene. Even though all pork samples were negative by histopathology and PCR, IgG and IgM antibodies against T. gondii were detected in 1 of the 48 inoculated mice, reflecting a frequency of 2.1% positive pork meat, which is lower than expected but similar to that found in other regions.
Subject(s)
Food Contamination/analysis , Food Parasitology , Meat/parasitology , Toxoplasma/isolation & purification , Animals , Antibodies, Protozoan/blood , Biological Assay , Humans , Immunoglobulin G/blood , Immunoglobulin M/blood , Mexico/epidemiology , Mice , Prevalence , Swine , Toxoplasma/immunology , Toxoplasmosis/prevention & control , Toxoplasmosis/transmission , Toxoplasmosis, Animal/immunology , Toxoplasmosis, Animal/parasitology , Toxoplasmosis, Animal/pathologyABSTRACT
The mechanisms by which alcohol exacerbates liver injury in patients with hepatitis C are unknown. We used the hepatitis C virus (HCV) subgenomic replicon cell system to evaluate the effect of ethanol on HCV replication and viral protein synthesis. Our results demonstrate that alcohol stimulates HCV replicon expression at both HCV-RNA and protein levels. Furthermore, we observed that ethanol treatment showed an additive effect in cyclooxygenase-2 (COX-2) protein expression and activity already induced by HCV viral proteins, and in turn increased HCV viral expression. Our results suggest that COX-2 activity is involved in ethanol-induced HCV-RNA and NS5A protein expression, because acetylsalicylic acid (ASA), a COX-1/2 inhibitor, blocked this induction and downregulated COX-2 protein expression and activity. Therefore, we suggest that ethanol increases HCV replication expression, at least in part, by upregulating a key cellular regulator of oxidative stress pathway known as COX-2 or its products.
Subject(s)
Cyclooxygenase 2/metabolism , Ethanol/toxicity , Hepacivirus/physiology , Hepatitis C/virology , Replicon , Cell Line , Dinoprostone/analysis , Hepacivirus/drug effects , Humans , Liver , Luciferases/metabolism , Viral Nonstructural Proteins/metabolism , Virus ReplicationABSTRACT
Schwann cell (SC) implantation alone has been shown to promote the growth of propriospinal and sensory axons, but not long-tract descending axons, after thoracic spinal cord injury (SCI). In the current study, we examined if an axotomy close to the cell body of origin (so as to enhance the intrinsic growth response) could permit supraspinal axons to grow onto SC grafts. Adult female Fischer rats received a severe (C5) cervical contusion (1.1 mm displacement, 3 KDyn). At 1 week postinjury, 2 million SCs ex vivo transduced with lentiviral vector encoding enhanced green fluorescent protein (EGFP) were implanted within media into the injury epicenter; injury-only animals served as controls. Animals were tested weekly using the BBB score for 7 weeks postimplantation and received at end point tests for upper body strength: self-supported forelimb hanging, forearm grip force, and the incline plane. Following behavioral assessment, animals were anterogradely traced bilaterally from the reticular formation using BDA-Texas Red. Stereological quantification revealed a twofold increase in the numbers of preserved NeuN+ neurons rostral and caudal to the injury/graft site in SC implanted animals, corroborating previous reports of their neuroprotective efficacy. Examination of labeled reticulospinal axon growth revealed that while rarely an axon was present within the lesion site of injury-only controls, numerous reticulospinal axons had penetrated the SC implant/lesion milieu. This has not been observed following implantation of SCs alone into the injured thoracic spinal cord. Significant behavioral improvements over injury-only controls in upper limb strength, including an enhanced grip strength (a 296% increase) and an increased self-supported forelimb hanging, accompanied SC-mediated neuroprotection and reticulospinal axon growth. The current study further supports the neuroprotective efficacy of SC implants after SCI and demonstrates that SCs alone are capable of supporting modest supraspinal axon growth when the site of axon injury is closer to the cell body of the axotomized neuron.
Subject(s)
Axons/physiology , Efferent Pathways/physiology , Forelimb/physiology , Muscle Strength/physiology , Schwann Cells/transplantation , Spinal Cord Compression , Animals , Axotomy , Behavior, Animal/physiology , Cells, Cultured , Female , Green Fluorescent Proteins/genetics , Green Fluorescent Proteins/metabolism , Hand Strength , Rats , Rats, Inbred F344 , Schwann Cells/cytology , Schwann Cells/physiology , Spinal Cord/cytology , Spinal Cord/metabolism , Spinal Cord/pathology , Spinal Cord Compression/pathology , Spinal Cord Compression/therapyABSTRACT
BACKGROUND: Hyperhomocysteinaemia is a factor related to the development of atherosclerosis in rheumatoid arthritis (RA). However, Hispanics with RA develop high rates of coronary disease; there are no studies about the frequency and factors related to high levels of homocysteine in Mexican patients. OBJECTIVE: To evaluate the prevalence and characteristics associated with hyperhomocysteinaemia in Mexican patients with RA. METHODS: One hundred and fifty-two patients with RA were compared with 153 controls. The assessment in RA included clinical characteristics, disease activity (RADAR), functioning (HAQ-Di and global functional status), comorbidity, and radiological damage. Laboratory determinations included total serum homocysteine (tHcy), erythrocyte sedimentation rate (ESR), C-reactive protein (CRP), rheumatoid factor (RF), and lipid profile. RESULTS: Median levels of homocysteine were higher in RA compared with controls (11.3 vs. 9.3, p<0.001). Twenty per cent of the patients with RA had hyperhomocysteinaemia (>15 micromol/L) compared with 6% in controls (p<0.001). There was statistical association between hyperhomocysteinaemia in RA with male gender (p<0.001), impairment in the global functional status (p = 0.004), higher radiological damage (p = 0.001), and CRP (p = 0.04). There was no association with RADAR, HAQ-Di, or RF, methotrexate dose or duration of use. In the adjusted multivariate model, the two variables associated with higher risk for hyperhomocysteinaemia were male gender (OR = 4.2, 95% CI 2 to 12, p = 0.006) and higher radiological damage (III-IV) (OR = 3.4, 95% CI 1.3 to 9, p = 0.01). CONCLUSIONS: Our data show a high prevalence of hyperhomocysteinaemia in Mexican patients with RA. More effort is required to evaluate and treat earlier this coronary risk factor.
Subject(s)
Arthritis, Rheumatoid/complications , Homocysteine/blood , Hyperhomocysteinemia/epidemiology , Adolescent , Adult , Aged , Arthritis, Rheumatoid/blood , Arthritis, Rheumatoid/epidemiology , Atherosclerosis/epidemiology , Atherosclerosis/etiology , Biomarkers/blood , C-Reactive Protein/metabolism , Cholesterol/blood , Female , Follow-Up Studies , Humans , Hyperhomocysteinemia/blood , Hyperhomocysteinemia/complications , Incidence , Male , Mexico/epidemiology , Middle Aged , Retrospective Studies , Rheumatoid Factor/blood , Risk Factors , Severity of Illness IndexABSTRACT
Due to an ever-growing population of individuals with chronic spinal cord injury, there is a need for experimental models to translate efficacious regenerative and reparative acute therapies to chronic injury application. The present study assessed the ability of fluid grafts of either Schwann cells (SCs) or olfactory ensheathing glia (OEG) to facilitate the growth of supraspinal and afferent axons and promote restitution of hind limb function after transplantation into a 2-month-old, moderate, thoracic (T8) contusion in the rat. The use of cultured glial cells, transduced with lentiviral vectors encoding enhanced green fluorescent protein (EGFP), permitted long-term tracking of the cells following spinal cord transplantation to examine their survival, migration, and axonal association. At 3 months following grafting of 2 million SCs or OEG in 6 microl of DMEM/F12 medium into the injury site, stereological quantification of the three-dimensional reconstructed spinal cords revealed that an average of 17.1 +/- 6.8% of the SCs and 2.3 +/- 1.4% of the OEG survived from the number transplanted. In the OEG grafted spinal cord, a limited number of glia were unable to prevent central cavitation and were found in patches around the cavity rim. The transplanted SCs, however, formed a substantive graft within the injury site capable of supporting the ingrowth of numerous, densely packed neurofilament-positive axons. The SC grafts were able to support growth of both ascending calcitonin gene-related peptide (CGRP)-positive and supraspinal serotonergic axons and, although no biotinylated dextran amine (BDA)-traced corticospinal axons were present within the center of the grafts, the SC transplants significantly increased corticospinal axon numbers immediately rostral to the injury-graft site compared with injury-only controls. Moreover, SC grafted animals demonstrated modest, though significant, improvements in open field locomotion and exhibited less foot position errors (base of support and foot rotation). Whereas these results demonstrate that SC grafts survive, support axon growth, and can improve functional outcome after chronic contusive spinal cord injury, further development of OEG grafting procedures in this model and putative combination strategies with SC grafts need to be further explored to produce substantial improvements in axon growth and function.
Subject(s)
Axons/physiology , Graft Survival , Neuroglia/transplantation , Schwann Cells/transplantation , Spinal Cord Injuries/therapy , Animals , Cell Survival , Chronic Disease , Female , Genetic Vectors , Green Fluorescent Proteins/genetics , Hindlimb/physiology , Lentivirus/genetics , Nerve Regeneration/physiology , Neuroglia/cytology , Neuroglia/physiology , Olfactory Mucosa/cytology , Phenotype , Rats , Rats, Inbred F344 , Recovery of Function , Schwann Cells/cytologyABSTRACT
Cervical contusive trauma accounts for the majority, of human spinal cord injury (SCI), yet experimental use of cervical contusion injury models has been limited. Considering that (1) the different ways of injuring the spinal cord (compression, contusion, and transection) induce very different processes of tissue damage and (2) the architecture of the spinal cord is not uniform, it is important to use a model that is more clinically applicable to human SCI. Therefore, in the current study we have developed a rat model of contusive, cervical SCI using the Electromagnetic Spinal Cord Injury Device (ESCID) developed at Ohio State University (OSU) to induce injury by spinal cord displacement. We used the device to perform mild, moderate and severe injuries (0.80, 0.95, and 1.1 mm displacements, respectively) with a single, brief displacement of <20 msec upon the exposed dorsal surface of the C5 cervical spinal cord of female (180-200 g) Fischer rats. Characterization of the model involved the analysis of the temporal histopathological progression of the injury over 9 weeks using histochemical stains to analyze white and gray mater integrity and immunohistochemistry to examine cellular changes and physiological responses within the injured spinal cord. Accompanying the histological analysis was a comprehensive determination of the behavioral functionality of the animals using a battery of motor tests. Characterization of this novel model is presented to enable and encourage its future use in the design and experimental testing of therapeutic strategies that may be used for human SCI.
Subject(s)
Nerve Degeneration/pathology , Neurons/pathology , Spinal Cord Injuries/pathology , Spinal Cord/pathology , Animals , Anterior Horn Cells/pathology , Disease Models, Animal , Disease Progression , Female , Movement Disorders/diagnosis , Movement Disorders/etiology , Movement Disorders/pathology , Nerve Degeneration/physiopathology , Nerve Fibers, Myelinated/pathology , Nerve Regeneration/physiology , Neural Pathways/injuries , Neural Pathways/pathology , Neural Pathways/physiopathology , Neurosurgical Procedures/instrumentation , Neurosurgical Procedures/methods , Paresis/diagnosis , Paresis/etiology , Paresis/pathology , Posterior Horn Cells/pathology , Rats , Rats, Inbred F344 , Recovery of Function/physiology , Spinal Cord/physiopathology , Spinal Cord Injuries/physiopathology , Time FactorsABSTRACT
UNLABELLED: Nitric oxide (NO) has been implicated in cirrhosis and might be implicated in renal failure end-stage cirrhosis. AIM: Our aim was to evaluate NO role in renal failure induced during decompensated cirrhosis, using the following inhibitors: aminoguanidine (AG), a specific inducible nitric oxide synthase (iNOS) inhibitor and NG-nitro-l-arginine methyl ester (L-NAME), a nonselective blocker of NOS isoforms. METHODS: Endothelial (eNOS) and iNOS gene expression was analyzed by reverse transcriptase-polymerase chain reaction. Cirrhotic rats received a single intragastric dose of CCl(4) to induce acute liver damage (ALD). RESULTS: After ALD, aspartate aminotransferase highest levels were observed in rats treated with AG and ALT in rats treated with L-NAME. Inhibitors decreased creatinine serum levels to normal values and serum sodium levels re-established after the third day of ALD. L-NAME diminished (P<0.05) eNOS RNA renal expression. Renal iNOS with no inhibitor was overexpressed but was down-regulated by AG treatment. Liver eNOS RNA expression had a decreased expression before ALD in cirrhotic rats, but L-NAME treatment down-regulated eNOS after ALD. AG induced an important iNOS liver decrease. CONCLUSION: Both inhibitors improved renal function, although AG displayed a better effect and did not aggravate liver function. We concluded that NOS isoforms are implicated in the renal pathophysiologic events induced by ALD.
Subject(s)
Enzyme Inhibitors/therapeutic use , Guanidines/therapeutic use , Liver Cirrhosis, Experimental/drug therapy , NG-Nitroarginine Methyl Ester/therapeutic use , Nitric Oxide Synthase/antagonists & inhibitors , Renal Insufficiency/drug therapy , Animals , Aspartate Aminotransferases/blood , Creatinine/blood , Disease Models, Animal , Kidney/drug effects , Kidney/enzymology , Kidney/pathology , Liver/drug effects , Liver/enzymology , Liver/pathology , Liver Cirrhosis, Experimental/blood , Liver Cirrhosis, Experimental/enzymology , Liver Cirrhosis, Experimental/pathology , Male , Nitric Oxide Synthase/genetics , Nitric Oxide Synthase/metabolism , Nitric Oxide Synthase Type II , Nitric Oxide Synthase Type III , RNA, Messenger/genetics , RNA, Messenger/metabolism , Rats , Rats, Wistar , Renal Insufficiency/blood , Renal Insufficiency/enzymology , Renal Insufficiency/pathology , Sodium/bloodABSTRACT
We describe a 25-year-old Caucasian man with a 13-year history of inflammatory Crohn's disease (CD) who was suffering recurrent severe oral and esophageal ulcerations for the past 3 years. His CD had been treated with infliximab infusions among other medications. The loss of efficacy was confirmed by antibodies to infliximab (ATI) and serum infliximab tests that showed high levels of ATIs and undetectable levels of infliximab respectively. These findings were consistent with significant immunogenic response to infliximab leading to loss of effect. Infliximab infusions and prednisone were discontinued and treatment of the CD was instituted with adalimumab, a human anti-tumor necrosis factor (TNF)-alpha biologic agent, to control the inflammatory small intestinal disease and dapsone for the oral and esophageal CD ulcerations. The patient's oral and esophageal lesions as well as the enteric CD are under control after 5 months of therapy.
Subject(s)
Antibodies, Monoclonal/therapeutic use , Crohn Disease/drug therapy , Dermatologic Agents/therapeutic use , Esophageal Diseases/etiology , Oral Ulcer/drug therapy , Adalimumab , Adult , Antibodies, Monoclonal/immunology , Antibodies, Monoclonal, Humanized , Crohn Disease/complications , Dermatologic Agents/immunology , Drug Tolerance , Esophageal Diseases/drug therapy , Humans , Infliximab , Male , Oral Ulcer/etiology , Ulcer/drug therapy , Ulcer/etiologyABSTRACT
A 40-year-old woman underwent cesarean section under epidural anesthesia. The anesthetic procedure was carried out in strict aseptic conditions, the catheter was withdrawn 24 hours after surgery, and the patient was discharged 5 days after surgery. She was readmitted with fever, backache, and pain in the lower limbs, with signs of radiculitis but no indication of inflammation or pain at the site of puncture. Magnetic resonance imaging revealed vertebral osteomyelitis at the fifth lumbar and first sacral vertebrae and an epidural abscess with compression of the nerve root. Treatment consisted of 2 g of ceftriaxone daily for 6 weeks, rest, and measures to assure local immobilization. Symptoms gradually improved and no surgical drainage measures were needed. The cause of osteomyelitis was never ascertained. Vertebral osteomyelitis is an unusual event after epidural anesthesia and there have been few opportunities to demonstrate a relationship. Such infections appear spontaneously in immunodepressed patients who undergo diagnostic procedures and treatments that lead to bacteremias with secondary colonization of spinal structures. The topography and characteristics of the infectious lesion, the patient's susceptibility, and the anesthetic procedure and pathogenic agent may help clarify the cause of the osteomyelitis.
