ABSTRACT
Four new HLA class I alleles, HLA-A*02:681, HLA-A*30:111, HLA-A*68:164 and HLA-B*35:01:46 were described in Spaniards.
Subject(s)
Alleles , HLA-A Antigens/genetics , HLA-A2 Antigen/genetics , HLA-B35 Antigen/genetics , HumansABSTRACT
The new B*50:51 allele was found in 3 Caucasians from Southern Spain.
Subject(s)
Alleles , HLA-B14 Antigen/genetics , Recombination, Genetic , Female , Humans , MaleABSTRACT
A novel HLA-A*30:99 allele was characterized in a Spanish individual.
ABSTRACT
Two new HLA-A null alleles were characterized, A*11:210N and A*26:107N.
Subject(s)
Genes, MHC Class I , HLA-A Antigens/genetics , Adult , Alleles , Amino Acid Sequence , Base Sequence , Humans , Molecular Sequence Data , Sequence Alignment , Sequence Homology, Amino Acid , Sequence Homology, Nucleic Acid , Spain , Tissue DonorsABSTRACT
HLA-B*44:203 shows one nucleotide difference to B*44:03:01 at codon 171 (TAC>CAC, Y171>H171).
Subject(s)
Alleles , Amino Acid Substitution , HLA-B Antigens/genetics , Polymorphism, Single Nucleotide , Base Sequence , Codon , Exons , Fetal Blood/chemistry , Fetal Blood/immunology , HLA-B Antigens/classification , HLA-B Antigens/immunology , Histocompatibility Testing , Humans , Molecular Sequence Data , Protein Isoforms/classification , Protein Isoforms/genetics , Protein Isoforms/immunology , Sequence Alignment , SpainABSTRACT
HLA-B*08:108 shows one nucleotide difference regarding B*08:01:01 at codon 109 (CTC>TTC, L109>F109).
Subject(s)
Alleles , Exons , HLA-B8 Antigen/genetics , Polymorphism, Single Nucleotide , Base Sequence , Bone Marrow Transplantation , Codon , HLA-B8 Antigen/immunology , Histocompatibility Testing , Humans , Molecular Sequence Data , Sequence Alignment , Sequence Analysis, DNA , Tissue Donors , White PeopleABSTRACT
Two novels alleles, HLA-A*01:128 and HLA-C*05:88 are characterized.
Subject(s)
Alleles , HLA-A Antigens/genetics , HLA-C Antigens/genetics , Amino Acid Sequence , Amino Acid Substitution , Bone Marrow Transplantation , Codon , HLA-A Antigens/immunology , HLA-C Antigens/immunology , Histocompatibility Testing , Humans , Molecular Sequence Data , Mutation , Sequence Alignment , Sequence Analysis, DNA , Spain , Tissue DonorsABSTRACT
Two novel HLA-B alleles were characterized. HLA-B*37:34 shows two nucleotide differences regarding B*37:10 at codons 79 (CGC>CGG) and 80 (ACC>ATC), resulting in one amino acid replacement at position 80 (T>I). HLA-B*44:152 differs from B*44:02:01 in one nucleotide at codon 81 (GCG>ACG) giving rise to a leucine to threonine substitution at position 81.
Subject(s)
HLA-B37 Antigen/genetics , HLA-B44 Antigen/genetics , Alleles , Amino Acid Sequence , Amino Acid Substitution , Genotyping Techniques , Histocompatibility Testing , Humans , Molecular Sequence Data , Point Mutation , Polymorphism, Single Nucleotide , Sequence Homology, Amino AcidABSTRACT
A*33:49 has one nucleotide change regarding A*33:01:01 at exon 3, producing an amino acid replacement at codon 97, M97 to I97.
Subject(s)
Alleles , Exons/genetics , HLA-A Antigens/genetics , Amino Acid Sequence , Amino Acid Substitution/genetics , Antigen Presentation/genetics , Cord Blood Stem Cell Transplantation , Histocompatibility Testing , Humans , Immunity/genetics , Molecular Sequence Data , Mutation/genetics , Polymorphism, Single Nucleotide , Sequence Analysis, DNA , SpainABSTRACT
DRB1*04:98 shows four nucleotide changes regarding DRB1*04:01:01, resulting in two amino acids replacement at positions A73G and A74R.
Subject(s)
Alleles , Amino Acid Substitution , HLA-DRB1 Chains/genetics , Mutation , DNA Mutational Analysis , Humans , SpainABSTRACT
A novel human leukocyte antigen-A allele, officially named A*3217, was found in two unrelated Spanish donors. A*3217 differs from A*320101 in a point mutation at codon 70 (CAC-->CAG), generating an amino acid substitution of His to Gln.
Subject(s)
Alleles , HLA-A Antigens/genetics , Amino Acid Sequence , Humans , Molecular Sequence Data , Spain , Tissue Donors , White People/geneticsABSTRACT
Two new human leukocyte antigen (HLA) class I alleles, A*3025 and Cw*0221, were identified in Spanish Caucasian donors by polymerase chain reaction-sequence-specific oligonucleotide and subsequently characterized by full coding region sequencing. HLA-A*3025 shows a single amino acid replacement at residue 70 (H>Q) compared with A*300201. HLA-Cw*0221 differs from Cw*020202 by a change at position 14 (H>Q).
Subject(s)
HLA-A Antigens/genetics , HLA-C Antigens/genetics , Alleles , Amino Acid Sequence , Base Sequence , Humans , Molecular Sequence Data , Sequence Alignment , White People/geneticsABSTRACT
Human leukocyte antigen (HLA) class I sequencing typing of a cord blood unit obtained from an Ecuadorian family showed the presence of the recently described A*6836 and B*3922 alleles. Sequences of these two antigens were confirmed and completed. Additionally, a new HLA-B allele, B*3579, was found in the same family, showing a difference of one amino acid residue at position 211 (Ala > Glu) compared with B*3543.
Subject(s)
Alleles , HLA-A Antigens/genetics , HLA-B35 Antigen/genetics , Sequence Analysis, DNA , Adult , Amino Acid Sequence , Base Sequence , Ecuador , Female , Humans , Infant, Newborn , Molecular Sequence DataABSTRACT
Three new HLA-A*02 alleles were completely characterized by sequencing-based typing (SBT). A*0281 and A*9224 showed eight clustered amino acid differences into the Bw4/Bw6 epitope at positions 74-83, regarding A*02010101. Both disclosed the same Bw4 motif described for A*25 and A*32 alleles. A*9224 has an additional mismatch at residue 265 (G>V) in the alpha3 domain, which has not been reported for any other human leukocyte antigen (HLA) class I molecule. HLA-A*0289 differed from A*02010101 in the conserved amino acid residue 192 (H>Q).
Subject(s)
HLA-A Antigens/genetics , White People/genetics , Amino Acid Sequence , Base Sequence , HLA-A2 Antigen , Haplotypes , Humans , Molecular Sequence Data , Sequence AlignmentABSTRACT
HLA-B*4077 differs from B*400101, B*4036, and B*401401 in five, four, and three nucleotides, respectively. However, amino acid alignment would indicate a closer relationship with B*4036 allele, showing a single amino acid replacement at codon 131 (R>S).
Subject(s)
Alleles , HLA-B Antigens/genetics , Adult , Base Sequence , Humans , Molecular Sequence Data , Point Mutation , Polymorphism, Single NucleotideABSTRACT
HLA-B*9523 differs from B*1591 and B*1503 in point nucleotide mutations at codon 167 (TCG --> TGG) and 152 (GAG --> GTG), respectively.
Subject(s)
HLA-B Antigens/genetics , Alleles , Base Sequence , DNA/genetics , Fetal Blood/immunology , Humans , Infant, Newborn , Molecular Sequence Data , Polymorphism, Genetic , Sequence Homology, Nucleic Acid , Spain , White People/geneticsABSTRACT
DRB1*1375 differs from DRB1*1304 allele in a point nucleotide mutation at codon 67 (ATC-->TTC), which produces an amino acid residue change from I to F.
