ABSTRACT
The chronic responses of aerobic granular sludge (AGS) to the presence of graphene oxide nanoparticles (GO NPs) (5, 15, 25, 35, 45, 55, 65, 75, 85, and 95â¯mg/L of GO NPs for 7 days) during biological wastewater treatment processes were investigated. Bioreactor performance, extracellular polymeric substance (EPS) secretion, and microbial community characteristics were assessed. The results showed that the effects of GO NPs on bioreactor performances were dependent on the dose applied and the duration for which it was applied. At concentrations of 55, 75, and 95â¯mg/L, GO NPs considerably inhibited the efficiency of organic matter and ammonia removal; however, nitrite and nitrate removal rates were unchanged. Biological phosphorus removal decreased even when only low concentrations of GO NPs were used. The secretion of EPS, which could alleviate the toxicity of GO NPs, also changed. The increased amount of nanoparticles also resulted in significant changes to the bacterial community structure. Based on the amplicon sequencing of 16S rRNA genes, Paracoccus sp., Klebsiella sp., and Acidovorax species were identified as the most tolerant strains.
