Epicutaneous sensitization with nematode antigens of fish parasites results in the production of specific IgG and IgE.

Fish consumption plays an important role in the human diet. Hoplias malabaricus, trahira, is a freshwater fish widely appreciated in several Brazilian states and it is frequently infected by Contracaecum multipapillatum third-instar larvae (L3). The aim of the present study was to evaluate the allergenic potential of the C. multipapillatum L3 crude extract (CECM). BALB/c mice were immunized intraperitoneally (ip) with 10 or 50 µg CECM associated with 2 mg of aluminium hydroxide on days 0, 14 and 48. The determination of specific IgG and IgE antibody levels was done after immunization, and the late immunity was evaluated by the intradermal reaction in the ear pavilion. Epicutaneous sensitization was performed in the dorsal region, with antigenic exposure via a Finn-type chamber, containing 100 µg of chicken ovum albumin (OVA) or 100 µg CECM. After the exposures, the specific antibody levels were determined. In the ip immunization, there was a gradual increase in IgG antibody levels, independent of CECM concentration. In relation to IgE production, it was transitory, and immunization with 10 µg was more efficient than that of 50 µg. The same result was observed in the cellular hypersensitivity reaction. In the case of antigen exposure by the epicutaneous route, it was verified that only CECM was able to induce detectable levels of specific IgG and IgE antibodies. In the present study it was demonstrated that both intraperitoneal immunization and epicutaneous contact with C. multipapillatum larval antigens are potentially capable of inducing allergic sensitization in mice.

Philometra saltatrix (Nematoda: Philometridae) in the ovary of the bluefish, Pomatomus saltatrix (Linnaeus, 1766), off the coast of the state of Rio de Janeiro, Brazil.

The aims of the present study were to identify and describe the occurrence of nematode parasites in the gonads of bluefish Pomatomus saltatrix from off the coast of the state of Rio de Janeiro, Brazil. Only females were found to be parasitized by the nematodes, which were identified as P. saltatrix using morphological, morphometric and molecular data. Infection of female bluefish by this nematode had the following values: prevalence, 48.7%; mean intensity, 2.6; mean abundance, 1.3; and range of infection, 1-10 specimens. Histopathological examination of transverse and longitudinal sections of the parasitized ovaries showed nematodes at different stages of development among oocytes, but no indication of any associated inflammatory reaction. The presence of nematodes in the ovaries of bluefish is an important indication of fish hygiene, and parasitized fish are usually rejected by consumers because of their repugnant appearance.

Parasitism by Monogenoidea in Piaractus mesopotamicus (Characiformes, Characidae) cultivated in Paraná River (Brazil) / Parasitismo por Monogenoidea em Piaractus mesopotamicus (Characiformes, Characidae) cultivados no rio Paraná (Brasil)

Abstract This study investigated the occurrence, prevalence, mean abundance and mean intensity of monogenoidean parasites in Piaractus mesopotamicus farmed in cages in the reservoir of the Itaipu Hydroelectric Power Station, Paraná River, Brazil. The parasite distribution pattern and the correlation of prevalence and abundance with the total length of hosts were also investigated. Four monogenoidean species were collected: Anacanthorus penilabiatus, A. toledoensis, Mymarothecium ianwhitingtoni and M. viatorum. All the parasites collected in P. mesopotamicus showed the typical aggregated distribution pattern, and the abundance and the prevalence did not shown any correlation with the total length of hosts.

Resumo Neste estudo foi investigada a ocorrência, prevalência, abundância média e intensidade média de Monogenoidea parasitos de Piaractus mesopotamicus, cultivados em tanques-rede no reservatório da Usina Hidrelétrica de Itaipu, rio Paraná, Brasil. Foram investigados ainda o padrão de distribuição parasitária e a correlação entre a prevalência e abundância com o comprimento total dos hospedeiros. Quatro espécies de Monogenoidea foram coletadas: Anacanthorus penilabiatus, A. toledoensis, Mymarothecium ianwhitingtoni e M. viatorum. Todos os parasitos coletados em P. mesopotamicus apresentaram típico padrão de distribuição agregada e não foi observada correlação entre a abundância e a prevalência e o comprimento total dos hospedeiros.

Parasitism by Monogenoidea in Piaractus mesopotamicus (Characiformes, Characidae) cultivated in Paraná River (Brazil).

This study investigated the occurrence, prevalence, mean abundance and mean intensity of monogenoidean parasites in Piaractus mesopotamicus farmed in cages in the reservoir of the Itaipu Hydroelectric Power Station, Paraná River, Brazil. The parasite distribution pattern and the correlation of prevalence and abundance with the total length of hosts were also investigated. Four monogenoidean species were collected: Anacanthorus penilabiatus, A. toledoensis, Mymarothecium ianwhitingtoni and M. viatorum. All the parasites collected in P. mesopotamicus showed the typical aggregated distribution pattern, and the abundance and the prevalence did not shown any correlation with the total length of hosts.

Resposta imunológica a antígenos de Hysterothylacium deardorffoverstreetorum de peixes teleósteos / Imunne response against Hysterothylacium deardorffoverestreetorum from teleost fish

Anisaquidose é uma doença provocada por parasitos da família Anisakidae e se caracteriza por manifestações gastrointestinais e alérgicas. O Anisakis simplex é o parasito mais patogênico ao homem e altamente alergênico. Porém, outros anisaquídeos também são danosos aos humanos, mas é desconhecida a imunogenicidade dessas larvas. O objetivo deste trabalho foi avaliar o potencial imunogênico do parasito Hysterothylacium deardorffoverestreetorum (HD) em modelo murino. Camundongos da linhagem BALB/c foram divididos em três grupos experimentais e receberam as preparações antigênicas obtidas de larvas de HD: extrato bruto de larvas (EBH), extrato secretado/ excretado de larvas (ESH) e extrato bruto de larvas após excreção/secreção (EEH). Amostras séricas foram obtidas em diferentes dias após imunização para determinação dos níveis de anticorpos específicos pelo ensaio imunoenzimático (ELISA). Os resultados demonstram aumento na produção de imunoglobulina (Ig) G após a segunda imunização, com aumento progressivo após a terceira imunização. Já em relação à IgE, a reatividade foi mais tardia, demonstrando aumento progressivo após a terceira imunização. Foi avaliada a imunidade celular por meio da intradermorreação, como resultado estatisticamente significativo em relação ao controle utilizado. Este experimento é a primeira descrição da potencialidade patogênica desse parasito em mamíferos e representa um avanço no diagnóstico da anisaquidose humana.(AU)

Anisaquidosis is a disease caused by parasites of Anisakidae family and is characterized by gastrointestinal and allergic reactions. The Anisakis simplex is a more pathogenic Anisakidae to humans and is highly allergenic. However, other species of this family also have characteristics that are harmful to humans, but little is known about the immunogenicity this parasites. The objective of this study was to experimentally assess the immunogenic potential of the parasite Hysterothylacium deardorffoverestreetorum (H.D) in mice. Mice of inbred BALB/c strain were divided into three groups and received three immunizations of the following antigenic preparations obtained from L3 larvae H.D: Crude larval extract of H.D (CEH) Extract secreted / excreted larvae H.D. (ESH) and crude extract of larvae after excretion / secretion (EEH). Serum samples were obtained on different days after immunization to determine the levels of circulating specific antibodies by enzyme-linked immunosorbent assay (ELISA). The results show increased production of immunoglobulin (Ig) G after the second immunization with a gradual increase after the third immunization. Regarding IgE reactivity, this occurred later, demonstrating a progressive increase only after the third immunization. Cellular immunity was evaluated by intradermal, and showed statistically significant result compared to the control used. This experiment is the first description of the pathogenic potential of this parasite in mammals and represents a breakthrough in the diagnosis of human Anisakidosis.(AU)

Primeiro relato de Hysterothylacium deardorffoverstreetorum (Raphidascarididae) em bijupirá de criação, Rachycentron canadum (Linnaeus 1766), no Brasil / First report of Hysterothylacium deardorffoverstreetorum (Raphidascarididae) in cobia (Rachycentron canadum (Linnaeus 1766) farming in Brazil

O objetivo do presente estudo foi registrar a ocorrência de larva de nematoide da família Raphidascarididae, Hysterothylacium deardorffoverstreetorum, em bijupirá Rachycentron canadum (Linnaeus, 1766), criado e alimentado com ração e "trashfish" em fazendas marinhas localizadas no Rio de Janeiro, Brasil, alertando para possíveis riscos zoonóticos ao se usar esse tipo de alimentação para criação de peixes. Foram necropsiados 15 animais. Os parasitas encontrados foram coletados, fixados e, posteriormente, conservados em álcool a 70°GL, clarificados e identificados. Em todos os animais necropsiados, apenas um espécime estava parasitado com uma larva de Hysterothylacium deardorffoverstreetorum na serosa do fígado do peixe e conclui-se que a presença desse parasita em bijupirá de criação alimentado por "trashfish pode estar associada à alimentação, indicando, assim, um potencial risco zoonótico.(AU)

The aim of this study was to record the occurrence of nematode larvae of the Raphidascarididae family, Hysterothylacium deardorffoverstreetorum in cobia Rachycentron canadum (Linnaeus, 1766) grown and fed with trashfish in marine farms located in Rio de Janeiro, Brazil, warning regarding possible zoonotic risk using this type of food for fish. Fifteen animals were necropsied. Parasites found were collected, fixed and later preserved in alcohol 70°GL, clarified and identified. In all animals necropsied, only one specimen was infested with a Hysterothylacium deardorffoverstreetorum larvae in the serosa of fish liver. It is concluded that the presence of the parasite in cobia culture fed with trashfish may be associated with a potential zoonotic risk.(AU)

Spontaneous multi-cystic peripheral ameloblastoma in the freshwater angelfish, from the Brazilian state of Pará / Ameloblastoma periférico multicístico espontâneo em Acará Bandeira, no Estado do Pará, Brasil

Este trabalho registra a ocorrência espontânea de ameloblastoma em P. scalare. O tumor foi obtido a partir de um exemplar de Acará Bandeira, sendo fixado, seccionado, e os fragmentos processados para microscopia de luz e microscopia eletrônica de varredura (MEV). O exame macroscópico evidenciou uma massa tumoral que se estendia do rebordo alveolar do maxilar superior à face externa labial. À MEV, a neoplasia apresentou uma série de espículas. Microscopicamente, percebia-se um processo neoplásico constituído por tecido conjuntivo de característica mixoide, ricamente vascularizado, onde eram observados fragmentos de tecido osteoide. A amostra revelou proliferação neoplásica do epitélio odontogênico, onde as células neoplásicas se organizavam na forma de paliçada. Alterações histopatológicas em peixes têm sido úteis biomarcadores do efeito à exposição a substâncias tóxicas, sendo as neoplasias lesões específicas, comumente encontradas em peixes de áreas poluídas, revelando uma associação entre as lesões e a exposição a irritantes.(AU)

Total Mercury in Carnivorous Fish from Brazilian Southeast.

Total mercury concentrations were determined by atomic absorption spectrometry in muscle tissue of two commercially important species of carnivorous fishes croaker (Micropogonias furnieri) and cutlassfish (Trichiurus lepturus) caught in Itaipu estuary, Rio de Janeiro. In this study, whitemouth croaker presented more mercury than Atlantic cutlassfish. Bioaccumulation differences can be explained by the biological behavior of juveniles whitemouth croaker, that remain in the estuary up to maturity, which makes them good indicators of local environmental impacts (0.110 mg Kg(-1) HgT). It also can be explained by differences in nutritional requirements between the different life stages of two species. The analysis showed the presence of low levels of the metal. However, our results suggest a possible risk to human health, depending on the level of fish consumption.

Immunogenic activity of the fish tapeworm Pterobothrium heteracanthum (Trypanorhyncha: Pterobothriidae) in BALB/c mice.

The aim of this study was to verify the immunogenicity of Pterobothrium heteracanthum (Cestoda: Trypanorhyncha) crude protein extract (PH-CPE) in BALB/c mice. The parasites were obtained from Micropogonias furnieri (Osteichthyes: Sciaenidae). Groups of six mice were each immunized with 10, 50 or 100 µg of PH-CPE, on days 0 and 35. Both specific IgG and IgE responses were developed after immunization. The immunoblot assay revealed that specific IgG recognizes PH-CPE proteins with two molecular weight ranges, 60-75 and 30-40 kDa, and that IgE recognizes larger proteins over 120 kDa. This appears to be the first report on the immunogenicity of metacestodes within the Pterobothriidae and that PH-CPE is a potential inducer of a specific IgE response.

Distribution of Po-210 in two species of predatory marine fish from the Brazilian coast.

Polonium-210 ((210)Po) concentration was quantified in the muscle tissue and organs of two predatory marine fishes (Genypterus brasiliensis and Cynoscion microlepidotus) from Cabo Frio, Rio de Janeiro, Brazil. The species C. microlepidotus, a benthic carnivore, registered higher (210)Po in its tissue. The organs associated with digestion displayed the maximum radionuclide compared with other organs. The average activity was 2 mBq kg(-1) for G. brasiliensis and it was 6 mBq kg(-1) for C. microlepidotus. The activity concentrations varied significantly between the species and among organs.

An anatomopathological study of hepatic coccidiosis (Calyptospora sp. ) in the Acará-pixuna, Aequidens plagiozonatus Kullander, 1984 from the Brazilian state of Pará / Estudo anatomopatológico de coccidiose hepática (Calyptospora sp. ) em Acará-pixuna, Aequidens plagiozonatus Kullander, 1984, no Brasil, estado do Pará

The present study focuses on the anatomopathological alterations provoked by parasitism by Calyptospora sp. in 40 specimens of Aequidens plagiozonatus collected in Pará, Brazil. Examinations of the fresh material by compression of the hepatopancreas and histological sections showed immature forms and oocysts characteristic of the genus Calyptospora, in addition to a large quantity of melanomacrophagic centers spread throughout the organ. No significant inflammation of the hepatic tissue was observed. The melanomacrophagic centers and the compression of the hepatocytes are part of the response of the host to the parasite. This study represents the first record of parasitism by Calyptospora in A. plagiozonatus.

Descreveram-se as alterações anatomopatológicas provocadas pelo parasitismo por Calyptospora sp. em 40 espécimes de Aequidens plagiozonatus, provenientes do município de Peixe-boi, Pará, Brasil. Foram encontradas formas imaturas e oocistos característicos do gênero Calyptospora, nos exames frescos por compressão e cortes histológicos, além de um grande número de centros melanomacrofágicos dispersos por todo o órgão. Digna de nota foi a ausência de inflamação significativa no tecido hepático. Centros melanomacrofágicos e compressão dos hepatócitos estão envolvidos na resposta do hospedeiro ao parasito. Este é o primeiro registro de ocorrência de parasitismo por Calyptospora sp. na espécie estudada.

Development and bacteriological, chemical and sensory characterization of fishburgers made of Tilapia minced meat and surimi / Desenvolvimento e caracterização bacteriológica, química e sensorial de "fishburgers" de polpa e "surimi" de tilápia

In this study fishburger samples obtained from tilapia minced meat and surimi retrieved from fillet frames - filleting process waste - have been assessed. Four different preparations of fishburger were made. Chemical analyses was performed for protein, lipids, moisture and ash assessments, as well as bacteriological analyses through mesophilic aerobic heterotrophic and psychrotrophic bacteria counts, isolation and identification of Salmonella spp., coagulase-positive Staphylococcus and fecal coliforms. Instrumental measurements were also carried out for texture and tested sensory acceptance. The costs of the ingredients used in the preparation of formulas were also assessed. Microbiological standards, established by current regulations, were met by all samples. No significant gap (P>0.05) was detected among the percentage of protein, moisture and ashes, while a significant gap (P<0.05) was verified for lipids. A significant gap (P<0.05) for the instrumental measurements of texture was also observed. Both surimi and minced meat fishburger presented good acceptance on sensory tests for taste, texture and overall impression. The use of smoked fish flavoring and surimi caused a considerable increase in costs due to ingredient acquisition. This study shows a positive result for the industrialization and consumption potential of fishburgers made of minced fish retrieved from fillet frames.

Foram avaliadas amostras de "fishburgers" elaboradas com polpa e "surimi" de tilápia obtidos a partir do espinhaço residual da linha de filetagem. Realizaram-se análises químicas de proteína, lipídios, umidade e cinzas; análises bacteriológicas de bactérias heterotróficas aeróbias mesófilas e psicrotróficas; isolamento e identificação de Salmonella spp., Staphylococcus coagulase positiva e coliformes fecais; análises instrumentais de textura; teste de aceitação sensorial e levantamento de custo dos ingredientes utilizados na elaboração das formulações. Todas as amostras analisadas atenderam aos padrões microbiológicos estabelecidos pela legislação vigente. Não ocorreu diferença significativa (P>0,05) entre os percentuais de proteína, umidade e cinzas, tendo sido observada diferença em relação ao percentual de lipídios entre as amostras. Observou-se diferença significativa entre as amostras avaliadas por análise instrumental, em relação à textura (P<0,05). Tanto o "fishburger" elaborado com "surimi" quanto o elaborado com polpa, foram aceitos sensorialmente em relação ao sabor, textura e impressão global. A utilização de flavorizante à base de peixe defumado e do "surimi", resultou em aumento considerável no custo com aquisição de ingredientes. Observa-se um resultado positivo em relação ao potencial de industrialização e consumo do "fishburger" elaborado com polpa de tilápia obtida do espinhaço residual da filetagem.

Anisakidae nematodes and Trypanorhyncha cestodes of hygienic importance infecting the king mackerel Scomberomorus cavalla (Osteichthyes: Scombridae) in Brazil.

From February to October 2007, thirty specimens of the king mackerel, Scomberomorus cavalla (Cuvier, 1829) were purchased from markets in the municipalities of Niterói and Rio de Janeiro. The fishes were measured, filleted and further had their organs investigated for helminths. Ten out of the thirty fish specimens were parasitized with anisakid nematodes represented by Anisakis sp. and Contracaecum sp. with prevalence of 1% and 16%, mean intensity of 2 and 3.31 and mean abundance of 0.02 and 0.53, respectively. The infection range with Contracaecum sp. was 1-9. The sites of infection were the stomach serosa and mesentery. Seventeen fish specimens (53%) out of the 30 investigated were parasitized with Trypanorhyncha metacestodes, identified as Callitetrarhynchus gracilis, Pterobothrium crassicole, Callitetrarhynchus speciosus and Tentacularia coryphaenae in the mesentery, with prevalence of 26, 20, 6, 3%, intensity and mean intensity of 3.25, 3.5, 1, 2 and mean abundance of 0.86, 0.7, 0.06 and 0.06, respectively. The infection range due to C. gracilis and P. crassicole were of 1-5 and 1-20, respectively. Anisakis sp., C. speciosus and P. crassicole are reported in S. cavalla for the first time. Considerations on the zoonotic potential of the parasites and their rules in sanitary inspection are presented.

Distribuição do metal mercúrio em atum (Thunnus albacares) e pescada bicuda (Cynoscion microlepidotus) capturados no litoral do Rio de Janeiro, Brasil / Distribution of metal mercury in tuna (Thunnus albacares) and weaker fish (Cynoscion microlepidotus) captured in Rio de Janeiro coast, Brazil

O presente estudo verificou a cinética mercurial em órgãos, tecido muscular e conteúdo estomacal de duas espécies de peixes marinhos predadores, identificando os locais de maior acumulação desse metal. Para o processo analítico, foi utilizada a técnica de espectrofotometria de absorção atômica por arraste de vapor a frio. A distribuição de Hg nas porções analisadas apresenta ampla dispersão. Para o atum, a variação foi de 0,004 µg.g-1 no fígado, nas brânquias e baço, e 0,172µg.g-1 no tecido muscular. Para a pescada bicuda, a variação foi entre 0,013µg.g-1 nas brânquias a 0,250µg.g-1 no tecido muscular. Este último demonstrou ser, nas duas espécies, a porção de maior concentração do metal, atum = 0,080±0,050µg.g-1 e pescada bicuda = 0,187±0,030µg.g-1. Os locais de menor concentração na pescada foram as brânquias 0,029±0,031µg.g-1, e no atum os olhos (0,013±0,008µg.g-1).

The mercuric kinetic in organs, muscular tissue, and stomach contents of two species of marine fish predators was evaluated by the identification of the places showing higher accumulation of the metal. For the analytical process, the used technique was cold vapor atomic absorption spectrometry. The results demonstrated that the distribution of Hg in the analyzed portions presented wiede dispersion. For Thunnus albacares, the variation was of 0.004µg.g-1 in the liver, gills and spleen, and 0.172µg.g-1 in the muscular tissue. For Cynoscion microlepidotus, the variation was between 0.013µg.g-1 in gills and 0.250µg.g-1 in muscular tissue. The latter was the portion of higher concentration of this metal in both species, T. albacares = 0.080±0.050µg.g-1 and C. microlepidotus = 0.187±0.030µg.g-1, while less concentrations were found in the gills (0.029±0.031µg.g-1) in C. microlepidotus and in the eyes (0.013±0.008µg.g-1) in T. albacares.

Total mercury in sharks along the southern Brazilian Coast / Mercúrio total em tubarões capturados na costa brasileira

Pesquisou-se a concentração de mercúrio total na porção muscular de 39 exemplares de tubarões de três diferentes espécies Prionace glauca, Isurus oxyrhynchus, Sphyrna zygaena, capturados na costa sul do Brasil, Santa Catarina. O teor de mercúrio foi determinado por espectrofotometria de absorção atômica por vapor frio. Observou-se ampla variação na concentração de mercúrio total com valor individual máximo excedendo o limite estabelecido pela legislação brasileira, de 1.0µg.g-1 em um exemplar fêmea de P. glauca. Nessa espécie, a concentração de Hg-total aumentou proporcionalmente ao comprimento total (r=0,62; P<0,0001). Não foi evidenciada diferença estatisticamente significativa entre exemplares fêmeas e machos

The taxonomy of some Poecilacanthoidea (Eucestoda: Trypanorhyncha) from elasmobranchs off the southern coast of Brazil.

Specimens of elasmobranchs, collected in the State of Santa Catarina, Brazil, were parasitized with Poecilacanthoidea trypanorhynch cestodes. Sharks of the species Prionace glauca harboured Callitetrarhynchus gracilis and Floriceps saccatus, and those of the species Sphyrna zygaena were infected with Callitetrarhynchus speciosus. Details of the proglottids of F. saccatus, provided by bright-field, and/or scanning electron microscopy, are described. Adults of F. saccatus are reported for the first time in the Brazilian coast.

Taxonomic reports of Homeacanthoidea (Eucestoda: Trypanorhyncha) in Lamnid and Sphyrnid elasmobranchs collected off the coast of Santa Catarina, Brazil.

Elasmobranch specimens of lamnid and sphyrnid captured in 1999 in the State of Santa Catarina, Brazil, were parasitized with homeacanthoid trypanorhynch cestodes: Isurus oxyrinchus Rafinesque, 1810 with Nybelinia lingualis (Cuvier, 1817) Dollfus, 1929; Sphyrna zygaena (Linnaeus, 1758) with Heteronybelinia rougetcampanae (Dollfus, 1960) Palm, 1999. New details of internal morphology and/or scolex and/or proglottid surface ultrastructure are given. Adults of N. lingualis are reported for the first time in the Brazilian coast.

Nematodes of elasmobranch fishes from the southern coast of Brazil.

New records for nematode species recovered from elasmobranch fishes in Brazil are established and new systematical arrangements proposed. Parascarophis sphyrnae Campana-Rouget, 1955 from the spiral valve of Sphyrna zygaena is referred for the first time in South America as a new host record. Procamallanus (S.) pereirai Annereaux, 1946, from the spiral valve of Raja castelnaui is reported parasitizing an elasmobranch host. Nematode larvae of the genera Anisakis, Contracaecum, Pseudoterranova and Raphidascaris are listed from the stomach and spiral valves of several hosts. Anisakidae larvae previously referred in Brazil in the genus Phocanema should be reallocated in Pseudoterranova. Nematodes of the genera Anisakis, Contracaecum, Pseudoterranova and Raphidascaris are reported for the first time parasitizing elasmobranchs in Brazil.

Digenea and acanthocephala of elasmobranch fishes from the southern coast of Brazil.

New records for helminth species recovered from elasmobranch fishes in Brazil are established. Digenean and acanthocephalan parasites of elasmobranch fishes are reported from the southern coast of Brazil: Otodistomum veliporum (Creplin, 1837) Stafford, 1904 (Digenea: Azygiidae) in the stomach and spiral valve of Dipturus trachydermus and in the spiral valve of Squatina sp. Cystacanths and juveniles of the acanthocephalans Corynosoma australe Johnston, 1937 and Corynosoma sp., in the spiral valve of Squatina sp., Galeorhinus galeus and Hexanchus griseus and in the stomach of Squalus megalops; a juvenile of Gorgorhynchus sp., in the spiral valve of Sphyrna zygaena. Dipturus trachydermus and Squatina sp. are new host records for O. veliporum. Digeneans and acanthocephalans are reported for the first time parasitizing elasmobranch fishes in Brazil.