[Sexual assault in the woman]. / Sexuální násilí na zenách.

OBJECTIVE: Retrospective analysis of the population of women examined for suspicion of sexual assault to compare the situation in the Czech Republic with the world's published works. DESIGN: Retrospective analysis. SETTING: Department of Obstetrics and Gynecology, 1st Medical Faculty of Charles University and the Faculty Hospital Na Bulovce Prague. METHODS: Retrospective analysis of medical reports for all women examined for suspicion of sexual violence during the period from January 2007 to December 2008. RESULTS: During observed period 37 women were examined, average age of examined women was 26.2 years, range 15-51 years, 76% of females younger than 29 years, 16% of examined women were from abroad. We have found no differences in occurrence throughout the year. In 3 cases there were more aggressors, in 30% of cases the alleged perpetrator was a known person or relative. In 54% of cases completed vaginal intercourse with ejaculation allegedly took place. We detected one case of forced oral sex and one case of forced anal sex. In 19% touching was involved. 22% of women did not know whether coitus really occurred--in 14% the alleged victim was under the influence of alcohol, in 3 cases under the influence of drugs, including one case of drinking an unknown substance in a beverage. Obvious signs of violence were found in 27% of cases, of which mostly in the face (14%), the extremities (8%) and only in two cases on the genitals (5%). No woman did require medical treatment of injuries. CONCLUSION: Our experience is similar to the published world data. In our group there were more strangers among the perpetrators. We did not record any use of a weapon in the enforcement of sexual contact. It is necessary to examine the alleged victims whole body thoroughly, signs of violence were more frequent in extragenital localization.

[The science-meter indicators (citation index, impact factor, Hirsch-index) and the evaluation of the scientific activity in medicine]. / Scientometrické ukazatele (citacní index, impakt faktor, Hirschuv index) a hodnocení vedecké práce v medicíne.

OBJECTIVE: An overview and characteristics of the main science-meter indicators (citation index, impact factor, Hirsch-index) and its role and importance in the evaluation of the scientific activity in medicine and other scientific disciplines. TYPE OF STUDY: A review. SETTING: Department of Obstetrics and Gynecology, 1st Medical Faculty, Charles University and General Faculty Hospital in Prague. METHODS: An analysis of the publications (including citation databases and information retrieval) related to the impact factor of the selected journals and H-index of the employees of the significant ob/gyn institutions in the Czech Republic. CONCLUSIONS: Although the science-meter indicators generate only the approximate values, they are the necessary part of the evaluation system of the scientific activity. To be objective in the evaluation in the scientific activity is recommended to combine the science-meter indicators with the other methods, e.g. an individual evaluation of the results by "peer review" method.

Biochemical characterization of broad-specificity enzymes using multivariate experimental design and a colorimetric microplate assay: characterization of the haloalkane dehalogenase mutants.

The pH indicator dye-based colorimetric method and multivariate experimental design were used for the systematic biochemical characterization of the broad-specificity enzymes haloalkane dehalogenases. Halogenated compounds for characterization of the enzymes were selected using Principal Component Analysis. The substrates were characterised by 24 physico-chemical and structural descriptors. Thirty-four substrates were selected for testing out of 194 halogenated compounds. Relative activities determined using the optimised colorimetric microplate assay were validated against the catalytic constants determined by gas chromatography. The applicability of the assay was tested with F151L, F154L and F169L mutants of the haloalkane dehalogenase from Sphingomonas paucimobilis UT26.

[Activity of the 2nd Clinic of Gynecology and Obstetrics from its founding to the end of World War II (1920-1945)]. / Cinnost II. Gynekologicko-porodnické kliniky od jejího vzniku do konce druhé svetové války (1920-1945).

History of the IInd Gynaecological and Obstetric Clinic of 1st Faculty of Medicine, Charles University in Prague is recorded here from its establishment in 1920 to the end of World War II. The activities of the Clinic are depicted in characterization of prominent personalities who worked here in this period. The characterization is focussed mostly on head physicians and their clinical, pedagogical and scientific activities and their works published.

Regional and cellular differences in fucosylation of glycomacromolecules in the mouse brain. A biochemical and autoradiographic study of early postnatal and adolescent animals.

Mice of CBA strain of both sex were injected with [3H]fucose at age 2, 6, 12 or 30 days and the incorporation was determined biochemically 45, 90 and 180 min later. Biochemical measurements of the whole brain (at 90 min postinjection interval) revealed a stepwise age decrease in the amount of incorporated isotope (per mg protein). The amount of [3H]fucose available in the brain has however been found to decrease with age while the degree of its utilization increases. Thus, after correction of the data on [3H]fucose incorporation for the pool of the free isotope, a transient increase in fucosylation rate appeared at postnatal day 12 while the differences among 2-, 6- and 30-day-old animals became negligible. Further, the synthesis of fucosylated soluble glycomacromolecules appeared relatively higher at postnatal day 2 than in older age groups. Examination of different brain regions revealed that the rate of fucosylation is relatively highest in the olfactory bulbs; this prevalence starts appearing with age and becomes most evident in 30-day-old mice. Autoradiography carried out with 6- and 12-day-old animals revealed that the incorporation of [3H]fucose into meningovascular apparatus and the choroid plexus is a few times higher than into brain parenchyma. The regional differences appeared to be similar to those indicated by the biochemical data. Cellular analysis has shown that the incorporation is relatively higher in the cytoplasm of large projecting neurons of the cerebellum, hippocampus and the olfactory bulbs; in the latter region high amounts of macromolecule-bound [3H]fucose also appeared in the synaptic glomeruli.(ABSTRACT TRUNCATED AT 250 WORDS)

DNA synthesis in the cerebellar Purkinje neurons of early postnatal rats: an extra DNA or a repair process?

DNA synthesis and Feulgen - DNA content in Purkinje neurons were examined in early postnatal rats exposed to cis-dichlorodiammineplatinum II (cis-DDP) and (3H) thymidine. Autoradiographic data revealed a significant although low labelling in control rats on postnatal day 10 to 11; the incorporation deeply decreased after cis-DDP administration. Cytophotometry showed formation of some Purkinje cells with hyperdiploid DNA content in control animals between postnatal day 11 and 16, whereas in cis-DDP treated rats all cells remained diploid. A close correlation between the formation of hyperdiploid Feulgen-DNA nuclei and the incorporation of (3H) thymidine provides new, indirect support for an actual DNA surplus in some Purkinje cells, although the incorporation of some of (3H) thymidine due to an enhanced turnover and or repair of DNA in Purkinje cells of 10 to 11 days old rats is not excluded.