Preservation fluids of heritage anatomical specimens - a challenge for modern science. Studies of the origin, composition and microbiological contamination of old museum collections.

Anatomical museums preserve specimens of great historical value and undiscovered scientific potential. However, frequently these collections lack documentation of the techniques of preparation and the composition of preservative substances (conservation principles). This poses a huge problem for the care and preservation of these materials, more so because understanding this issue requires knowledge of fundamentals from different scientific disciplines. The aim of the research was to obtain information about the composition of substances used to preserve historic specimens, as well as to conduct a microbiological assessment of the specimens to detect possible factors causing their deterioration. Furthermore, we wanted to fill an existing gap in the literature, as there is a lack of reports on analytical methods that could be successfully applied by anatomists involved in the daily care of museum collections in human anatomy departments. The starting point was the analysis of the sources and history of the collections, on which basis the choice of research methods was made. Methods based on simple chemical reactions and specialised methods (such as gas chromatography-tandem mass spectrometry, Fourier transform infrared spectroscopy, inductively coupled plasma optical emission spectroscopy) were used in the analyses of the composition of fluids. Microbiological analyses were based on culture and isolation methods, analysis of microscopy slides and matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry analysis. As a result of these analyses, some components of the preservative mixtures and their concentrations were determined. The presence of methanol, ethanol, formaldehyde and glycerol was detected, among other chemicals. The concentrations of these substances were different between the samples and their determination required the use of a variety of methods suitable for the individual components of the preservative mixture. In microbiological tests, both bacteria and fungi were isolated from swabs taken from anatomical specimens. The bacterial flora was less numerous than the fungal flora. Among the bacteria, environmental Gram-positive Bacillus cereus, Bacillus thuringiensis and a rare bacterium of the Cupriavidus genus were isolated, whereas among the fungal organisms, the yeast-like fungi Candida boidinii and Geotrichum silvicola as well as mould fungi Penicillium sp. and Fusarium sp. were detected. However, the microscopic evaluation showed a greater diversity of microorganisms, which may be related to the fact that many environmental bacteria cannot be cultured using classical methods, but can be observed under the microscope. The results of the research made it possible to draw conclusions about the mutual influence of physical, chemical, and microbiological factors on the condition of historical anatomical specimens. In the course of the research, information was obtained on the processes which could have taken place during the storage of these collections. Maintaining the integrity of a container housing a preserved anatomical specimen has a major impact on maintaining the concentration of preservative fluid and keeping the specimen environment sterile. Many conservation procedures for historical specimens carried out nowadays pose a risk of destroying valuable specimens, as well as a health risk for the person carrying out the work. The exploration of the topic of conservation of anatomical specimens, especially those that lack documentation of their origin, is a key issue in current research on historical collections of anatomical specimens.

Terra Incognita in anatomical museology - A literature review from the perspective of evidence-based care.

BACKGROUND: The care of historical collections in anatomical museums is a highly specialized subject requiring advanced knowledge. When searching for practical information on this subject, the authors were not able to find appropriate literature based on scientific research. The absence of this literature is probably due to the specialized nature of the subject and the poorly defined classification of this type of museum. The purpose of this study is to conduct a systematic literature review to identify (i) the current state of knowledge of anatomical museology and (ii) the nature and determinants of ongoing research on anatomical museum objects. MATERIALS AND METHODS: A systematic search of the main electronic databases (PubMed, Google Scholar, Scopus) was conducted to identify relevant studies. The records retrieved were categorized according to thematic similarity and scientific content. Based on these groupings, statistics were created based on the number of eligible papers in each particular group. RESULTS: 61 % of the papers retrieved addressed the history, social issues, or related aspects of contemporary museology; 8 % were technical papers; and 31 % research papers, of which only 8 % were focused on the museum object as the topic of the research. The paper retrieved showed a predominance of works in cultural studies, with surprisingly few applicable to museum practice. Furthermore, there was a lack of consensus in the literature regarding the classification of anatomical museums. CONCLUSIONS: Anatomical museology is a poorly defined concept in the scientific literature and it is a rare topic in contemporary work by anatomical practitioners. The literature review revealed that the debate about the fate of anatomical museums encompasses a broad spectrum of diverse, often disparate scientific fields as well as economic factors that influence the present status and future of these institutions. For these reasons, museum object research is problematic in design, may not be considered worthwhile, or is unattractive from an institutional perspective. The literature survey showed that there is a paucity of work in the available modern literature that provides significant support for museum anatomists.

Subsistence huniing for turtles in northwestern Ecuaoor / Aprovechamiento de subsistencia de la fauna de tortugas en el noroccidente de Ecuador

We describe the subsistence exploitation of an entire turtle fauna in Esmeraldas Province, Ecuador. We collected first-hand accounts and witnessed a number of capture techniques used by rural Afroecuadorian and Chachi inhabitants of the Cayapas-Santiago river basin. The diversity of techniques indicated a practical knowledge of the ecology of the species. Chelydra acutirostris, Kinosternon leucostomum, Rhinoclemmys annulata, melanosterna, and R. nasuta were captured and eaten. Poziando involved cleaning pools in a stream bed during the relatively dry season by removing live plants, organic detritus, and then seining with baskets; we observed R. melanosterna and K. leucostomum captured in this way. Pitfall traps baited with fruit were used to catch R. melanosterna during forays on land. Basket traps ("canasto tortuguero") with a wooden slat funnel across the opening are floated with balsa lashed to the sides. Banana or Xanthosoma leaf bait in the basket traps caught R. melanosterna, R. nasuta, and K. leucostomum. Marshy areas were probed for R. melanosterna and K. leucostomum. Direct capture by hand was also common. Turtles were relished as food items; all turtles captured were consumed, usually in soup or stew. Use of turtles for food in the region was pervasive, perhaps because fish and game populations were depleted.

Describimos la cacería de subsistencia de la fauna de tortugas en la provincia de Esmeraldas, Ecuador. Hemos recogido testimonios de primera mano y fuimos testigos de una serie de técnicas de captura utilizadas por los habitantes rurales afroecuatorianos y chachis de la cuenca de los ríos Cayapas-Santiago. La diversidad de técnicas indica un conocimiento práctico de la ecología de las especies. Chelydra acutirostris, Kinosternon leucostomum, Rhinoclemmys annulata, R. melanosterna y R. nasuta fueron capturadas y utilizadas como alimento. El método de "pozeo" consiste en limpiar las pozas o quebradas durante la estación de menos lluvia; en el proceso se remueven las plantas vivas y detritus orgánicos y las tortugas se cazan con la ayuda de canastas, y observamos que R. melanosterna y K. leucostomum fueron capturadas de esta manera. Las trampas de caída, cebadas con frutas, se utilizaron para capturar R. melanosterna durante incursiones a tierra firme. El "canasto tortuguero" es un cesto de fibra vegetal que tiene la parte interna en forma de embudo, construido con listones de madera (generalmente chonta) y en su exterior se atan dos trozos de madera de balsa para darle flotabilidad. Los canastos fueron cebados con plátanos u hojas de malanga (Xanthosoma) para atrapar especies de Rhinoclemmys y K. leucostomum. En las zonas pantanosas, se usó la técnica de tanteo o pisoteo para colectar R. melanosterna y K. leucostomum. La captura directa o a mano también es un método común de caza. Las tortugas fueron preparadas como alimento principalmente en sopas o guisos. El uso de las tortugas en la alimentación se ha generalizado en la región, posiblemente porque ha disminuido la cacería de animales grandes.