Urethral Injuries: Diagnostic and Management Strategies for Critical Care and Trauma Clinicians.

Urologic trauma is a well-known cause of urethral injury with a range of management recommendations. Retrograde urethrogram remains the preferred initial diagnostic modality to evaluate a suspected urethral injury. The management thereafter varies based on mechanism of injury. Iatrogenic urethral injury is often caused by traumatic catheterization and is best managed by an attempted catheterization performed by an experienced clinician or suprapubic catheter to maximize urinary drainage. Penetrating trauma, most commonly associated with gunshot wounds, can cause either an anterior and/or posterior urethral injury and is best treated with early operative repair. Blunt trauma, most commonly associated with straddle injuries and pelvic fractures, can be treated with either early primary endoscopic realignment or delayed urethroplasty after suprapubic cystostomy. With any of the above injury patterns and treatment options, a well thought out and regimented follow-up with a urologist is of utmost importance for accurate assessment of outcomes and appropriate management of complications.

First report of Cactodera milleri Graney and Bird, 1990 from Colorado and Minnesota.

In 2019, Cactodera milleri cysts were discovered from soil samples collected from a Chenopodium quinoa field, located in Mosca, Alamosa county, Colorado, USA. Approximately 200 lemon shaped cysts and several hundred juveniles were recovered from the affected quinoa plants. The same species was also identified from several counties in Minnesota from samples submitted over the years by the Minnesota Department of Agriculture as part of the Animal and Plant Health Inspection Service (APHIS) efforts to survey states for the presence of Pale Potato Cyst Nematode. The cysts and juveniles (J2) were recovered from soil samples through sieving and Baermann funnel extraction. The nematode species was identified by both morphological and molecular means as Cactodera milleri (Graney and Bird, 1990). To our knowledge this represents the first report of Cactodera milleri from Colorado and Minnesota.

Molecular and morphological characterization of the amaryllis lesion nematode, Pratylenchus hippeastri (Inserra et al., 2007), from California.

Root-lesion nematodes (Pratylenchus spp.) are among the most important nematode pests on grapevine along with root-knot, dagger, and ring nematodes. In 2019, two samples of both soil and roots were collected from a vineyard in Delano, Kern County, California and submitted to the United States Department of Agriculture, ARS, Mycology and Nematology Genetic Diversity and Biology Laboratory, Beltsville, MD, for identification purposes. Females and juveniles of Pratylenchus sp. were recovered from the root and soil samples using the sugar centrifugal flotation and Baermann funnel extraction methods. Both morphological observations and molecular analysis of internal transcribed spacer (ITS), 28S large subunit ribosomal DNA, and mitochondrial cytochrome oxidase (COI) sequences indicated that the specimens recovered from the soil and roots were Pratylenchus hippeastri. To the best of the authors' knowledge, this is the first report of P. hippeastri from California including the first record of this species on grapevine and the second state record in North America. Damages caused by nematodes cannot be over-emphasized, although economic importance of P. hippeastri has never been established. Hence, there is an urgent need to investigate the economic impact of this nematode in vineyards in California State in order to develop sustainable management strategies.Root-lesion nematodes (Pratylenchus spp.) are among the most important nematode pests on grapevine along with root-knot, dagger, and ring nematodes. In 2019, two samples of both soil and roots were collected from a vineyard in Delano, Kern County, California and submitted to the United States Department of Agriculture, ARS, Mycology and Nematology Genetic Diversity and Biology Laboratory, Beltsville, MD, for identification purposes. Females and juveniles of Pratylenchus sp. were recovered from the root and soil samples using the sugar centrifugal flotation and Baermann funnel extraction methods. Both morphological observations and molecular analysis of internal transcribed spacer (ITS), 28S large subunit ribosomal DNA, and mitochondrial cytochrome oxidase (COI) sequences indicated that the specimens recovered from the soil and roots were Pratylenchus hippeastri. To the best of the authors' knowledge, this is the first report of P. hippeastri from California including the first record of this species on grapevine and the second state record in North America. Damages caused by nematodes cannot be over-emphasized, although economic importance of P. hippeastri has never been established. Hence, there is an urgent need to investigate the economic impact of this nematode in vineyards in California State in order to develop sustainable management strategies.

Incidence and Risk Factors for Acute Kidney Injury in Severely Injured Patients Using Current Kidney Disease: Improving Global Outcomes Definitions.

BACKGROUND: Acute kidney injury (AKI) is a significant cause of morbidity and mortality for critically injured trauma patients. The Kidney Disease: Improving Global Outcomes (KDIGO) practice guideline is the most up-to-date classification for AKI. The aims of this study were to determine the incidence and risk factors for AKI in critically injured trauma patients using the current KDIGO definitions. STUDY DESIGN: A prospective cohort study was performed at our academic, level 1 trauma center, from September 2017 to August 2018. All adult trauma patients admitted to the surgical ICU were included. The primary outcome was the development of AKI, as defined by KDIGO. Secondary outcomes included hospital and ICU length of stay, ventilator days, and mortality. RESULTS: There were 466 patients included and 314 (67%) developed AKI. Those who developed AKI were more often hypotensive on admission (7% vs 2%), had higher Injury Severity Scores (ISS) (19 vs 13), were more likely to have severe injuries to the chest (40% vs 24%) and extremities (20% vs 6%), received transfusion (41% vs 21%), sustained crush injuries (8% vs 1%), received radiocontrast (75% vs 47%), nephrotoxic medication (74% vs 60%), or vasopressors (15% vs 3%). After multivariate analysis, risk factors independently associated with AKI include age, Injury Severity Score (ISS), severe extremity injuries, radiocontrast, and vasopressors. Those who developed AKI had higher mortality (9% vs 2%). CONCLUSIONS: Using current KDIGO criteria, the incidence of AKI in critically injured trauma patients was higher than previously reported. Older patients, with more severe injuries to their extremities and chest and who have suffered crush injuries, appear to be the most a risk. AKI in the critically injured patient results in an almost 5-fold increase in mortality.

Molecular and morphological characterization of the alfalfa cyst nematode, Heterodera medicaginis, from Utah.

In the spring of 2019, a cyst nematode was discovered from soil samples collected from an alfalfa field in Millard County, Utah. The soil samples were submitted to one of us (SH), who extracted the nematode cysts and sent them to the USDA-ARS, Mycology and Nematology Genetic Diversity and Biology Laboratory (MNGDBL), Beltsville, MD for morphological and molecular identification. Cysts and living nematode juveniles (J2) recovered from the cysts were examined morphologically and molecularly for species identification which indicated that the specimens were Heterodera medicaginis. This represents the first record of H. medicaginis in Utah and the second report of this nematode in North America.In the spring of 2019, a cyst nematode was discovered from soil samples collected from an alfalfa field in Millard County, Utah. The soil samples were submitted to one of us (SH), who extracted the nematode cysts and sent them to the USDA-ARS, Mycology and Nematology Genetic Diversity and Biology Laboratory (MNGDBL), Beltsville, MD for morphological and molecular identification. Cysts and living nematode juveniles (J2) recovered from the cysts were examined morphologically and molecularly for species identification which indicated that the specimens were Heterodera medicaginis. This represents the first record of H. medicaginis in Utah and the second report of this nematode in North America.

Metal organic framework-graphene nano-composites for high adsorption removal of DBT as hazard material in liquid fuel.

Nowadays, one of the most important energy issues is how to deal with the sulfur removal from liquid fuels in a simple and inexpensive ways. Adsorptive desulphurization process can solve this issue when use highly effective adsorbent like metal organic frameworks (MOFs) and its nano-composites. In this context, this study uses the green solvo-themal method for the preparation of MOF (Cu-BTC) and MOF/Graphene hybrid nano-composite to be used as an effective DBT adsorbent from the model fuels. The prepared adsorbents were characterized by using different analytical techniques like: XRD, FTIR, BET, and TEM. Adsorption experimental results demonstrated that the composite material MOF/Gr (9:1 wt ratio) exhibited extra ordinary high dibenzothiophene adsorption with adsorption capacity (46.2 mg S/g) under the optimum experimental conditions. Kinetic and Langmuir and Freundlich isotherm were also studied.

Multiple strategy peer-taught evidence-based medicine course in a poor resource setting.

BACKGROUND: Teaching Evidence Based Medicine (EBM) is becoming a priority in the healthcare process. For undergraduates, it has been proved that integrating multiple strategies in teaching EBM yields better results than a single, short-duration strategy. However, there is a lack of evidence on applying EBM educational interventions in developing countries. In this study, we aim to evaluate the effectiveness of a multiple strategy peer-taught online course in improving EBM awareness and skills among medical students in two developing countries, Syria and Egypt. METHODS: We conducted a prospective study with pre- and post- course assessment of 84 medical students in three universities, using the Berlin questionnaire and a set of self-reported questions which studied the students' EBM knowledge, attitude and competencies. The educational intervention was a peer-taught online course consisting of six sessions (90 min each) presented over six weeks, and integrated with assignments, group discussions, and two workshops. RESULTS: The mean score of pre- and post-course Berlin tests was 3.5 (95% CI: 2.94-4.06) and 5.5 (95% CI: 4.74-6.26) respectively, increasing by 2 marks (95% CI: 1.112-2.888; p-value <0.001), which indicates a statistically significant increase in students' EBM knowledge and skill, similar to a previous expert-taught face to face contact course. Self-reported confidences also increased significantly. However, our course did not have a major effect on students' attitudes toward EBM (1.9-10.8%; p-value: 0.12-0.99). CONCLUSION: In developing countries, multiple strategy peer-taught online courses may be an effective alternative to face to face expert-taught courses, especially in the short term.

Comparison Between Ground Ant (Hymenoptera: Formicidae) Communities Foraging in the Straw Mulch of Sugarcane Crops and in the Leaf Litter of Neighboring Forests.

In many sugarcane plantations in Brazil, the straw is left on the soil after harvesting, and vinasse, a by-product of the production of sugar and ethanol, is used for fertigation. Our goal was to compare ant community composition and species richness in the straw mulch of sugarcane crops with the leaf litter of neighboring forests. We tested the hypothesis that ant communities in the straw mulch of vinasse-irrigated sugarcane crops and in the forest leaf litter were similar, because the combination of straw mulching and vinasse irrigation has a positive effect on soil fauna. Straw mulch and leaf litter were collected from 21 sites and placed in Berlese funnels. In total, 61 species were found in the forest leaf litter, whereas 34 and 28 species were found in the straw mulch of sugarcane fields with and without vinasse, respectively. Ant communities differed between forest and crop fields, but the species in the sugarcane straw mulch were a subset of the species found in the forest leaf litter. Although vinasse is rich in organic matter, it did not increase ant diversity. Seven feeding and/or foraging types were identified and, among the different types, surface-foraging omnivorous ants were the most prevalent in all habitats. Vinasse-irrigated sugarcane straw mulch had more predatory species than mulch from vinasse-free fields, but fewer than forest leaf litter. However, this positive effect of vinasse irrigation should be carefully evaluated because vinasse has negative effects on the environment.

Vinasse and Its Influence on Ant (Hymenoptera: Formicidae) Communities in Sugarcane Crops.

Sugarcane is an important crop within the Brazilian socioeconomic landscape. There is a constant need for approaches to increase sustainability at all steps of the production chain. Irrigating sugarcane crops with vinasse is one of these approaches, because vinasse is a residue of sugarcane processing that can be used to fertilize these same crops. However, due to its chemical properties, vinasse may be harmful to soil fauna. Analyzing the structure and functional organization of ant communities is a fast and practical way to monitor sites affected by the addition of chemicals. This study compared the structure of soil ant communities in vinasse-irrigated sugarcane crops to those in secondary forests adjacent to the crops. In total, 32 genera and 107 species of ants were observed; of these, 30 species foraged in crop fields and 102 foraged in forests. Twenty-five percent of the species were present in both crops and forests. Ant communities in crop soil had poorer taxonomic composition and lower richness in each functional group compared to communities in forest remnants. However, regardless of vegetation type, epigeic ants were more diverse, and Dorymyrmex brunneus (crop) and Pachycondyla striata (forest) were very frequent. Vinasse did not increase the diversity of epigeic and hypogeic ants, but it may affect the community composition.

Mechanisms of bradykinin-induced expression of connective tissue growth factor and nephrin in podocytes.

Diabetic nephropathy (DN) is the main cause of morbidity and mortality in diabetes and is characterized by mesangial matrix deposition and podocytopathy, including podocyte loss. The risk factors and mechanisms involved in the pathogenesis of DN are still not completely defined. In the present study, we aimed to understand the cellular mechanisms through which activation of B2 kinin receptors contribute to the initiation and progression of DN. Stimulation of cultured rat podocytes with bradykinin (BK) resulted in a significant increase in ROS generation, and this was associated with a significant increase in NADPH oxidase (NOX)1 and NOX4 protein and mRNA levels. BK stimulation also resulted in a signicant increase in the phosphorylation of ERK1/2 and Akt, and this effect was inhibited in the presence of NOX1 and Nox4 small interfering (si)RNA. Furthermore, podocytes stimulated with BK resulted in a significant increase in protein and mRNA levels of connective tissue growth factor (CTGF) and, at the same time, a significant decrease in protein and mRNA levels of nephrin. siRNA targeted against NOX1 and NOX4 significantly inhibited the BK-induced increase in CTGF. Nephrin expression was increased in response to BK in the presence of NOX1 and NOX4 siRNA, thus implicating a role for NOXs in modulating the BK response in podocytes. Moreover, nephrin expression in response to BK was also significantly increased in the presence of siRNA targeted against CTGF. These findings provide novel aspects of BK signal transduction pathways in pathogenesis of DN and identify novel targets for interventional strategies.

Learning curve and early clinical outcomes for a robotic surgery novice performing robotic single site cholecystectomy.

BACKGROUND: A rapid training protocol has been developed for robotic surgery novices to learn robotic single-incision techniques. This study assesses the learning curve and early clinical results for a robotic surgery novice starting single-site cholecystectomy. METHODS: A chart review was performed on the surgeon's first 55 patients to undergo this procedure. RESULTS: Average patient age was 46.01 ± 4.25 (range 21-86) years and BMI was 26.57 ± 4.25 (range 19.4-36.6) kg/m(2) . The mean port placement with docking time was 11.34 ± 3.74 (range 7-23) min. Mean console time was 28.74 ± 11.04 (range 15-66) min. Average total OR time was 61.84 ± 14.66 (range 40-105) min. All procedures were successfully completed without conversion or added ports. Complications included several minor procedural gall bladder perforations and miscellaneous postoperative symptomatic complaints. CONCLUSION: Robotic single site cholecystectomy can be safely performed by a robotic novice within a minimal learning curve and have early clinical results that are comparable to the published data of robotic experts.

Bacterial contamination in intensive care unit at Al-Imam Al-Hussein Hospital in Thi-qar province in Iraq.

Cross- infection from patient to patient or from hospital personnel to patients represents constant hazards. It is one of the most important causes of morbidity and mortality especially in Intensive Care Unit all over the world. To identify the types and the source of bacterial contamination in ICU and to study the sensitivity of bacterial isolates to commonly used antibiotics in hospitals this study had been conducted in Al-Imam Al-Hussein hospital in Thi-qar province for the period from the 1st of September to the end of December 2011. A total of 320 swabs and samples were collected from 17 different sites of Intensive Care Unit environment and inoculated on a normal cultural media, then incubated at 37°C for 24 hour. The obtained growth revealed different bacterial colonies which had been tested for their morphological and biochemical characteristics. Sixty eight of pure isolates were obtained including 24 (35.29%) Gram positive bacterial isolates, and 44(64.71%) of Gram negative bacterial isolates, the highest rates (19.11%) of bacterial contamination had been found on the walls and the floor. Sensitivity tests for all isolates were done using 25 types of commonly used antibiotics in Iraq, among Gram negative bacteria and gram positive bacteria the genus Enterobacter spp and Staphylococcus spp respectively, showed the highest resistance to most of the tested antibiotics, MIC tests for 5 types of antibiotics being applied for the most resistant and the most sensitive isolates had identified that all isolates have a low rate of MIC against Ciprofloxacine. Bacillus spp and Enterobacter spp were the most prevalent bacterial contaminants of Intensive Care Unit environment .such contamination could be managed mostly by strict application of sterilization measures.

A prospective study of predictors of successful weight maintenance by women enrolled in community-based weight-loss programs.

OBJECTIVE: Research is limited on the psychological predictors of successful long-term weight maintenance after weight loss. Therefore, the present study examined the individual contributions of body image, depression, and self-esteem to successful weight maintenance after a significant weight loss. METHOD: Measures of depression, self-esteem, and body image were administered at baseline, three-, and twelve-months to 73 women enrolled in community- based weight loss programs. RESULTS: Significant predictors of weight maintenance included baseline self-classified weight, early (3 months) weight maintenance, later (12 months) change in perceptual body image, and the interaction between early and late change in body image avoidance. Classifying oneself as more overweight at baseline predicted less weight maintenance, while later discrepancies between current and realistic body size were positively associated with weight maintenance. Those who showed steady improvements in body image avoidance behavior were more likely to maintain weight loss. Baseline BMI, self-esteem, and depression did not predict 12-month weight maintenance. DISCUSSION: Further understanding how body image and related factors contribute to weight maintenance may inform development of more effective interventions to promote long-term weight maintenance.

Effects of collagen type on the behavior of adult canine annulus fibrosus cells in collagen-glycosaminoglycan scaffolds.

The healing of intervertebral disc defects may be improved by the implantation of cell-seeded collagen-based scaffolds. The present study evaluated in vitro the effects of the collagen type (type I vs type II) from which the scaffolds were fabricated on the behavior of adult canine annulus fibrosus cell-seeded collagen-glycosaminoglycan (GAG) scaffolds. Protein and GAG biosynthesis continued through the 8-week period of the experiment, demonstrating the viability of the cells in type I and type II collagen-GAG scaffolds. Statistical analysis revealed no significant effect of the type of collagen that makes up the scaffold on the biosynthetic activity. For both scaffold types, the amount of retained newly synthesized proteins increased from 1 to 2 weeks and from 6 to the 8 weeks. In contrast, the rate of GAG retention increased to a maximum at 4 weeks for both types of scaffolds, then decreased to about 50% of that level after 8 weeks. The number of cells generally increased the first week but then decreased in type I scaffolds while remaining constant in type II scaffolds. It could be assumed that most newly synthesized protein was lost to the medium, as the quantity of protein and collagen remained constant. Cell-mediated contraction of the scaffolds contributed to folds that formed in the constructs and to create an oriented architecture tissue. These findings commend the use of a type II collagen-GAG scaffold for further study on the basis of its maintenance of cell number and the slightly higher accumulated GAG content.

Specialized metabolism and biochemical suppression during aestivation of the extant South American lungfish -- Lepidosiren paradoxa

Lepidosiren paradoxa (pirambóia) is the single representative of Dipnoan (lungfish) in South America. This species is considered a living fossil, in spite of some reports describing this fish as having a very specialized life style. It aestivates during the dry season, and has developed metabolic adaptations to cope with both flooding and drought. The literature describing its tissue ultra-structure shows high glycogen stored in the muscle, suggesting a strong dependence on anaerobic glycolysis. The present paper reports tissue enzyme levels of LDH, MDH, and CS, and isozymic tissue distribution of LDH, MDH, ADH, PGI, SOD, and PGM of 7 aestivating specimens from Lago do Canteiro in the Amazonas River. Animals were caught while burrowed in mud during the aestivation period. Our findings reveal high anaerobic capacity of both skeletal and heart muscles, even during the aestivation period, when enzymes showed suppressed levels compared to those of non-aestivating animals (data from the literature). Isozymic patterns suggest loss of duplicate condition in most analyzed loci, a characteristic that occurs mainly in higher vertebrate categories. These data indicate that, compared to the fish group, lungfish may be considered advanced, despite retaining primitive morphological characters

Specialized metabolism and biochemical suppression during aestivation of the extant South American lungfish -- FONT FACE=Symbol> /FONT>Lepidosiren paradoxa

Lepidosiren paradoxa (pirambóia) is the single representative of Dipnoan (lungfish) in South America. This species is considered a living fossil, in spite of some reports describing this fish as having a very specialized life style. It aestivates during the dry season, and has developed metabolic adaptations to cope with both flooding and drought. The literature describing its tissue ultra-structure shows high glycogen stored in the muscle, suggesting a strong dependence on anaerobic glycolysis. The present paper reports tissue enzyme levels of LDH, MDH, and CS, and isozymic tissue distribution of LDH, MDH, ADH, PGI, SOD, and PGM of 7 aestivating specimens from Lago do Canteiro in the Amazonas River. Animals were caught while burrowed in mud during the aestivation period. Our findings reveal high anaerobic capacity of both skeletal and heart muscles, even during the aestivation period, when enzymes showed suppressed levels compared to those of non-aestivating animals (data from the literature). Isozymic patterns suggest loss of duplicate condition in most analyzed loci, a characteristic that occurs mainly in higher vertebrate categories. These data indicate that, compared to the fish group, lungfish may be considered advanced, despite retaining primitive morphological characters.

Lepidosiren paradoxa (pirambóia) é o único representante dos Dipnoan (peixes pulmonados) na América do Sul. Essa espécie é considerada um "fóssil vivo", apesar de alguns estudos terem revelado um estilo de vida muito especializado. A espécie pode ser encontrada estivando durante a seca e desenvolvendo adaptações metabólicas para enfrentar as trocas que ocorrem periodicamente em seu ambiente: inundações e secas. As descrições encontradas na literatura sobre as ultra-estruturas dos tecidos revelam alta concentração de glicogênio (substância de estocagem) no músculo, sugerindo forte tendência para a glicólise anaeróbica. O presente estudo descreve os níveis enzimáticos de LDH, MDH e CS nos tecidos do coração e músculo bem como a distribuição enzimática das isozimas LDH, MDH, ADH, PGI, SOD e PGM em 7 espécimes coletadas no Lago do Canteiro, Rio Amazonas, ocasião em que se encontravam estivando. Os animais foram capturados pelos pescadores (armadilha individual) durante o período em que estavam enterrados na lama do lago durante a "seca". Nossos resultados revelaram alta capacidade anaeróbica dos músculos esquelético e cardíaco no período, quando as enzimas mostram níveis bastante inferiores (indicando supressão metabólica) em relação aos animais ativos (dados obtidos na literatura). Os padrões isozímicos sugerem a perda da condição de loci duplicados na maioria dos sistemas isozímicos, característica que ocorre principalmente em vertebrados pertencentes a categorias superiores. Esses dados indicam que, comparado ao grupo dos peixes, o peixe pulmonado pode ser considerado especializado (ou avançado), apesar da manutenção de duas características morfológicas primitivas.

Specialized metabolism and biochemical suppression during aestivation of the extant South American lungfish--Lepidosiren paradoxa.

Lepidosiren paradoxa (pirambóia) is the single representative of Dipnoan (lungfish) in South America. This species is considered a living fossil, in spite of some reports describing this fish as having a very specialized life style. It aestivates during the dry season, and has developed metabolic adaptations to cope with both flooding and drought. The literature describing its tissue ultra-structure shows high glycogen stored in the muscle, suggesting a strong dependence on anaerobic glycolysis. The present paper reports tissue enzyme levels of LDH, MDH, and CS, and isozymic tissue distribution of LDH, MDH, ADH, PGI, SOD, and PGM of 7 aestivating specimens from Lago do Canteiro in the Amazonas River. Animals were caught while burrowed in mud during the aestivation period. Our findings reveal high anaerobic capacity of both skeletal and heart muscles, even during the aestivation period, when enzymes showed suppressed levels compared to those of non-aestivating animals (data from the literature). Isozymic patterns suggest loss of duplicate condition in most analyzed loci, a characteristic that occurs mainly in higher vertebrate categories. These data indicate that, compared to the fish group, lungfish may be considered advanced, despite retaining primitive morphological characters.

[Esophageal histoplasmosis. A case report]. / Histoplasmose do esôfago. Relato de caso.

The authors report a case of a patient with complaint of progressive disphagia. Stenoses of lower third of esophagus was revealed by radiological and endoscopic examinations. Fungi were showed in biopsy of lesion, with demonstration of Histoplasm capsulate by tissue culture. Endoscopic dilatation was performed because especific medical treatment failed but esophageal rupture was observed. Partial esophagectomy was performed with symptoms remission.

[Comparative characteristics of color reactions to ecdysteroids]. / Sravnitel'naia kharakteristika tsvetnykh reaktsii na ékdisteroidy.

The colour reactions used to determine sterols have been compared to find most sensitive method of quantitative determination of ecdysteroids. It is shown that the Liberman-Burchard reaction is most sensitive to most ecdysteroids, while the Chugaev reaction is most specific.

[Biochemical characterization of fractionated rat liver chromatin in experimental D-hypovitaminosis and after administration of steroidal drugs]. / Biokhimicheskie kharakteristiki fraktsionirovannogo khromatina pecheni krys v usloviiakh éksperimental'nogo D-gipovitaminoza i pri vvedenii preparatov steroidov.

Marked changes in the structural and functional characteristics of liver nuclear chromatin fractions are observed under experimental D-hypovitaminosis, which differ in the degree of transcriptional activity. DNA-polymerase activity and activity of the fraction, enriched with RNA-polymerase I, increases in the active fraction. Free radical LPO reactions are modified in the chromatin fraction with low activity and to the less degree in the active one. Disturbances of chromatine structural properties are caused with the change in the protein and lipid components of chromatin. Administration of ecdysterone preparations (separately and together with vitamin D3) has a partial corrective effect on structural and functional organization of nuclear chromatine. At the action of ecdysterone normalization of LPO reactions modified by pathological changes is observed in the chromatin fraction with low activity and to the less degree in the active one. This kind of influence corrects to the less degree chromatin functional activity and quantitative and qualitative modifications of its protein component. Simultaneous influence of ecdysterone and vitamin D3 leads to the partial normalization of the biochemical indices studied (except for those which characterize LPO reactions) mainly in the active chromatin fraction.