ABSTRACT
Sporadic colorectal cancer (CRC) is a consequence of the accumulation of genetic and epigenetic alterations that result in the transformation of normal colonic epithelial cells to adenocarcinomas. Studies have indicated that a common event in the tumorigenesis of CRC is the association of global hypomethylation with discrete hypermethylation at the promoter regions of specific genes that are involved in cell cycle regulation, DNA repair, apoptosis, angiogenesis, adhesion and invasion. The present study aimed to investigate the epigenetic changes (DNA methylation) in 24 candidate genes in CRC. A total of 10 candidate hypermethylated (HM) and unmethylated (UM) genes were identified that may be useful epigenetic markers for non-invasive CRC screening. The five genes that had the highest average UM percentages in the control group were MLH1 (71.7%), DKK2 (69.6%), CDKN2A (68.4%), APC (67.5%) and hsa-mir-342 (67.4%). RUNX3 (58.9%), PCDH10 (55.5%), SFRP5 (52.1%), IGF2 (50.4%) and Hnf1b (50.0%) were the five genes with the highest average HM percentages in the test group. In summary, the present preliminary study identified the methylation profiles of normal and cancerous colonic epithelial tissues, and provided the groundwork for future large-scale methylation studies.
ABSTRACT
The present study describes the technique and evaluation of postsurgical results, hospital stay, and follow-up of patients who underwent hepatic cyst surgical treatment by laparoscopic access. Twelve patients presenting liver cystic disease were included in this research. Clinical conditions, laboratorial and radiologic examinations, surgical technique, complications, and postoperative follow-up were discussed. In our series, the morbidity rate was 25%. No mortality was observed in the postoperative period. Conversion to open surgery was necessary in 1 case, owing to hemorrhage. No recurrence was found and patients remain asymptomatic in their current follow-up. Laparoscopic resection of hepatic cyst proved to be efficient in the regression of the cysts and resulted in disappearance of symptoms.
Subject(s)
Cysts/surgery , Laparoscopy/methods , Liver Diseases/surgery , Aged , Follow-Up Studies , Humans , Length of Stay , Middle Aged , Prospective Studies , Treatment OutcomeABSTRACT
Analisam-se os ressultados obtidos em 31 doentes operados por ulcera gástrica "alta". Discutem os autores, os pontos controversos em relaçao a terminologia, ao conceito e ao tratamento cirurgico dessas lesoes. Houve predominancia de sangramento digestivo como queixa principal (64,5%). Tanto o exame endoscopico quanto o histopatologico estao sujeitos a erros diagnosticos. Os indices de morbidade e de mortalidade estao relacionados a fatores como idade, molestias associadas e a maior ou menor extensao da ressecçao gastrica
