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1.
FEMS Immunol Med Microbiol ; 25(1-2): 155-65, 1999 Aug 01.
Article in English | MEDLINE | ID: mdl-10443504

ABSTRACT

Epidemiological studies indicate that breast-fed infants are at a decreased risk of sudden infant death syndrome (SIDS) compared to formula-fed infants. Increasing evidence suggests that infectious agents might be involved in some of these deaths, in particular bacteria which colonise mucosal surfaces and produce superantigenic toxins. One species implicated in recent studies of SIDS infants is Staphylococcus aureus. We tested the hypothesis that in comparison to infant formula, human milk might be a better inhibitor of binding of S. aureus to epithelial cells. In this study, two protocols were used for the binding assays which were assessed by flow cytometry: the in vitro method in which bacteria were treated with milk or formula, washed and added to epithelial cells; and a method more closely reflecting the competitive interactions in vivo in which cells, bacteria, and milk or infant formula were added at the same time. With the in vivo method, breast milk caused enhancement of bacterial binding to cells whilst infant formula caused inhibition; however, for the in vitro method, both human milk and infant formula caused consistent enhancement of binding. Flow cytometry and light microscopy studies indicated that the enhancement was due to the formation of bacterial aggregates. Human milk and infant formula preparations were also compared for components (antibodies or oligosaccharides) that could inhibit binding of S. aureus using the in vitro method. Human milk contained both IgA and IgG. Neither human milk nor infant formula contained oligosaccharides reactive with the Ulex europaeus lectin but both contained components that bound monoclonal antibodies to Lewis(a) and Lewis(b) antigens which can act as receptors for S. aureus. With both methods, synthetic Lewis(a) and Lewis(b) inhibited S. aureus binding in a dose-dependent manner. With human milk, however, the only component which showed a significant correlation with inhibition of binding was the IgA specific for the staphylococcal surface component that binds Lewis(a). Both human milk and infant formula contain components which could potentially inhibit bacterial binding but only breast milk contains the IgA specific for the bacterial adhesin that binds Lewis(a). Studies using the in vivo method suggest that protection associated with breast feeding in relation to SIDS could be due mainly to the formation of bacterial aggregates. The studies have implications for further research into constituents of infant formula.


Subject(s)
Breast Feeding , Epithelial Cells/microbiology , Infant Food , Milk, Human , Staphylococcus aureus/metabolism , Sudden Infant Death/prevention & control , Adult , Animals , Bacterial Adhesion , Enzyme-Linked Immunosorbent Assay , Female , Humans , Infant , Infant, Newborn , Lewis Blood Group Antigens/metabolism , Milk, Human/chemistry , Staphylococcus aureus/drug effects , Tumor Cells, Cultured
2.
FEMS Immunol Med Microbiol ; 25(1-2): 167-73, 1999 Aug 01.
Article in English | MEDLINE | ID: mdl-10443505

ABSTRACT

Breast feeding is known to protect an infant against gastrointestinal pathogens and epidemiological studies indicate that compared to breast fed infants, formula fed infants are at a greater risk of dying from sudden infant death syndrome (SIDS). Many SIDS infants have symptoms of gastrointestinal infections prior to death and one gastrointestinal pathogen associated with SIDS is Clostridium perfringens. Studies have found that a significantly higher number of formula fed SIDS infants have C perfringens and its enterotoxin in their faeces compared to breast fed infants. The aim of the study was to compare the effects of human milk and infant formula on binding of C perfringens to epithelial cells. Two protocols were used to assess the effect of human milk and infant formula to inhibit binding of C perfringens to epithelial cells. Binding was assessed by flow cytometry. For the in vivo protocol which more closely represents interactions on the mucosal surface, breast milk enhanced bacterial binding but infant formula caused inhibition of binding; however for the in vitro method, both human milk and infant formula resulted in consistent enhancement of binding. Flow cytometry studies indicated that enhancement of binding was due to the formation of bacterial aggregates. Lewis(a) and Lewis(b) antigens, found in both breast milk and infant formula, inhibited C. perfringens binding in a dose dependent manner. The Lewis(a) and Lewis(b) antigens in human milk and infant formula can inhibit C. perfringens binding to epithelial cells. While infant formula reduced binding of C. perfringens to epithelial cells in the experiments carried out with the in vivo protocol, the protective effects of breast feeding in relation to colonisation with C. perfringens are more likely to be due to formation of bacterial aggregates. These findings have implications for improving infant formula preparations.


Subject(s)
Breast Feeding , Clostridium perfringens/metabolism , Epithelial Cells/microbiology , Infant Food , Milk, Human , Sudden Infant Death/prevention & control , Animals , Bacterial Adhesion , Humans , Infant , Infant, Newborn , Lewis Blood Group Antigens/metabolism , Milk, Human/chemistry , Tumor Cells, Cultured
3.
FEMS Immunol Med Microbiol ; 25(1-2): 175-82, 1999 Aug 01.
Article in English | MEDLINE | ID: mdl-10443506

ABSTRACT

Two toxin-producing bacteria implicated in sudden infant death syndrome (SIDS) are Staphylococcus aureus and Clostridium perfringens. Epidemiological studies have shown that breast feeding reduces an infant's risk of SIDS. This protective effect could be due partly to IgA antibodies to these toxins in human milk. The aim of this work was to use a quantitative ELISA to determine levels of IgA antibodies that bound to toxic shock syndrome toxin (TSST-1), staphylococcal enterotoxin C (SEC) and C. perfringens enterotoxin A (CEA) in individual samples of human milk. All samples of milk tested contained IgA antibodies that bound to the bacterial toxins. For individual samples, IgA bound to TSST-1, SEC and CEA were in the range of 900-3100 ng ml(-1), 1000-3600 ng ml(-1) and 1000-4300 ng ml(-1) respectively. Isolation of S. aureus from mothers donating breast milk samples was used to determine if the presence of bacteria affected IgA levels which bound TSST-1 and SEC. For 3/5 samples with levels above the upper limit of the standard deviation (2375 ng ml(-1)) for IgA bound to TSST-1, S. aureus was isolated from the mother whilst 4/5 samples found to contain levels above the upper limit of the standard deviation (2627 ng ml(-1)) for IgA bound to SEC, had S. aureus isolated from the mother. In conclusion, if bacterial toxins do play a role in precipitating a SIDS death, the presence of IgA antibodies to toxins in breast milk, but not in infant formula, might contribute to the protective effect of breast feeding in relation to SIDS.


Subject(s)
Bacterial Toxins , Breast Feeding , Enterotoxins/immunology , Immunoglobulin A/analysis , Milk, Human/immunology , Sudden Infant Death/prevention & control , Superantigens , Adult , Animals , Antibodies, Bacterial/analysis , Antibodies, Bacterial/immunology , Clostridium perfringens/immunology , Clostridium perfringens/metabolism , Enzyme-Linked Immunosorbent Assay , Female , Humans , Immunoglobulin A/immunology , Infant , Infant Food , Infant, Newborn , Nose/microbiology , Pharynx/microbiology , Staphylococcus aureus/immunology , Staphylococcus aureus/isolation & purification , Staphylococcus aureus/metabolism
4.
FEMS Immunol Med Microbiol ; 23(2): 115-24, 1999 Feb.
Article in English | MEDLINE | ID: mdl-10076908

ABSTRACT

Respiratory virus infections have been suggested to be predisposing factors for meningococcal disease. Respiratory syncytial virus (RSV) affects young children in the age range at greatest risk of disease caused by Neisseria meningitidis. It has been previously shown that glycoprotein G expressed on the surface of RSV-infected HEp-2 cells (a human epithelial cell line) contributed to higher levels of binding of meningococci compared with uninfected cells. The aim of the present study was to examine the effect of RSV infection on expression of surface molecules native to HEp-2 cells and their role in bacterial binding. Flow cytometry and fluorescence microscopy were used to assess bacterial binding and expression of host cell antigens. Some molecules analysed in this study have not been reported previously on epithelial cells. RSV infection significantly enhanced the expression of CD15 (P < 0.05), CD14 (P < 0.001) and CD18 (P < 0.01), and the latter two contributed to increased binding of meningococci to cells but not the Gram-positive Streptococcus pneumoniae.


Subject(s)
Antigens, CD/metabolism , Bacterial Adhesion , HN Protein , Neisseria meningitidis/physiology , Respiratory Syncytial Viruses/physiology , Up-Regulation , Adhesins, Bacterial/immunology , Adhesins, Bacterial/metabolism , Animals , Antibodies, Monoclonal/pharmacology , Antigens, CD/immunology , Bacterial Adhesion/drug effects , CD18 Antigens/immunology , CD18 Antigens/metabolism , Cell Adhesion , Epithelial Cells , Erythrocytes/metabolism , Humans , Lewis X Antigen/immunology , Lewis X Antigen/metabolism , Lipopolysaccharide Receptors/immunology , Lipopolysaccharide Receptors/metabolism , Lipopolysaccharides/immunology , Lipopolysaccharides/metabolism , Neisseria meningitidis/drug effects , Sheep , Streptococcus pneumoniae/drug effects , Streptococcus pneumoniae/physiology , Tumor Cells, Cultured , Viral Envelope Proteins , Viral Proteins/analysis
5.
FEMS Immunol Med Microbiol ; 23(1): 27-36, 1999 Jan.
Article in English | MEDLINE | ID: mdl-10030544

ABSTRACT

Smoking is associated with an increased risk of respiratory tract infection in adults. In children, exposure to cigarette smoke is a risk factor for respiratory tract infection and bacterial meningitis: Active smoking and passive exposure to cigarette smoke is also associated with carriage of some potentially pathogenic species of bacteria in both adults and children. The aims of the study were to determine the effect of active smoking on: (1) bacterial binding to epithelial cells; (2) expression of host cell antigens that act as receptors for some species; and (3) the effects of passive exposure to water-soluble components of cigarette smoke on bacterial binding. Flow cytometry was used to assess binding to buccal epithelial cells of the following species labelled with fluorescein isothiocyanate: Neisseria meningitidis, Neisseria lactamica, Streptococcus pneumoniae, Bordetella pertussis, Haemophilus influenzae, Moraxella catarrhalis, Staphylococcus aureus. Flow cytometry was also used to assess expression of host cell antigens which have been identified as bacterial receptors. For each species, binding to cells of smokers was significantly higher than to cells of non-smokers; however, expression of host cell antigens was similar on epithelial cells of both groups. Non-dilute cigarette smoke extract reduced binding of bacteria to epithelial cells, but dilutions between 1 in 10 and 1 in 320 enhanced binding. We conclude that smokers might be more densely colonised by a variety of potentially pathogenic bacteria. The enhanced bacterial binding to epithelial cells of smokers is not related to enhanced expression of host cell antigens that can act as receptors for some species, but possibly to components in the smoke that alter charge or other properties of the epithelial cell surface. Passive coating of mucosal surfaces with components of cigarette smoke might enhance binding of potentially pathogenic bacteria.


Subject(s)
Bacterial Adhesion , Epithelial Cells/microbiology , Gram-Negative Bacteria/physiology , Gram-Positive Cocci/physiology , Mouth Mucosa/microbiology , Plant Lectins , Smoking , Adult , Animals , Antibodies, Monoclonal/immunology , Antigens, Surface/analysis , Flow Cytometry , Gram-Negative Bacteria/isolation & purification , Gram-Positive Cocci/isolation & purification , Humans , Infant , Lectins/metabolism , Mice , Mouth Mucosa/cytology
6.
FEMS Immunol Med Microbiol ; 16(1): 51-9, 1996 Nov.
Article in English | MEDLINE | ID: mdl-8954353

ABSTRACT

Asymptomatic infection due to Bordetella pertussis has been suggested to be one cause of sudden infant death syndrome (SIDS). We examined developmental and environmental factors previously found to affect binding of another toxigenic species, Staphylococcus aureus, to human epithelial cells: expression of the Lewis(a) antigen; infection with respiratory syncytial virus (RSV); exposure to cigarette smoke; and the inhibitory effect of breast milk on bacterial binding. Binding of two strains of B. pertussis (8002 and 250825) to buccal epithelial cells was significantly reduced by treating the cells with monoclonal antibodies to Lewis(a) (P < 0.05) and Lewis(x) (P < 0.01) antigens. Both strains bound in significantly greater numbers to cells from smokers compared with cells from non-smokers (P < 0.05). HEp-2 cells infected with RSV subtypes A or B had higher binding indices for both 8002 (P < 0.001) and 250825 (P < 0.01). On RSV-infected cells, there was significantly enhanced binding of monoclonal antibodies to Lewis(x) (P < 0.05), CD14 (P < 0.001) and CD18 (P < 0.01); and pre-treatment of cells with anti-CD14 or CD18 also significantly reduced binding of both strains of B. pertussis. Pre-treatment of the bacteria with human milk significantly reduced their binding to epithelial cells. The results are discussed in relation to our three-year survey of bacterial carriage among 253 healthy infants, their mothers and local SIDS cases between 1993-1995 and in relation to the change to an earlier immunisation schedule for infants and the recent decline in SIDS in Britain.


Subject(s)
Bacterial Adhesion , Bordetella pertussis/pathogenicity , Sudden Infant Death/etiology , Antibodies, Monoclonal/immunology , Bacteria/isolation & purification , CD18 Antigens/immunology , Carrier State/epidemiology , Carrier State/microbiology , Cells, Cultured , Epithelium/microbiology , Humans , Infant , Infant, Newborn , Lewis Blood Group Antigens/biosynthesis , Lewis Blood Group Antigens/immunology , Lipopolysaccharide Receptors/immunology , Milk, Human/immunology , Respiratory Syncytial Virus Infections/complications , Retrospective Studies , Smoking/adverse effects , Sudden Infant Death/epidemiology
8.
FEMS Immunol Med Microbiol ; 10(1): 25-30, 1994 Nov.
Article in English | MEDLINE | ID: mdl-7874075

ABSTRACT

Viral glycoproteins G and F are expressed on the surface of cells infected with respiratory syncytial virus (RSV). We investigated the role of these proteins in the previously reported enhanced binding of Neisseria meningitidis to RSV-infected HEp-2 cells. Virus particles attached to bacteria were detected by immunofluorescence with flow cytometry. Binding of FITC-labelled bacteria to RSV-infected cells was significantly inhibited by monoclonal antibody against glycoprotein G. Unlabelled bacteria interfered with binding of the anti-G monoclonal antibody to these cells. These interactions were not found with a monoclonal antibody against glycoprotein F. We propose that glycoprotein G of RSV expressed on the surface of infected cells might act as an additional receptor for meningococci.


Subject(s)
HN Protein , Neisseria meningitidis/metabolism , Respiratory Syncytial Viruses/physiology , Viral Fusion Proteins/physiology , Viral Proteins/physiology , Antibodies, Monoclonal/immunology , Antibodies, Viral/immunology , Bacterial Adhesion , Cell Line , Liver/cytology , Respiratory Syncytial Viruses/immunology , Viral Envelope Proteins
9.
FEMS Immunol Med Microbiol ; 9(2): 91-100, 1994 Aug.
Article in English | MEDLINE | ID: mdl-7804169

ABSTRACT

Epidemiological factors associated with susceptibility to respiratory infections are similar to those associated with Sudden Infant Death Syndrome. Here we review the evidence that respiratory pathogens might be involved in some cases of Sudden Infant Death Syndrome in the context of factors identified in epidemiological studies of cot deaths: the age range affected; mother' smoking; respiratory viral infections; immunisation status. Both laboratory and epidemiological evidence suggests that vulnerability of infants to infectious agents depends on interactions between genetic, developmental and environmental factors that contribute to colonisation by microorganisms, the inflammatory and specific immune responses and the infants' physiological responses to inflammatory mediators. A model is proposed to explain how microorganisms might trigger a series of events resulting in some of these unexpected deaths and discusses how the the present recommendations regarding child care practices might help reduce the numbers of Sudden Infant Death Syndrome cases associated with infectious agents.


Subject(s)
Bacterial Infections/complications , Sudden Infant Death/etiology , Virus Diseases/complications , Bacterial Toxins/toxicity , Humans , Immunization , Infant , Infant, Newborn , Smoking/adverse effects , Sudden Infant Death/prevention & control
10.
FEMS Immunol Med Microbiol ; 9(1): 15-21, 1994 Jun.
Article in English | MEDLINE | ID: mdl-7920460

ABSTRACT

There is evidence that the Lewis(a) blood group antigen is one of the receptors for a number of potentially pathogenic microorganisms. To determine how widely distributed the microbial adhesins are that bind this antigen, anti-idiotypic antibodies produced against monoclonal anti-Lewis(a) were used in coagglutination assays to screen a variety of species. The following were agglutinated: 7/7 strains of Staphylococcus aureus; 10/19 (53%) strains of Neisseria meningitidis; 8/13 (62%) strains of Haemophilus influenzae; 1/3 strains of Helicobacter pylori; 1/2 strains of Neisseria gonorrhoeae; 1/2 strains of Candida albicans. The application of the anti-idiotypic antibodies to studies of host cell receptors, isolation of adhesins and development of new epidemiological typing reagents is discussed.


Subject(s)
Adhesins, Bacterial/immunology , Antibodies, Anti-Idiotypic/biosynthesis , Lewis Blood Group Antigens/immunology , Agglutination Tests , Animals , Binding Sites, Antibody , Enzyme-Linked Immunosorbent Assay , Female , Mice , Mice, Inbred BALB C
11.
FEMS Immunol Med Microbiol ; 8(4): 315-20, 1994 May.
Article in English | MEDLINE | ID: mdl-8061655

ABSTRACT

A 67 kDa protein was isolated from cell membrane preparations of Staphylococcus aureus (NCTC 10655) by affinity adsorption with synthetic Lewis a antigen conjugated to Synsorb beads. Pre-treatment of buccal epithelial cells expressing Lewis a with the purified protein reduced binding of the staphylococcal strain to a greater extent than the material not bound to the Synsorb beads. The significance of this work is discussed with reference to expression of Lewis a antigen in infants and the proposed role of toxigenic strains of staphylococci in some cases of sudden infant death syndrome.


Subject(s)
Adhesins, Bacterial , Bacterial Adhesion/immunology , Bacterial Adhesion/physiology , Lewis Blood Group Antigens/metabolism , Staphylococcus aureus/immunology , Binding, Competitive , Cell Wall/chemistry , Cheek , Chromatography, Affinity , Electrophoresis, Polyacrylamide Gel , Humans , Infant , Mouth Mucosa/cytology , Sudden Infant Death/etiology
12.
Agents Actions ; 41(1-2): 108-10, 1994 Mar.
Article in English | MEDLINE | ID: mdl-7915870

ABSTRACT

Toxigenic bacteria such as Bordetella pertussis and Staphylococcus aureus have been implicated in some cases of sudden infant death syndrome (SIDS). We have previously demonstrated that the Lewis(a) antigen is an epithelial cell receptor for S. aureus, and this study demonstrated that Lewis(a) on human monocytes is also a receptor for staphylococcal enterotoxin B (SEB). Values obtained in assays for production of TNF-alpha and nitric oxide were greater for monocytes treated with SEB compared with those treated with lipopolysaccharide (LPS). Exposure to LPS increased the expression of Lewis(a) on monocytes. These results are discussed with reference to the reported enhancement of endotoxic shock by pyrogenic toxins.


Subject(s)
Antibodies, Monoclonal/pharmacology , Enterotoxins/metabolism , Lewis X Antigen/metabolism , Monocytes/immunology , Binding Sites , Enterotoxins/pharmacology , Humans , Monocytes/metabolism , Nitric Oxide/metabolism , Staphylococcus aureus , Tumor Necrosis Factor-alpha/metabolism
13.
Int J Legal Med ; 106(4): 205-8, 1994.
Article in English | MEDLINE | ID: mdl-8038113

ABSTRACT

Sudden Unexpected Nocturnal Deaths (SUND) occur in young, apparently healthy immigrant workers from Thailand, the Philippines and Bangladesh living among ex-patriot labour forces in countries such as Singapore and Saudi Arabia. Several factors associated with these deaths are similar to those observed for Sudden Infant Death Syndrome (SIDS): sleep related and mainly nocturnal occurrence; no prodromal illnesses other than mild respiratory tract infection; exposure to cigarette smoke; absence of invasive microorganisms at autopsy. The hypotheses proposed to explain these deaths in adults are examined. Based on our studies of the role toxigenic bacteria might play in some cases of SIDS, we suggest a new approach to the investigation of SUND.


Subject(s)
Death, Sudden/etiology , Emigration and Immigration , Ethnicity , Adult , Bacterial Infections/complications , Bacterial Infections/pathology , Bacterial Toxins/isolation & purification , Cause of Death , Death, Sudden/pathology , Female , Humans , Male , Risk Factors , Singapore , Thailand/ethnology , Tobacco Smoke Pollution/adverse effects
14.
Forensic Sci Int ; 61(2-3): 133-40, 1993 Oct.
Article in English | MEDLINE | ID: mdl-8307522

ABSTRACT

The ability of enzyme linked immunosorbent assays (ELISA) to detect Lewis and H antigens in secretions obtained from 280 autopsies was assessed. The ELISA results were compared with those for matched blood specimens examined by agglutination of erythrocytes by monoclonal anti-Lewis(a) and anti-Lewis(b) antibodies. There was good agreement between the results for the two tests and the ELISAs could be used to determine secretor status of the subject. While determination of ABO group with monoclonal anti-A and anti-B was possible even with lysed blood, the results for Lewis typing by erythrocyte agglutination were poor if the sample was lysed or partially lysed. Detection of the antigens by ELISA was as efficient among elderly subjects as among younger ones and both H and Lewis antigens could be detected on erythrocytes and in secretions up to 127 h after death.


Subject(s)
Agglutination Tests , Blood Grouping and Crossmatching/methods , Enzyme-Linked Immunosorbent Assay/methods , Forensic Medicine/methods , Lewis Blood Group Antigens/analysis , Mucus/chemistry , Saliva/chemistry , Adolescent , Adult , Age Factors , Aged , Aged, 80 and over , Evaluation Studies as Topic , Female , Humans , Male , Middle Aged , Penis , Time Factors , Vaginal Smears
15.
Epidemiol Infect ; 110(3): 507-17, 1993 Jun.
Article in English | MEDLINE | ID: mdl-8519316

ABSTRACT

Toxigenic strains of Staphylococcus aureus have been suggested to play a role in sudden infant death syndrome (SIDS). In this study we examined two factors that might enhance binding of toxigenic staphylococci to epithelial cells of infants in the age range in which cot deaths are prevalent: expression of the Lewis(a) antigen and infection with respiratory syncytial virus (RSV). By flow cytometry we demonstrated that binding of three toxigenic strains of S. aureus to cells from nonsecretors was significantly greater than to cells of secretors. Pre-treatment of epithelial cells with monoclonal anti-Lewis(a) or anti-type-1 precursor significantly reduced bacterial binding (P < 0.01); however, attachment of the bacteria correlated only with the amount of Lewis(a) antigen detected on the cells (P < 0.01). HEp-2 cells infected with RSV bound significantly more bacteria than uninfected cells. These findings are discussed in context of factors previously associated with SIDS (mother's smoking, bottle feeding and the prone sleeping position) and a hypothesis proposed to explain some cases of SIDS.


Subject(s)
Bacterial Adhesion/physiology , Epithelium/microbiology , Isoantigens/immunology , Lewis Blood Group Antigens/immunology , Respiratory Syncytial Viruses/physiology , Staphylococcus aureus/metabolism , Sudden Infant Death/etiology , Antibodies, Monoclonal , Cells, Cultured , Epithelial Cells , Humans , Infant
16.
Int J Legal Med ; 105(6): 333-8, 1993.
Article in English | MEDLINE | ID: mdl-8518199

ABSTRACT

Toxigenic bacteria have been implicated in some cases of Sudden Infant Death Syndrome (SIDS). Although there is not much evidence that Clostridia spp. are associated with SIDS in Britain, strains of Staphylococcus aureus producing pyrogenic toxins have been isolated from significant numbers of these infants at autopsy The pyrogenic toxins, produced by some strains of group A Streptococcus pyogenes as well as staphylococci, are powerful "superantigens" that have significant physiological effects including induction of fever > 38 degrees C. In this article, interactions between genetic and environmental factors that might enhance colonization of epithelial surfaces by toxigenic staphylococci are analyzed: infant's expression of Lewis(a) antigen which acts as a receptor for some microorganisms; viral infections; the effect of mother's smoking on susceptibility to respiratory infection. Based on epidemiological findings and laboratory investigations, a hypothesis is proposed to explain how bacteria producing pyrogenic toxins might contribute to some cot deaths.


Subject(s)
Bacterial Infections/pathology , Bacterial Toxins/analysis , Sudden Infant Death/pathology , Bacterial Infections/microbiology , Humans , Infant , Opportunistic Infections/microbiology , Opportunistic Infections/pathology , Risk Factors , Staphylococcal Infections/microbiology , Staphylococcal Infections/pathology , Streptococcal Infections/microbiology , Streptococcal Infections/pathology , Streptococcus pyogenes , Virus Diseases/microbiology , Virus Diseases/pathology
17.
J Clin Pathol ; 45(11 Suppl): 20-4, 1992 Nov.
Article in English | MEDLINE | ID: mdl-1474153

ABSTRACT

Because there is little evidence that invasive bacterial diseases contribute to cot deaths, most studies on infectious causes of SIDS have focused on viruses or toxin producing bacteria. Although epidemiological studies found marginally significant associations between influenza virus and SIDS, respiratory syncytial virus (RSV) was isolated from 90% of older infants with SIDS. There are conflicting reports that some toxigenic bacteria (Clostridium botulinum, Clostridium difficle, enterotoxigenic Escherichia coli and Staphylococcus aureus) might be implicated in cot deaths. S aureus are common micro-organisms and their toxins are very powerful. As the pyrogenic toxic shock syndrome toxin of S aureus can kill a previously healthy adult, it might easily kill a small infant. Based on our studies on susceptibility of infants to other infections, we suggest the following might be factors leading to colonisation of infants by toxin producing S aureus: The Lewis blood group antigen appears to act as a receptor for some micro-organisms. Epithelial cells expressing high concentrations of Lewis bound appreciably more toxin producing S aureus than cells expressing low concentrations of the antigen. Lewis is expressed in secretions of nearly 90% of infants aged 3 months, the peak age for SIDS. RSV infects about 50% of infants by the first year of life and it is often isolated from infants with SIDS. Studies in our laboratory indicate that RSV infected HEp-2 cells bind more toxin producing S aureus than uninfected controls.


Subject(s)
Bacterial Infections/complications , Sudden Infant Death/etiology , Virus Diseases/complications , ABO Blood-Group System/analysis , Disease Susceptibility , Humans , Infant , Infant, Newborn , Lewis Blood Group Antigens/analysis , Sudden Infant Death/blood
18.
Autoimmunity ; 7(4): 279-89, 1990.
Article in English | MEDLINE | ID: mdl-2102769

ABSTRACT

We have demonstrated that the inability to secrete the water soluble glycoprotein form of the ABO blood group antigens into saliva is significantly more common in patients with Graves' disease than control subjects (40% vs 27%: P less than 0.025) but not among those with Hashimoto's thyroiditis or spontaneous primary atrophic hypothyroidism. Non-secretion is associated with increased susceptibility to infection and to asymptomatic carriage of some microorganisms. Although Yersinia enterocolitica has been found to express antigen cross reactive with the TSH receptor, we did not find an increased prevalence of Yersinia species in the faeces of 107 patients with Graves' disease. The isolation rate (less than 1%) was similar to that observed in the local population with diarrhoeal illness. Salivary IgA levels determined by whole cell ELISA with Y. enterocolitica 03 were not elevated in the majority of specimens examined. The results suggest that in contrast to reports from Scandinavia, there is no strong evidence that yersiniae play a role in the pathogenesis of Graves' disease among patients in South east Scotland. Non-secretors are significantly over represented among patients with several other autoimmune diseases; however, with the exception of antitubulin antibodies, non-secretors with Graves' disease did not have more antibodies to other human antigens than secretor patients.


Subject(s)
Autoantibodies/analysis , Autoimmune Diseases/etiology , Glycoproteins/metabolism , Graves Disease/etiology , Yersinia Infections/immunology , Yersinia enterocolitica , ABO Blood-Group System/immunology , Adult , Antibodies, Bacterial/analysis , Autoimmune Diseases/immunology , Cross Reactions , Female , Glycoproteins/immunology , Graves Disease/immunology , Humans , Immunoglobulin A/analysis , Lewis Blood Group Antigens/immunology , Male , Middle Aged , Saliva/immunology
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