[Glutamate dehydrogenase activity in the pancreatic tissue in acute experimental pancreatitis and under the action of sodium thiosulphate]. / Aktivnost' glutamatdegidrogenazy v tkani podzheludochnoi zhelezy pri ostrom éksperimental'nom pankreatite i pod vliianiem tiosul'fata natriia.

It has been established that the development of acute pancreatitis is accompanied by the reduced activity of glutamate dehydrogenase in the mitochondrial fraction of pancreas, pronounced in the focus of tissue necrosis and less expressed in the reactive inflammation focus. Besides this in the pancreas redistribution of enzyme, activity in the subcellular organelles takes place and enzyme activity emerges in the cytosol and further--in the blood and peritoneum liquid. Sodium thiosulfate has a marked correlation effect.

[Diagnostic importance of determining leucine aminotransferase activity in acute pancreatitis]. / Diagnosticheskoe znachenie opredeleniia aktivnosti leitsin-aminotransferazy pri ostrom pankreatite.

It has been experimentally and clinically established that the determination of leucine-aminotransferase activity in the blood serum and abdominal exudate may serve as a marker for the early determination of pancreonecrosis and edematous (serous) pancreatitis.

[Hexokinase and lactate dehydrogenase activity and isoenzymatic makeup of the smooth gastric muscle in intact and sympathectomized rabbits]. / Aktivnost' i izofermentnyi sostav geksokinazy i laktatdegidrogenazy gladkoi myshtsy zheludka intaktnykh i desimpatizirovannykh krolikov.

After immunological sympathectomy in rabbits activity and isoenzyme spectra of hexokinase (HK) and lactate dehydrogenase (LDH) were studied in smooth muscle of stomach. Activity of KH in the muscles was statistically significant due to increase in content of a single isoenzyme of HK occurring in the tissue. Activity o LDH was unaltered. However the isoenzyme apectrum of the enzyme was changed: content of M-subunits was decreased and the content of H-subunits was increased. These alterations may lead to an increase in redox potential in cells of the tissues.

[Induction of experimental allergic encephalomyelitis with encephalitogenic myelin proteins from the spinal cord of a bull]. / Induktsiia éksperimental'nogo allergicheskogo éntsefalomielita éntsefalitogennymi belkami mielina iz spinnogo mozga byka.

A homogenous base protein, exhibiting high encephalitogenic activity towards guinea pigs and rabbits, was isolated from bovine spinal cord using column chromatography. Administration of the preparation obtained together with Freund's adjuvant into the animals caused development of neurogic symptoms typical for experimental allergic encephalomyelitis as well as formation of demyelinization foci both in spinal cord and in brain of guinea pigs and rabbits.

[Hexokinase and lactate dehydrogenase activity and isoenzymatic makeup of the soluble fraction of dissimilarly functioning rabbit muscles normally and in experimental allergic encephalomyelitis]. / Aktivnost' i izofermentnyi sostav geksokinazy i laktatdegidrogenazy rastvorimoi fraktsii razlichno funktsioniruiushchikh myshts krolika v norme i pri éksperimental'nom allergicheskom éntsefalomielite.

Only one isozyme of hexokinase (type I) was found in a soluble fraction of smooth muscle of rabbit stomach using column chromatography and polyacrylamide gel electrophoresis. By the pattern of metabolism the smooth muscle of rabbit stomach accupies an intermediate position between rapidly and slowly contracting sceletal muscles, approaching to musculus soleus by the activity and isozyme spectra of hexokinase and lactate dehydrogenase (LDH). Activity of these enzymes was altered not uniformly in dissimilarly functioning muscles of rabbits with experimental allergic encephalomyelitis: it was increased in musculus gastrocnemius of rabbits and decreased in soleus or in smooth muscles. LDH isoenzyme spectra changed towards an increase in aerobic H-subunits and decrease in anaerobic M-subunits in the soluble fraction of musculus gastrocnemius of rabbits with experimental allergic encephalomyelitis. Content of LDH-5 and LDH-4 was about 2-fold increased and content of LDH-1 and LDH-2 was decreased in musculus soleus.

[Activity and isoenzyme composition of the hexokinase and lactate dehydrogenase of different rabbit muscles]. / Aktivnost' i izofermentnyi sostav geksokinazy i laktatdegidrogenazy razlichnykh myshts krolika

The hexokinase activity (HK) exceeded several times and the lactate dehydrogenase activity (LDH) was lower in soluble fraction of rabbit slow sceletal muscle as compared with fast sceletal muscle. In the soluble fraction of stomach smooth muscle the HK activity was higher and the LDH activity--lower than in slow sceletal muscle. The LDH isozyme spectrum of soduble fraction of smooth muscle was similar to the spectrum of slow muscle (mobile H-forms prevailed). Muscles of pyloric and fundal stomach parts differed in the enzymes activity and their isozyme content.