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Curr Microbiol ; 66(2): 197-204, 2013 Feb.
Article in English | MEDLINE | ID: mdl-23129345

ABSTRACT

Aeromonas P2973 was isolated from the water of a brook in a cavern in the Czech Republic. This isolate could not be biochemically identified at the species level, considering all updated species descriptions. Subsequent extensive phenotypic characterisation, DNA-DNA hybridisation, 16S rRNA gene sequencing and a Multi-Locus Phylogenetic Analysis (MLPA) of the concatenated sequence of 7 housekeeping genes (gyrB, rpoD, recA, dnaJ, gyrA, dnaX and atpD; 4705 bp) was employed in an attempt to ascertain the taxonomy of this isolate. Based on this polyphasic approach, we describe a novel species of the genus Aeromonas, for which the name Aeromonas cavernicola sp. nov. is proposed, with strain CCM7641(T) (DSM24474(T), CECT7862(T)) as the type strain.


Subject(s)
Aeromonas/classification , Aeromonas/isolation & purification , Fresh Water/microbiology , Aeromonas/genetics , Aeromonas/physiology , Bacterial Proteins/genetics , Bacterial Typing Techniques , Caves , Cluster Analysis , Czech Republic , DNA, Bacterial/chemistry , DNA, Bacterial/genetics , DNA, Ribosomal/chemistry , DNA, Ribosomal/genetics , Microscopy, Electron, Transmission , Molecular Sequence Data , Molecular Typing , Nucleic Acid Hybridization , Phylogeny , RNA, Ribosomal, 16S/genetics , Sequence Analysis, DNA
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