Genomic features of Pseudomonas sp. RGM2144 correlates with increased survival of rainbow trout infected by Flavobacterium psychrophilum.

This study evaluated the probiotic potential of the biofilm formed by the strain Pseudomonas sp. RGM2144 on rainbow trout survival. When challenged with the fish pathogen Flavobacterium psychrophilum, Pseudomonas sp. RGM2144 increased rainbow trout survival to 92.7 ± 1.2% (control: 35.3 ± 9.5%, p < .0001). The draft genome of Pseudomonas sp. RGM2144 is 6.8 Mbp long, with a completeness 100% and a contamination of 0.4%. The genome contains 6122 protein-coding genes of which 3564 (~60%) have known functions. The genome and phylogeny indicate that Pseudomonas sp. RGM2144 is a new species in the Pseudomonas genus, with few virulence factors, plasmids, and genes associated with antimicrobial resistance, suggesting a non-pathogenic bacterium with protective potential. In addition, the genome encodes for 11 secondary metabolite biosynthetic gene clusters that could be involved in the inhibition of F. psychrophilum. We suggest that Pseudomonas sp. RGM2144 may be applied as a probiotic in salmonid fish farming.

Influence of organic matter and CO2 supply on bioremediation of heavy metals by Chlorella vulgaris and Scenedesmus almeriensis in a multimetallic matrix.

This research evaluated the influence of organic matter (OM) and CO2 addition on the bioremediation potential of two microalgae typically used for wastewater treatment: Chlorella vulgaris (CV) and Scenedesmus almeriensis (SA). The heavy metal (HM) removal efficiencies and biosorption capacities of both microalgae were determined in multimetallic solutions (As, B, Cu, Mn, and Zn) mimicking the highest pollutant conditions found in the Loa river (Northern Chile). The presence of OM decreased the total biosorption capacity, specially in As (from 2.2 to 0.0 mg/g for CV and from 2.3 to 1.7 mg/g for SA) and Cu (from 3.2 to 2.3 mg/g for CV and from 2.1 to 1.6 mg/g for SA), but its influence declined over time. CO2 addition decreased the total HM biosorption capacity for both microalgae species and inhibited CV growth. Finally, metal recovery using different eluents (HCl, NaOH, and CaCl2) was evaluated at two different concentrations. HCl 0.1 M provided the highest recovery efficiencies, which supported values over 85% of As, 92% of Cu, and ≈100% of Mn and Zn from SA. The presence of OM during the loaded stage resulted in a complete recovery of As, Cu, Mn, and Zn when using HCl 0.1 M as eluent.

Comparative uptake study of arsenic, boron, copper, manganese and zinc from water by different green microalgae.

This work represents a comparative uptake study of the toxic elements arsenic, boron, copper, manganese and zinc in monometallic and multimetallic solutions by four green microalgae species (Chlamydomonas reinhardtii, Chlorella vulgaris, Scenedesmus almeriensis and an indigenous Chlorophyceae spp.), evaluating the effect of pH and contact time. Maximum removal efficiencies for each toxic element were 99.4% for Mn (C. vulgaris, pH 7.0, 3 h), 91.9% for Zn (Chlorophyceae spp., pH 5.5, 3 h), 88% for Cu (Chlorophyceae spp., pH 7.0, 10 min), 40.7% for As (S. almeriensis, pH 9.5, 3 h) and 38.6% for B (S. almeriensis, pH 5.5, 10 min). B removal efficiencies decreased remarkably in multimetallic solutions (down to 0.2% in C. reinhardtii), except for Chlorophyceae spp., the only species isolated from a polluted environment. FTIR spectra shown the highest interactions for As (1150-1300 cm-1) and Cu (3300, 1741, 1535, 1350-1400 cm-1). Results confirm microalgae biomass as a potential biosorbent for toxic elements.

Correlación de ansiedad y contaminación acústica en los pacientes hospitalizados del hospital Almanzor Aguinaga Asenjo, junio 2010 / Correlation of anxiety and acoustic contamination in hospitalized patients of the hospital Almanzor Aguinaga Asenjo, june 2010

Objetivo: Correlacionar la ansiedad y la contaminación acústica en los pacientes hospitalizados del Hospital Almanzor Aguinaga Asenjo, Junio de 2010. Metodología: diseño descriptivo, correlaciones.Mediciones : en total 325 encuestas y 24 mediciones de ruido utilizando un sonómetro. Intervenciones: Se encuestó a pacientes hospitalizados, usando la escala de ansied ad estado-rasgo de Spielberger (test STAI) durante 8 días, realizándose 3 mediciones por día, 2 en horas puntas y 1 en hora no punta. Para el análisis estadístico se utilizó el coeficiente de correlación de Pearson. Principales medidas de resultados: ansiedad estado. Resultados: La ansiedad y la contaminación acústica tienen una correlación fuerte de 0,807. Al realizarse las mediciones de la contaminación acústica tanto en el 2do como en el 5to piso se logró determinar un promedio entre ambos de 64,05 (+5,78 dB). En el segundo piso se encontró mediciones en un rango de 53,4 dB a 76,5 dB. En el 5°piso de 54,5 dB a 76,5 dB, además cabe recalcar que es en este piso donde se encontró mayor número de mediciones elevadas. Según las mediciones realizadas en niveles de ansiedad estado en los pacientes del 2°y 5°piso, se pudo determinar que de las 48 mediciones realizadas, 6 de ellas presentan un nivel de ansiedad estado bajo y 42 medio. La ansiedad rasgo en las 48 mediciones se pudo determinar que el nivel fue en general medio. Conclusiones: Existe una correlación fuerte positiva entre la ansiedad y la contaminación acústica en lospacientes hospitalizados del Hospital Nacional Almanzor Aguinaga Asenjo.(AU)

Spontaneous coronary artery dissection: a rare etiology of ST elevation myocardial infarction.

Spontaneous coronary artery dissection (SCAD) is an extremely rare etiology of acute myocardial infarction, most commonly seen in pre-menopausal women with no risk factors for atherosclerotic coronary artery disease (CAD). We present the case of a 41-year-old woman with no history of systemic illness who presented severe, oppressive retrosternal pain with ST-segment elevations in leads I, AVL, V1 through V6 in the absence of risk factors for CAD. Emergency percutaneous coronary intervention (PCI) revealed an aneurysmatic dilatation of the left main coronary artery with complete dissection of the left anterior descending artery and the ramus branch, causing distal occlusion of the affected vessels. The patient was submitted for emergency Coronary Artery Bypass Graft (CABG) surgery. This is thought to be caused by hormonal changes that cause weakness of the vasa vasorum and promote hemorrhage into the media.

Outcomes after heart valve replacement surgery at the Cardiovascular Center of Puerto Rico and the Caribbean.

BACKGROUND: Heart valve surgery is the second most common type of cardiac surgery. However, there is limited information about the outcomes associated to these types of surgeries. METHODS: A retrospective review was performed in 91 Puerto Rican patients who underwent elective heart valve replacement surgery at the Cardiovascular Center of Puerto Rico and the Caribbean (CCPRC) between January 2004 and January 2005. Demographic features, comorbidities, electrocardiographic findings, surgical data, length of hospitalization, and associated in-hospital complications were determined. Data were examined using Fisher's exact test, chi-square test, analysis of variance and student t test to analyze differences between the study groups. RESULTS: The mean age of the study population was 61.1 +/- 13.2 years; 48 patients (52.7%) were males. Sixty-one patients (67.0%) had aortic valve replacement whereas thirty patients (33.0%) had mitral valve replacement. Patients with aortic valve replacement were older (63.1 +/- 13.5 years vs. 56.0 +/- 11.2 years, p < 0.05) and more likely to present left ventricular hypertrophy (57.4% vs. 16.7%, p < 0.05) than patients with mitral valve replacement. On the other hand, patients with mitral valve replacement presented more frequently atrial fibrillation (43.3% vs. 9.8%, p < 0.05) than those with aortic valve replacement. The estimated in-hospital mortality rate was 1.1%. CONCLUSIONS: A review of heart valve replacement surgery conducted between January 2004 and January 2005 at the CCPRC revealed that the in-hospital mortality rates, complications, surgical times, and length of hospitalization at the CCPRC compares favorably with those reported in the literature.

Comparación entre la prueba Abbott Plus-EIA y la prueba rápida DETERMINE, para el diagnóstico de HIV-1-HIV-2 en donantes y gestantes en el Hospital II Chocope-EsSALUD / Comparison between test-EIA Abbott Plus and DETERMINE rapid test for the diagnosis of HIV-1-HIV-2 in donors and pregnant women in the Hospital II-EsSALUD Chocope

El presente estudio estuvo dirigido con la finalidad de hacer la comparación entre la prueba Abbott Plus EIA y la Prueba DETERMINE, para el diagnóstico de HIV-1 HIV-2 en donantes y gestantes procedentes del Hospital II Chocope-EsSALUD del departamento La libertad Perú. Se analizaron 471 muestras de las cuales: 169 fueron de donantes y 302 fueron de gestantes. Siendo el grupo etáreo mas estudiado de 20 a 29 años. Según lo obtenido se observó 0% de positividad en las gestantes y tres casos en los donantes. La distribución de los donantes sometidos a los tests se observó una sensibilidad de 100%, especificidad de 88.8% y Valor predictivo de 66.70% para la prueba de Abbott Plus EIA. La distribución de gestantes sometidos a los tests se determinó una especificidad de 100% en la prueba de Abbott Plus EIA. Se puede concluir que: La prueba Abbott Plus EIA es más eficiente con una sensibilidad y especificidad mayor que la prueba rápida Determine. La prueba Abbott Plus EIA se considera que no tiene errores falsos positivos con interacciones inmunológicas.

The study was carried out in Chocope Hospital EsSALUD Peru. To compare between Abbott Plus EIA test and Determine test to determined HIV-1 and HIV-2 in donors and pregnancy women. We analyzed 471 samples in which we analyzed 169 donors and 302 pregnancy women. We observed that the persons who had 20 to 29 years old were the more representative. We observed 0% inpregnancy women and 3 cases of donors. In donors we observed the sensitivity of 100%, 88.8 % of specificity and predictive value was 66,7% to the Abbott Plus EIA test. We concluded that the Abbott plus EIA test was the most efficient with a specificity and sensibility more important than the Determine test.