Spilanthol: occurrence, extraction, chemistry and biological activities

Abstract Spilanthol (C14H23NO, 221.339 g/mol) is a bioactive compound that is found in many different plants that are used as traditional remedies throughout the world. It is present in Heliopsis longipes and several species in the genus Acmella, including A. oleracea L., also known as paracress and jambu. Its leaves and flowers have sensory properties (pungency, tingling, numbing, mouth-watering) that make it a popular spice and ingredient in several Brazilian dishes. Spilanthol can exert a variety of biological and pharmacological effects including analgesic, neuroprotective, antioxidant, antimutagenic, anti-cancer, anti-inflammatory, antimicrobial, antilarvicidal and insecticidal activities. So, the aim of this review is to present a literature review on the spilanthol that describes its occurrence, chemistry, extraction and biological activities.

Anion composition of açaí extracts.

Many products labeled açaí are presently marketed as natural supplements with various claimed health benefits. Authentic açaí is expensive; as a result, numerous products labeled as containing açaí are being sold that actually contain little or no açaí. Authentic açaí samples from Brazil and Florida as well as several reputed açaí products were analyzed by suppressed conductometric anion chromatography. Columns with different selectivities were used to obtain a complete separation of all anions. Tandem mass spectrometry was used for confirmation of the less common ions. Quinate, lactate, acetate, formate, galacturonate, chloride, sulfate, malate, oxalate, phosphate, citrate, isocitrate, and myo-inositol hexakisphosphate (phytate) were found. Only the Florida açaí had detectable levels of hexanoate. No açaí sample had any detectable levels of tartrate, which is present in abundance in grape juice, the most common adulterant. The highly characteristic anion profile and in particular the absence of tartrate can readily be used to identify authentic açaí products. Açaí from Florida had a 6 times greater level of phytate. The present analytical approach for phytate may be superior to extant methods.

Sensorial evolution of cassava flour (Manihot esculenta crantz) added to protein concentrate cassava leaves.

Cassava is regarded as the nutritional base of populations in developing countries, and flour, product made of cassava, is the most consumed in the world. The cassava leaves are very rich in vegetable proteins, but a big amount is lost in processing the crop. The objective of this study was to do a sensory evaluation of cassava flour to which a protein concentrate obtained from cassava leaves (CPML) was added. The CPML was obtained from cassava leaves by isoelectric precipitation and added to cassava paste for preparation of flour in three parts 2.5, 5, and 10%. The acceptance test was done by 93 consumers of flour, using hedonic scale of 7 points to evaluate characteristics like color, scent, flavor, bitterness, texture, and overall score. By the method of quantitative descriptive analysis (QDA), eight trained tasters evaluated the following characteristics: whitish color, greenish color, cassava flavor, bitter flavor, characteristic flavor, lumpiness, raw texture, leaf scent, and cassava scent. The acceptability test indicated that flour cassava with 2.5 was preferred. Whitish color, greenish color, cassava flavor, bitter flavor, salty flavor, characteristic flavor, lumpiness texture, raw texture, and the smell of the leaves and cassava flour were the main descriptors defined for flour cassava with CPML has better characteristics.

Effect of the yellow passion fruit peel flour (Passiflora edulis f. flavicarpa deg.) in insulin sensitivity in type 2 diabetes mellitus patients.

BACKGROUND: A study with the yellow passion fruit peel flour showed positive action in blood glucose control as therapies' adjuvant in patients with type 2 diabetes mellitus. Therefore, we evaluated its effect on insulin sensitivity since there is a quest for studies that focus at better understanding of insulin resistance aspects in diabetic patients. Furthermore its relationship with chronic complications can also give good prospects for alternative treatments. METHODS: A total of 43 type 2 diabetes volunteers (28 females and 15 males) ingested 30 g/day of the yellow passion fruit peel flour for two months. The levels of blood glucose and fasting insulin, HOMA index and glycated hemoglobin were measured for each patient before and after dietary supplementation. RESULTS: There was a significant difference in the fasting blood glucose values (P = 0.000) and glycated hemoglobin (P = 0.032) after supplementation. It was also seen a reduction in HOMA IR (P = 0.005) in the supplemented group, however it was not observed changes in insulin values for females. HOMA beta (P = 0.000) showed significant increase in its values for the studied group. CONCLUSIONS: The supplementation used decreased insulin resistance in type 2 diabetic patients, suggesting a positive action in blood glucose control as adjuvant therapy in conventional treatments.

Efeito da farinha da casca do maracujá-amarelo (Passiflora edulis f. flavicarpa Deg.) nos níveis glicêmicos e lipídicos de pacientes diabéticos tipo 2 / Effect of the flour of the yellow passion fruit peel (Passiflora edulis f. flavicarpa Deg.) in the glycemic and lipid levels of type 2 diabetes patients

A suplementação da dieta com fibras solúveis pode ser considerada uma importante medida terapêutica no tratamento de pacientes diabéticos e obesos. Para avaliar o efeito da farinha da casca de maracujá amarelo rica em pectina, foi realizado um ensaio clínico fase II com 43 pacientes portadores de Diabetes Mellitus tipo 2. Estes receberam diariamente 30 g do produto testado durante 60 dias. Observou-se diferença estatística significante na glicemia de jejum (p = 0,000) acompanhada pela redução nos valores médios da hemoglobina glicada (p = 0,032). Em relação ao perfil lipídico, não foi verificado redução dos níveis de colesterol total e colesterol LDL nos pacientes ao longo deste estudo; entretanto, houve redução nos níveis de Triglicerídeos e aumento do colesterol HDL nos mesmos. Os níveis glicêmicos apresentados pelos pacientes antes e após o uso da farinha da casca do maracujá são compatíveis com uma ação positiva no controle da glicemia como adjuvante das terapias convencionais.

The supplementation of diet with soluble dietary fiber can be considered an important therapy measure in the treatment of diabetic and obese patients. In order to evaluate the effect of the flour of the yellow passion fruit peel which is rich in pectin, a phase II clinical trial with 43 patients with type 2 Diabetes Mellitus was performed. They received daily 30 g of the tested product for 60 days. Statistically significant difference was observed in fasting plasma glucose (p = 0.000) accompanied by a reduction in the average values of glycated hemoglobin (p = 0.032). In relation to the lipid profile, there were no reduced levels of total cholesterol and LDL-cholesterol in patients during this study; however, there were reduction in the levels of Triglycerides and increase in HDL cholesterol in them. The glycemic levels presented by the patients before and after the use of the passion fruit peel flour are compatible to a positive action to control blood glucose as an adjunct of conventional therapies.

Uso de Passiflora edulis f. flavicarpa na redução do colesterol / Use of Passiflora edulis f. flavicarpa on cholesterol reduction

A farinha da casca de maracujá é rica em uma fibra solúvel chamada pectina. Para avaliar o efeito dessa farinha na redução do colesterol foi realizado um estudo clínico piloto com dezenove mulheres, com idade entre 30 e 60 anos e apresentando hipercolesterolemia (colesterol > 200 mg/dL). Elas foram tratadas diariamente com 30 g da farinha da casca de maracujá por 60 dias. Após esse tempo foi observada uma redução estatisticamente significante (p < 0,05) nos níveis colesterol total (p = 0,00000) e colesterol LDL (p = 0,01193). Os resultados sugerem que a farinha da casca de maracujá seja utilizada na alimentação humana, juntamente com os alimentos, ou como matéria prima na produção de outros produtos, com o objetivo de reduzir o colesterol.

The flour of the passion fruit peel is rich in a soluble fiber called pectin. In order to evaluate the effect of this flour on cholesterol reduction, a pilot clinical study, was carried out with nineteen women, aged between 30 and 60 years, all of whom presented high cholesterol level (cholesterol > 200 mg/dL). The treatment consisted of daily doses of 30 g of the flour during 60 days. At the end of the 60 days it was observed a statistical reduction (p < 0.05) of the total cholesterol (p = 0,00000) and LDL-cholesterol (p = 0,01193) levels. The results suggested that the passion fruit peel flour, rich in pectin, can be used in human diet, with a large variety of foods, or as a raw material for other products intending to reduce cholesterol levels.