Enhancement of the Therapeutic Effect of Albendazole on Cystic Echinococcosis using a Herbal Product.

PURPOSE: Albendazole is the drug of choice for chemotherapy of cystic echinococcosis (CE) but 40% of patients do not respond to it satisfactorily. With regard to the previously reported scolicidal activity of Zataria multiflora, this study was done to evaluate the efficacy of the co-administration of albendazole and Z. multiflora aromatic water (AW) on hydatid cysts in a murine model. MATERIALS AND METHODS: Laboratory mice were infected with 1,500 viable protoscoleces. After development of hydatid cysts, the infected mice were allocated into four treatment and one control group. The mice of groups 1, 2, 3, and 4 were treated orally with albendazole (100 mg/kg), Z. multiform AW (100 ml/l), albendazole (100 mg/kg) - Z. multiform AW and albendazole ((50 mg/kg) - Z. multiform AW respectively. Two months later, all the mice were euthanized and necropsied and the number, size and weight of hydatid cysts were compared between different groups. RESULTS: The size of the largest cysts, as well as the total weight of cysts, were significantly lower (p < .05) in the mice treated with albendazole (50 mg/kg) - Z. multiflora AW in comparison to those treated with albendazole (100 mg/kg) alone. CONCLUSIONS: The results of this study revealed the superiority of co-administration of albendazole and Z. multiflora AW to albendazole alone in the treatment of CE in laboratory mice. Since Z. multiflora AW is a safe and useful drink, it may be used successfully to enhance the therapeutic effect of albendazole in the patients infected with hydatid disease.

In vitro and in vivo antihydatid activity of a nano emulsion of Zataria multiflora essential oil.

In in vitro process of this study, protoscoleces of the hydatid cysts were exposed to two concentrations of nano emulsion (NE) of Zataria multiflora essential oil (ZMEO) (1 and 2mg/mL) for 10 and 20min. Viability of protoscoleces was confirmed using 0.1% eosin staining. For in vivo studies, sixteen laboratory mice were infected intraperitoneally by 1500 live protoscoleces. Five months after infection, the infected mice were divided into treatment and control groups. The mice of treatment group received the NE of ZMEO (20mg/kg) orally via their drinking water while the mice of control group received no treatment. Two months after the start of treatment, all of the mice were necropsied and the hydatid cysts were collected. Subsequently, the numbers, sizes and weights of the collected cysts were compared between the mice of two groups. The results of in vitro scolicidal assays showed that the scolicidal power of NE of a ZMEO at concentration of 1mg/mL was 88.01%, and 100% after 10 and 20min respectively. NE of ZMEO showed 100% scolicidal power at a concentration of 2mg/mL after 10min (comparing to 4.46% for the control group). The results of in vivo studies revealed that the size of the largest cysts as well as the total number of the cysts were significantly lower in the mice treated with NE of ZMEO (P<0.05). In conclusion, NE of ZMEO may be considered as a natural scolicidal agent and a potential therapeutic tool for treatment of hydatid disease.