ABSTRACT
The present study investigated the lipid profile (fatty acid profile, positional distribution of n-3 fatty acids and phospholipid content) of head and skin of three lean fishes (gurnard, ribaldo and snapper). Gurnard head (GH) and snapper head (SnH) were found to contain a higher amount of total lipid (5.9-6.3%) than other samples (1.2-3.9%) including a considerable amount of bioactive n-3 fatty acids such as EPA (GH = 9.05%; SnH = 5.06%), DPA (GH = 2.78% ; SnH = 2.93%) and DHA (GH = 12.8% ; SnH = 7.72%) in the polar lipid fraction. DHA was found to predominate in the sn-2 position for gurnard head and snapper head. Partial least squares discriminant analysis showed that both gurnard and snapper samples were positively correlated with the n-3 fatty acids (EPA, DPA and DHA). Gurnard and snapper head had higher phospholipid content than the skin. Therefore, among the studied samples, GH and SnH are the best source of bioactive n-3 phospholipids.
Subject(s)
Lipidomics , Phospholipids , Animals , Chromatography, Gas , Docosahexaenoic Acids , Eicosapentaenoic Acid , Fatty Acids , Fishes , Magnetic Resonance SpectroscopyABSTRACT
This study used a novel extraction method (ETHEX) to extract the lipid content of King salmon head, skin and roe, and determined the lipid profiles using GC-FID, 13C NMR and 31P NMR spectroscopy. On a wet tissue basis, King salmon roe was found to contain the highest amount of phospholipid (26.53 µmol/g) and n-3 fatty acids (43.32%), followed by head (PL = 10.76 µmol/g; n-3 = 7.21%) and skin (PL = 4.98 µmol/g; n-3 = 8.23%). Total EPA (6.62%) and DHA (28.83%) content, along with the sn-2 positioned EPA (3.25%), DPA (1.36%) and DHA (16.35%) were also higher in roe compared with head and skin. The highest amount of EPA (7.99%) and DHA (34.47%) contents were found in the polar lipid fractions of roe, followed by skin (EPA = 4.19%; DHA = 25.95%) and head (EPA = 2.61%; DHA = 17.85%). This result suggests that salmon roe could be used for developing n-3 phospholipid enriched products.
