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Ann N Y Acad Sci ; 1108: 203-17, 2007 Jun.
Article in English | MEDLINE | ID: mdl-17893987

ABSTRACT

A novel method for isolation and purification of anti-ssDNA antibodies from human sera is developed. The process involves: antibody purification based on their affinity for single-stranded sequence of thymidines and removal of remaining components via protein G coated magnetic beads, with high affinity for only IgG subclass. The high degree of purity and molecular weights of healthy versus lupus anti-ssDNA antibodies were confirmed by SDS-PAGE and silver staining. Western blot confirmed IgG isotype. This novel technique allows for diagnostic purposes, structural and functional analysis of anti-DNA antibodies, and studies of their role in autoimmune diseases.


Subject(s)
Antibodies, Antinuclear/isolation & purification , Immunomagnetic Separation/methods , Lupus Erythematosus, Systemic/diagnosis , Oligodeoxyribonucleotides , Antibody Affinity , Blotting, Western , Electrophoresis, Polyacrylamide Gel , Humans , Sensitivity and Specificity
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