[Antioxidant properties and membranotropic effect of certain derivatives of 1,4-dihydropyridine]. / antiokislitel'nye svoistva i membranotropnoe deistvie nekotorykh proizvodnykh 1,4-digidropiridina.

Interaction of alpha-tocopherol and 1,4-dihydropyridine with endoplasmic reticulum membranes and model systems, human serum albumin and phospholipid bilayer, was studied using the microcalorimetry and fluorescent probes procedures. Dependence of microviscosity changes in the endoplasmic reticulum membranes on the place of antioxidants localization (protein structures or phospholipid phase) was shown. Increase of membrane structuralization under the influence of 1,4-dihydropyridines blocked their antioxidant action in spontaneous and induced lipid peroxidation.

[Antiradical and antioxidant properties of nonsteroidal anti-inflammatory agents--derivatives of pyridinecarboxylic acid]. / Antiradikal'nye i antiokislitel'nye svoistva nestroidnykh protivovospalitel'nykh sredstv--proizvodnykh piridinkarbonovykh kislot.

A number of pyridincarboxylic acid derivatives PV-1-4, 7 and emoxypine preparation antioxidative activity in yolk lipoprotein suspension was studied by a method of Fe(2+)-initiated biochemiluminescence. Lipid peroxidation in suspension was effectively inhibited by the studied compounds in various concentration ranges. PV 1, 3, 4, 7 inhibited lipid peroxidation at the concentrations 100-fold, then those of PV 2 and emoxypine. Antiradical activity of the studied compounds was demonstrated by their forming a complex with the stable diphenylpickrylhydrazyl radical. The effect of these compounds as antioxidants is discussed.

[Antiradical activity and membranotropic action of cardio- and vasotropic compounds of varying chemical nature]. / Antiradikal'naia aktivnost; i membranotropnoe deistvie kardio- i vazotropnykh soedinenii razlichnoi khimicheskoi prirody.

Antiradical activity and ability to interaction with phosphatidylcholine bilayer of the physiological active compounds (PhAC), concerning the classes of phenylalkilamines (dobutamine, verapamil), dihydro pyridines (BAY-K-4688, nifedipine), analogues of crown ether (carbicyle, diol) was studied. By means of the method of microcalorimetry and spectrophotometry it was shown the complexing ability PhAC with the phospholipid bilayer of the model membrane. It was stated the simbasity in the changes of the thermal effect of the compounds with the negative inotropic activity during its reaction with phosphatidylcholine bilayer and antiradical activity. That show on the presence of antiradical component in the mechanism that compounds' action.

[Interaction of antioxidants with differing chemical structure with a phospholipid bilayer]. / Vzaimodeistvie antioksidantov razlichnoi khimicheskoi struktury s fosfolipidnym bisloem.

Interaction of antioxidant (derivatives of phenol and 1,4-dihydropyridine) with bilayer from lecithin was studied by means of electronic spectroscopy and microcalorimetry. The connection of warm effects and spectral properties was estimated by chemical structure of antioxidants. The effect of physiologically active compounds on the structural and dynamic parameters of the bilayer was determined by means of fluorescent probing.

[Mechanisms of the genoprotective action of a phytoecdysteroid drug (BTK-8L) in chromatin damage by chlorofos]. / Mekhanizmy genoprotektornogo deistviia preparata na osnove fitoékdisteroidov (BTK-8L) v usloviiakh povrezhdeniia khromatina khlorofosom.

Genoprotective effect of preparation BTK-8L from plant steroids at prophylactic injection to experimental animals at chromatin damage by chlorofos was revealed. Antioxidant action of the preparation, being most highly expressed in the transcriptionally active chromatin fraction, is, probably, the most likely mechanism of the action. The realization of this action might be carried out as a result of the direct binding of BTK-8L with the chromatin protein-lipid complex, which was revealed during the analysis of the model systems showing binding of the preparation with chromatin fractions in vitro. Correction of structural chromatin organization and its transcriptional activity modified with intoxication of animals by chlorofos may occur as a result of such binding. Genoprotective properties in BTK-8L correlate to some extent with the lowering of the death rate of experimental animals at the early stage of intoxication by chlorofos at the prophylactic injection of the preparation, which, nevertheless, was not so significant, as in the case of intoxication by tetrachloromethane. Such fact allowed suggesting the existence of delayed unfavourable consequences for the organism (mutagenesis, cancerogenesis) as a result of the realization of the genotoxic action of chlorofos.

[Mechanisms of genoprotective action of a phytoecdysteroid drug(BTK-8L) in chromatin damage by tetrachloromethane]. / Mekhanizmy genoprotektornogo deistviia preparata na osnove fitoékdisteroidov (BTK-8L) v usloviiakh povrezhdeniia khromatina tetrakhormetanom.

Hepatoprotective action of prophylactic injection of aqueous solution of preparation BTK-8L from plant ecdysteroids to experimental animals with the liver damage by tetrachloromethane was revealed. This effect at least partially was connected with the genoprotective action of the given preparation. As a result, normalization of free radical chromatin lipid peroxidation reaction, modified at the intoxication, as well as partial correction of physical and chemical properties of chromatin protein-lipid complex were those molecular mechanisms of genoprotective action of BTK-8L, which were manifested by the influence of the preparation on such indices which characterized the depth structure of the complex as microviscosity and energy transfer from the protein to the lipid probe. Investigation of the interaction of the preparation with chromatin fractions in vitro and comparison of this interaction with the analogous process in model systems allowed revealing determinative participation of chromatin proteins and lipids in the given process. The preparation interacted more intensively with the active chromatin fraction, which contained a more marked protein-lipid complex, as comparing to the repressed one. Injection of the preparation also normalized such indices as relation between the chromatine fractions and protein/DNA ratio in them. On the contrary, injection of the alcoholic solution of the preparation to experimental animals, aggravated genotoxic tetrachloromethane action.

[Effect of the degree of liposome oxidation on adsorption by montmorillonite]. / Vliianie stepeni okisleniia liposom na ikh adsorbtsiiu montmorillonitom.

The influence of the degree of liposomes oxidation on the parameters of their adsorption by montmorillonite was studied. It was shown that accumulation of the products of oxidation in liposomes, which occurred in the process of their incubation, feebly influenced the ability of liposomes to adsorb on the surface of the clay mineral. The increase of the degree of liposomes oxidation in this case lead to the lowering of the values of adsorption heats, however the kinetics of heat production was preserved.

[Membranotropic action of cardiotonic agents and ubiquinone]. / Membranotropnoe deistvie kardiotonicheskikh sredstv i ubikhinona.

Interaction of cardiotonic drugs (strophantidine acetate, suphan, para-oxybenzoic acid) and ubiquinone with phospholipid bilayers has been studied. Exothermic effect of the reaction followed by an increase in microviscosity and hydrophobicity of the bilayer from cardiolipin, but by a decrease of the microviscosity of the bilayer from lecithin has been estimated. A correlation is observed between changes in the lecithin bilayer fluidity and the heat effect of the interaction at the initial period of time after mixing of reagents.