T cell adhesion to P-selectin induces tyrosine phosphorylation of pp125 focal adhesion kinase and other substrates.

Lymphocyte binding to endothelial surface adhesion molecules is an important early step in inflammation, which is mediated initially by P-selectin and E-selectin. We tested the hypothesis that lymphocyte binding to the selectin adhesion molecules induces intracellular signaling by tyrosine phosphorylation. We used an adhesion assay, which relied on cell binding to chimeric proteins consisting of the extracellular domains for P-selectin and E-selectin. Tyrosine phosphorylation was determined using anti-phosphotyrosine Abs by confocal microscopy and Western blot. Binding to P-selectin induced a significant increase in anti-phosphotyrosine immunoreactivity. The P-selectin effect was time dependent with an early response after 10 min and a maximum effect at 30 min. Western blot showed a time-dependent phosphorylation of two distinct 68- and 125-kDa proteins. These proteins were pp125 focal adhesion kinase (FAK) and paxillin, as shown by immunoprecipitation and colocalization. Phosphorylation of pp125 FAK was time dependent reaching a maximum after 30 min. Incubation with the tyrosine kinase inhibitor genistein, and, to a lesser extent, with the protein kinase C inhibitor staurosporine, resulted in decreased pp125 FAK phosphorylation. Our results are the first to demonstrate that lymphocyte binding to P-selectin induces tyrosine phosphorylation of distinct proteins. Thus, lymphocyte activation may occur already at the initial contact with surface adhesion molecules.

[Surgical management of vesico-renal reflux in adults]. / Operative Behandlung des vesikorenalen Refluxes im Erwachsenenalter.

27 units of ureterorenal reflux in adults were operated and analyzed between 1976 and 1980. It was shown that adequate preservation of function in refluxive kidneys with scarring grades I and II can be achieved by means of ureteroneocystostomy after the method of Cohen. The early removal of unilateral refluxive kidneys with high grade scarring is imperative. In cases of bilateral occurrence the indication for operative treatment should depend on the function demonstrated by isotopic investigations.

[Mitigated neurogenic bladder (author's transl)]. / Mitigierte neurogene blase.

The mitigated neurogenic bladder is gaining increasing significance in the investigation of children suffering from recurrent therapy-resistant infection of the urinary tract or enuresis. It is found in 33 to 48% of these cases and refers to isolated functional disorders of the bladder, which do not correspond to any neurological pattern. Morphological changes, if found, only occur as a result of functional changes. The functional correlations, diagnosis and therapy are discussed together with the description of 3 cases.