1.
J Chromatogr A
; 890(1): 159-66, 2000 Aug 18.
Article
in English
| MEDLINE
| ID: mdl-10976803
ABSTRACT
Magnetite and silica-magnetite composites were used as adsorbents for the isolation of genomic DNA from maize kernels. Two methods are described for the preparation of silica-magnetite composites, both of which afford higher yields of genomic DNA than when using magnetite alone, or a commerically available kit. DNA isolated using silica-magnetite was suitable for use in further applications such as polymerase chain reaction amplification and enzyme digestion.
Subject(s)
DNA, Plant/isolation & purification , Electrophoresis, Agar Gel/methods , Iron/chemistry , Oxides/chemistry , Silicon Dioxide/chemistry , DNA Restriction Enzymes/metabolism , DNA, Plant/analysis , DNA, Plant/metabolism , Ferrosoferric Oxide , Polymerase Chain Reaction , Zea mays/chemistry
2.
Anal Biochem
; 262(1): 92-4, 1998 Aug 15.
Article
in English
| MEDLINE
| ID: mdl-9735156