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ABSTRACT

Brugia malayi is a parasitic nematode that causes lymphatic filariasis in humans. Here the solution structure of the forkhead DNA binding domain of Brugia malayi DAF-16a, a putative ortholog of Caenorhabditis elegans DAF-16, is reported. It is believed to be the first structure of a forkhead or winged helix domain from an invertebrate. C. elegans DAF-16 is involved in the insulin/IGF-I signaling pathway and helps control metabolism, longevity, and development. Conservation of sequence and structure with human FOXO proteins suggests that B. malayi DAF-16a is a member of the FOXO family of forkhead proteins.

Subject(s)

Brugia malayi/metabolism , DNA/chemistry , Forkhead Transcription Factors/chemistry , Helminth Proteins/chemistry , Models, Molecular , Peptide Fragments/chemistry , Amino Acid Sequence , Animals , Binding Sites , Caenorhabditis elegans Proteins/chemistry , Caenorhabditis elegans Proteins/metabolism , Conserved Sequence , DNA/metabolism , Electrophoretic Mobility Shift Assay , Forkhead Transcription Factors/genetics , Forkhead Transcription Factors/metabolism , Helminth Proteins/genetics , Helminth Proteins/metabolism , Molecular Sequence Data , Molecular Weight , Peptide Fragments/genetics , Peptide Fragments/metabolism , Protein Conformation , Protein Structure, Tertiary , Recombinant Proteins/chemistry , Recombinant Proteins/metabolism , Response Elements , Sequence Alignment , Solubility

ABSTRACT

Subject(s)

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