ABSTRACT
PURPOSE: Gastric cancer is an aggressive disease which is the fourth prevalent malignancy in the world. Beside the genetic factors, epigenetic alterations such as promoter CpG island hyper methylation are involved in the emergence of gastric cancer. Herein, we investigated the methylation status of CDH11, EphA5, and HS3ST2 genes in patients with and without gastric adenocarcinoma for the first time. METHODS: In the study 40 paraffin-embedded tissue sections from gastric adenocarcinoma patients and 40 specimens from patients with functional dyspepsia were taken. DNA extraction was performed using a modified salting out method. Epizen DNA methylation kit was used to the bisulfite DNA conversion. The methylation status of CDH11, EphA5, and HS3ST2 genes were analyzed by methylation-specific PCR (MSP) technique. RESULTS: Among the 80 specimens, 71 DNA samples were achieved (34 gastric adenocarcinoma patients and 37 control patients). The results showed that CDH11, EphA5, and HS3ST2 genes are methylated in 28 (82.45%), 19 (55.88%), and 26 (76.47%) of 34 DNA samples from gastric adenocarcinoma patients, respectively, whereas, these genes are methylated in 7 (18.91%), 9 (24.32%) and 7 (18.91%) of 37 samples from noncancerous patients, respectively. Statistical analyses using a chi-squared test showed that there is a statistically significant difference in methylation level of CDH11, EphA5, and HS3ST2 genes between gastric cancer and uncancerous patients (p < 0.05). CONCLUSION: To the best of our knowledge, this is the first report on methylation of CDH11, EphA5, and HS3ST2 promoters' in gastric adenocarcinoma patients using MSP. Identification of novel cancer-related molecular mechanisms can be useful in detection of new treatment strategies.
Subject(s)
Adenocarcinoma/genetics , Cadherins/genetics , CpG Islands , DNA Methylation , Receptor, EphA5/genetics , Stomach Neoplasms/genetics , Sulfotransferases/genetics , Adenocarcinoma/metabolism , Adenocarcinoma/pathology , Aged , Aged, 80 and over , Biomarkers, Tumor , Cadherins/metabolism , Case-Control Studies , DNA, Neoplasm/genetics , DNA, Neoplasm/metabolism , Female , Humans , Male , Middle Aged , Promoter Regions, Genetic , Receptor, EphA5/metabolism , Stomach Neoplasms/metabolism , Stomach Neoplasms/pathology , Sulfotransferases/metabolismABSTRACT
Fetus-in-fetu is a rare congenital condition in which a malformed fetus-like structure is found in the body of its twin. We report a unique case of a male neonate with cleft palate and a fetus-like structure arising in his oral cavity. The neonate underwent emergent surgical removal of the mass immediately after delivery. Radiological and pathological studies of the resected mass provided supportive evidence for the case of an oral fetus-in-fetu. To our knowledge, there are few cases of oral fetus-in-fetu in the literature. Moreover, the presence of cleft palate in this neonate is of potential interest and clinical importance.
