ABSTRACT
Application of Mössbauer spectroscopy with a high velocity resolution (4096 channels) for comparative analysis of iron cores in a human liver ferritin and its pharmaceutically important models Imferon, Maltofer(®) and Ferrum Lek as well as in iron storage proteins in chicken liver and spleen tissues allowed to reveal small variations in the (57)Fe hyperfine parameters related to differences in the iron core structure. Moreover, it was shown that the best fit of Mössbauer spectra of these samples required different number of components. The latter may indicate that the real iron core structure is more complex than that following from a simple core-shell model. The effect of different living conditions and age on the iron core in chicken liver was also considered.
Subject(s)
Ferritins/analysis , Ferritins/chemistry , Iron-Dextran Complex/chemistry , Iron/analysis , Liver/metabolism , Organometallic Compounds/chemistry , Polysaccharides/chemistry , Spectroscopy, Mossbauer/methods , Spleen/metabolism , Animals , Chickens , Freeze Drying , Humans , Iron/chemistry , Iron-Dextran Complex/analysis , Models, Chemical , Organometallic Compounds/analysis , Polysaccharides/analysis , TemperatureABSTRACT
A hard phase immunoenzymatic system for primary screening and subsequent quantitative determination of autoimmunobodies to the surface of islet cells (ASIC) was worked out using rat target cells. ASIC organ-specificity was confirmed by the absence of crossreactions with rat hepatocytes and splenocytes. The determination of the upper normal bound with 99% confidence interval combined with a high sensitivity of the method allowed an objective and significant assertainment of ASIC-positive sera even with a low level of antibodies. ASIC quantitative characteristic is their titer determined with the help of the intersection of a titration curve with the upper normal bound. ASIC were detected approximately in half of the patients with insulin-dependent diabetes of a duration of maximum 5 years and were not detected in the patients with insulin-dependent diabetes compensated with diet and sugar lowering drugs using the above method.
