[Development of new technology for isolation of nebramycin complex antibiotics from the cultural fluid]. / Razrabotka novoi tekhnologii vydeleniia antibiotikov nebramitsinovogo kompleksa iz kurtural'noi zhidkosti.

A new technology for nebramycin complex antibiotics isolation from non-filtered culture fluid was developed. The industrial use of the technology permitted to exclude the stage of the culture fluid filtration, to increase the yield by more than 33% and to reduce the time of the process by 1.7 times.

[A novel laboratory method of isolation and purification of tobramycin]. / Razrabotka novogo laboratornogo metoda vydeleniia i ochistki tobramitsina.

A new laboratory method for isolation and purification of tobramycin by using extraction of a tobramycin derivative with benzaldehyde by methylene chloride, subsequent hydrolysis of azomethine and recrystallization of the formed tobramycin sulfate from solution of sulfuric acid in methanol was developed. The method allows to exclude the stage of chromatographic purification of tobramycin, to reduce the time of the process realization from 120-125 h to 15-20 h, to increase the yield of the target product from 37-40% to 60-65% without decreasing the product quality, to exclude a number of large-size and expensive equipment and to ensure high reproducibility of the technology.

[Development of technology for isolation and purification of gentamicin using sorption without filtration]. / Razrabotka tekhnologii vydeleniia i ochistki gentamitsina s ispol'zovaniem metoda besfil'tratsionnoi sorbtsii.

A technology for isolation and purification of gentamicin from nonfiltered fermentation broth with the use of sorption-pulsory columns was developed as a result of the research work. The practical application of the technology will increase the yield from 30 to 57 per cent and reduce the content of the minor admixtures in the final product up to 5 per cent in comparison to the currently used process.

[The structural-functional changes in the cells of Francisella tularensis strain 15 Gaiskii during cultivation and sublimational drying]. / Strukturno-funktsional'nye izmeneniia kletok Francisella tularensis shtamma 15 Gaiskogo pri kul'tivirovanii i sublimatsionnom vysushivanii.

Instrumental methods of investigation were used for the demonstration of changes in the fatty acid composition of F. tularensis, strain 15 Gaiskii, during cultivation in solid culture medium, storage after lyophilization, as well as changes in the functioning of the system of membrane-dependent enzymes of the respiratory chain and in the permeability of cell wall membranes by water molecules and NADH after lyophilization. A relationship between the survival rate of F. tularensis cells after lyophilization and stimulation of their endogenic respiration with NADH and succinate was revealed. An increase in residual moisture from 6 to 10-12% was found to intensify the process of lipid peroxidation during the storage of lyophilized F. tularensis cells of strain 15 Gaiskii.