ABSTRACT
AIM: To create individual learning curves for microsurgical myocardial revascularization. MATERIAL AND METHODS: It was analyzed duration of the individual stages of the first 100 CABG procedures which were performed using microsurgical technique and surgical microscope. Graphs reflecting correlation between duration of the individual surgical stages and their number were framed. RESULTS: Improvement in distal anastomosis time, aortic cross-clamping time, cardiopulmonary bypass time and duration of surgery was observed with increased surgeon's experience. CONCLUSION: Individual learning curves objectively characterize the learning process of microsurgical myocardial revascularization, allow to estimate the time need for improvement of cardiac surgeons' qualification and demonstrate dates for achieving average surgical quality during learning the technique.
Subject(s)
Coronary Artery Bypass/methods , Coronary Artery Bypass/statistics & numerical data , Learning Curve , Microsurgery/statistics & numerical data , Clinical Competence , Coronary Artery Bypass/instrumentation , Humans , Microsurgery/instrumentation , Microsurgery/methods , Time FactorsSubject(s)
Antineoplastic Agents/pharmacology , Melanoma, Experimental/drug therapy , Soybean Proteins/pharmacology , Animals , Antineoplastic Agents/administration & dosage , Cyclophosphamide/pharmacology , Female , Mice , Mice, Inbred C57BL , Soybean Proteins/administration & dosage , Tegafur/pharmacologyABSTRACT
The Argiope lobata venom is shown to block synaptic potential at locust neuromuscular junctions and inhibit the high-affinity sodium independent L[3H]glutamate binding site in locust muscle membranes. The data obtained due to fractionation of venom evidence that it contains components which block synaptic potential and inhibit the binding of L[3H]glutamate (5 kDa and more) as well as components which block synaptic potential but do not inhibit the binding of L[3H]glutamate less than 5 kDa. These observations indicate that spider venom contains at least two components with different mechanism of action.
Subject(s)
Arthropod Venoms/pharmacology , Glutamates/metabolism , Neuromuscular Junction/metabolism , Spider Venoms/pharmacology , Animals , Binding, Competitive , Glutamic Acid , Grasshoppers , In Vitro Techniques , Molecular Weight , Neuromuscular Junction/drug effects , Spider Venoms/isolation & purificationABSTRACT
Two neurotoxins and one insectotoxin have been isolated from venom of the cellar spider Segestria florentina, their homogeneity being proved by disk electrophoresis, isoelectric focusing, and analysis of N-terminal amino acid residues. The neurotoxins are polypeptides with molecular mass about 5000 D. For the insectotoxin, containing 35 amino acid residues with molecular mass 3988 D, the total primary structure is established.
Subject(s)
Arthropod Venoms/analysis , Neurotoxins/analysis , Spider Venoms/analysis , Action Potentials/drug effects , Amino Acid Sequence , Amino Acids , Animals , Anura , Chromatography, Gel , Chymotrypsin , Hydrolysis , In Vitro Techniques , Neuromuscular Junction/drug effects , Neuromuscular Junction/physiology , Neurotoxins/pharmacology , Spider Venoms/pharmacologySubject(s)
Androstane-3,17-diol , Androstanols , Anesthesia, General/methods , Hip Dislocation, Congenital/surgery , Muscle Relaxants, Central , Piperazines , Adolescent , Androstane-3,17-diol/analogs & derivatives , Child , Child, Preschool , Drug Evaluation , Humans , Intraoperative Care , Pipecuronium , Preanesthetic MedicationABSTRACT
It has been shown that tetrodotoxin inhibits GABA-receptor binding of muscimol with solubilized membranes of the rat brain in a competitive manner, with the inhibition constant amounting to about 3 nM. Muscimol has been also demonstrated to competitively inhibit 3H-tetrodotoxin binding with the same drug.
