Potential Adsorption Affinity of Estrogens on LDPE and PET Microplastics Exposed to Wastewater Treatment Plant Effluents.

Microplastics (MPs) are among the most common pollutants in the environment. Because of their small size, availability, and similarity to natural foods, they are commonly ingested by marine organisms. They can cause health problems in living organisms due to their bioaccumulation potential. It is, therefore, unknown whether endocrine-disrupting chemicals (EDCs), in particular estrogens, are capable of adhering to the diverse types of MPs found in water. Two MP polymers (low-density polyethylene (LDPE) and polyethene terephthalate (PET)) that could pose a threat to fish were tested for estrogen adsorption. The adsorption capacity of MP pellets was studied for 30 days in the effluent and influent of a wastewater treatment-plant. A laboratory simulation was conducted to validate the field and laboratory findings. We found that the concentrations of five types of estrogen ((diethylstilbestrol (DES), estrone (E1), 17ß-estradiol (E2), estriol (E3), 17α-ethinyl estradiol (EE2)) were higher in the influent than the effluent streams. LDPE and PET MPs exposed to influent water in the laboratory had higher estrogen levels than wastewater treatment plants (WWTP) pellets. The PET pellets showed the highest adsorption affinity to EE2, while the LDPE pellets showed the highest affinity to E2. As a result, this study provided baseline data to investigate the estrogen adsorption capacity in MPs.

Petroleum hydrocarbon pollution in sediments from the Gulf and Omani waters: Status and review.

This review presents the spatio-temporal distribution of petroleum hydrocarbons including total petroleum hydrocarbon (TPH), total organic carbon (TOC), total aliphatics, unresolved complex mixture (UCM), polycyclic aromatic hydrocarbons (PAHs), and total aromatic hydrocarbons in marine sediments of the Gulf (Iraq, Iran, Kuwait, Saudi Arabia, Bahrain, Qatar, United Arab Emirates and Oman). The TPH ranged between 0.134 and 48,018 µg g-1 dw where 10-15 µg g-1 dw was considered as a background concentration. The TOC levels were between 0.04 and 14.96% with a mean concentration of 1.154 ±â€¯0.523%. Total aliphatic hydrocarbon levels were reported between 0.1 and 76 µg g-1, the 2005 levels that had the largest spatial coverage were between 0.1 and 4.4 µg g-1. The unresolved complex mixture was very variable post Gulf War but by 2005 most of the Gulf War artifacts had decreased and the levels were between 1.5 and 73.5 µg g-1. The Æ©PAHs in bottom sediments by 2005 were between 0.3 and 3450 ng g-1. The total aromatics were limited in spatial extent and varied between 1.0 and 14,000 µg g-1. Most of the locations with elevated contamination levels were near point sources, e.g. oil facilities and ports, and these sites could be categorized as chronically contaminated by oil. This review highlights the paucity of the data both in terms of the spatial extent and temporal coverage, and with several Gulf states undergoing large-scale coastal developments and offshore oil exploration, it will be prudent to undertake regular monitoring of the petroleum hydrocarbons to ensure effective ecosystem functioning as well as seafood and drinking water safety in the Gulf region. The spatial distribution also highlights the lack of uniformity in assessments and the need to support marine pollution assessments in the Gulf countries.

Standardized protocols for microplastics determinations in environmental samples from the Gulf and marginal seas.

Microplastics are a group of ubiquitous persistent pollutants that have rapidly attracted much attention from the scientific community as well as the general public due to the growing awareness of the environmental risks they pose. However, due to limitations and variations in sampling, analytical measurement methods, and the different units used for reporting data, reliable comparisons between studies in the Gulf region and internationally are not straightforward. This study proposes standardized protocols for marine sediment, seawater, marine biota and aerosol (1) sampling, (2) sample processing, (3) sample identification and (4) reporting units to be used. An attempt has been made to highlight the limitations of the widely employed strategies for sampling microplastics in seawater, where a large portion of the microplastics is not sampled due to the mesh sizes used. The issues with the processing of biota samples and aerosols are likewise addressed, and recommendations are also made for standardization of units for reporting microplastic quantification. Protocols for collection of bottom sediments and aerosols are also proposed. These are the environmental matrixes for which there are no harmonized protocols in the Gulf region; hence if a standardized approach is adopted, it will enable and improve comparisons between the studies within this region and can be useful for similar studies in other marine areas as well.

Microplastic particles in the Persian/Arabian Gulf - A review on sampling and identification.

Microplastics are ubiquitous, persistent pollutants that are reported in abundance within the marine environment. Their presence in seawater and marine sediments poses a legitimate environmental and ecological concern for toxicity and food chain transfer via marine organisms. Their capability for sorption of other hydrophobic contaminants and the inability of the wastewater treatment plants to completely remove them pose additional risks. This review highlights the methodologies for sampling, sample preparation, and identification used in the Persian/Arabian Gulf region, which is possibly one of the least studied marginal seas with only sixteen papers published on microplastics. The review highlights the several orders of magnitude variations in microplastic concentrations among different studies; e.g. in seawater, only 12 microplastic particles were reported from 40 transects of one km length in Kuwait to 0.71 microplastics m-3 in Qatar. Concentrations in beach sediments also show the significant difference between the northern and southern Gulf coasts, with 13 particles in 24 samples reported in Qatar, and 15 particles within 44 samples across Kuwait, to 3252 ± 2766 particles m-2 from Bandar Abbas, Iran. The biota samples also show similar variances, with only three particles identified from 87 gut samples in Kuwait to 828 particles in 58 samples that include 46 fish and 12 shrimps from Iran. Some extremely high concentrations in biota are also reported from Iran, with concentrations as high as 0.251 particles g-1 of muscle and 0.931 particles g-1 in gills. It is evident that there is no consensus in the Gulf region on the sampling techniques (mesh size of plankton nets and sieves), use of fluidization solutions and very different units used in data reporting such as particles m-3 and particles m-2 in water samples. In sediments units like particles g-1 and particles m-2 have been used, and for biota it is the number of particles present in the sample, while others have quantified data as particles g-1 of tissue. Considering the higher densities of PET, PVC, nylon and polyester than seawater, they are likely to migrate downwards into marine sediments, a transfer process that has not been studied in detail. Thus the review underscores the need to adopt harmonized protocols for microplastic studies in the region, and identifies certain aspects of microplastics that require further study.

Microplastics in Kuwait marine environment: Results of first survey.

Microplastic pollution status in Kuwait coastal areas was assessed A total of 44 intertidal locations were sampled for beach sediment. Short trawls (40) were conducted. In addition, 87 fish and mussels gastrointestinal contents were examined. Microplastics were characterized by Raman spectroscopy. Contrary to the expectation very few microplastic particles were found. Only 37 MPs were detected in beach sediments at 15 locations. Seawater trawls indicated that MPs were low in the numbers. MPs were found in just two samples from Kuwait Bay and also in two samples from the southern areas. In biota, only 3 pieces of plastics were recovered from gastrointestinal tracks of hamour fish. The identified MPs were dominantly polypropylene, polyethylene and polystyrene. It appeared that the microplastic levels in sediment, water and biota were much lower compared to published values from adjoining areas, however, were comparable to the absolute numbers of particles from Qatar and Oman.

Impact of endocrine disrupting compounds in sewage impacted coastal area on seabream.

The pollution of coastal regions worldwide has been of a great concern due to the presence of endocrine disrupting chemicals (EDCs). These chemicals find their way to the marine environment via the sewage treatment plants (STPs). Hence, this study was designed to investigate the status and sources of EDCs and their effect on fish in Kuwait's coastal areas, from the chemical and biological perspectives. The assessment of three STPs indicated the presence of significant levels of phthalates (19 and 31µg/l), alkylphenols (85 and 159ng/l), and estrogens (30 and 368ng/l) in both inflow and outflow samples. The analysis of samples from field exposure sites revealed significant levels of EDCs in seawater (phthalates: 2.1-4.6µg/l; alkylphenols: 1.2-16.4ng/l; estrogens: 0-36.2ng/l) and sediment (phthalates: 2.1-15.7mg/kg dry wt; alkyphenols: 2.5-15.1µg/kg dry wt.; estrogens: 4.1-214.2µg/kg dry wt.) samples. The biological perspective investigated through the exposure of fish to sewage outlets at five sites. The hepatosomatic index (HSI) revealed a higher level in winter samples 0.48-0.79%) in comparison to summer samples 1-1.5%). Histological observation of hepatic tissue of fish exposed during winter months in all sites, showed much less necrotic changes and hepatic vacuolation in the hepatic tissue of summer exposed fish. Imunnohistochemistry evidences revealed a significant level of positive signals and Vtg localization in the hepatic tissue as the results support the histopathological alterations observed. Results of enzyme-linked immunosorbent assay (ELISA) showed no significant difference between the plasma protein content of winter and summer samples. Overall, the study suggest that there is possible local source or a chronic input of untreated and/or partially treated water due to the significant levels of phthalates, alkyphenols, and estrogens detected in the Kuwait Bay. These levels were enough to initiate alteration in the hepatic tissue of fish exposed to the sewage outlets in Kuwait for two weeks.

Sources and levels of endocrine disrupting compounds (EDCs) in Kuwait's coastal areas.

The sources and levels of endocrine disrupting compounds in Kuwait's coastal areas were investigated. Phthalates, alkylphenols and estrogens were measured in the inflows and outflows of three sewage treatment plants as well as in the seawater and sediments from the sewage impacted coastal areas. Phthalate levels in the inflow of the treatment plants ranged from 8.9 to 78.3µg/l; alkylphenols from 0.7 to 279ng/l and estrogens from 30 to 368ng/l. On average, the treatment plants removed about 80% of these compounds. The outflows, however, contained significant levels of all three classes of compounds. The seawater from the sewage impacted area also contained detectable levels of these compounds. Sediment samples from these locations contained elevated levels of phthalates (ranging from 2145 to 15,722µg/kg) and lower levels of alkylphenols (ranging from 2.49 to 15.14µg/kg) and estrogens (ranging from 4.1 to 214µg/kg, dry wt.).

Spatial assessment of the sewage contamination of Kuwait's marine areas.

Discharge of sewage to the coastal areas resulting in the deteriorating quality of seawater and polluted sediments has been one of important stressor in Kuwait. The objective of this study was to conduct spatial assessment of sewage contamination of coastal areas. The assessment was carried out by measuring fecal sterols as indicator of sewage contamination, in the marine sediments collected from 112 locations throughout the Kuwait's marine areas. The samples were extracted and sterols separated. Derivatized sterols were analyzed by GC/MS in selected ion monitoring mode. The results showed that areas in the vicinity of the sewage outfalls were heavily contaminated. The western part of Kuwait Bay was worst in terms of contamination level. Two off-shore sites in Kuwait Bay were also classified as contaminated. Coprostanol levels in Kuwait Bay ranged from 0 to 39,428 ng/g. Southern coastal areas were less severely contaminated.

Effect of environmental factors on photodegradation of polycyclic aromatic hydrocarbons (PAHs) in the water-soluble fraction of Kuwait crude oil in seawater.

Photodegradation of PAHs in the water-soluble fraction of Kuwait crude oil in seawater was investigated under various environmental factors (temperature, light intensity, oxygen levels and presence of a sensitizer) in laboratory conditions. All factors investigated had significant effect on the degradation rates of PAHs. At 15 °C almost all PAHs optimally degraded at an oxygen level of 4 ppm. For lower molecular weight PAHs a light intensity of 500 W/m(2) in the presence of the sensitizer worked well. Higher molecular weight PAHs degraded at faster rates at a light intensity 750 W/m(2). At 30 °C, most of the PAHs degraded optimally at an oxygen level of 0 ppm and light intensity of 500 or 750 W/m(2) in presence of the sensitizer. At 40 °C, most of PAHs degraded optimally at low oxygen concentrations (0 and 4 ppm) and a light intensity of 500 W/m(2) in the presence of the sensitizer. Linear regression indicated that for most of the compounds, light intensity had the greatest effect on degradation rates.