ABSTRACT
We report the first detection of Senecavirus A (SVA) in nine of 12 (75%) pigs in Thailand in 2016. The full-length genome demonstrated that Thai SVA isolates were closely related to the first Canada strain (11-55910-3) than the recent strains causing outbreaks in Brazil, the United States and China in 2015-2016.
Subject(s)
Disease Outbreaks/veterinary , Picornaviridae Infections/veterinary , Picornaviridae/isolation & purification , Swine Diseases/virology , Animals , Genome, Viral/genetics , Phylogeny , Picornaviridae Infections/epidemiology , Picornaviridae Infections/virology , Swine , Swine Diseases/epidemiology , Thailand/epidemiologyABSTRACT
Porcine deltacoronavirus (PDCoV) was detected by RT-PCR in 12 of 97 (12.4%) intestinal samples collected during 2015 from piglets with diarrhoea in Thailand, Vietnam and Lao PDR. Spike, membrane and nucleocapsid genes were characterized, and phylogenetic analyses demonstrated that PDCoV isolates from Thai and Lao PDR form a novel cluster, separated from US and China isolates, but relatively were more closely related to China PDCoV than US isolates. Vietnam PDCoVs, however, were grouped together with US PDCoV. The analyses of amino acid changes suggested that they were from different lineage.
Subject(s)
Coronaviridae/genetics , DNA, Viral/genetics , Swine Diseases/virology , Animals , Coronaviridae Infections/genetics , Intestines/virology , Laos , Phylogeny , Polymerase Chain Reaction , Swine , Thailand , VietnamABSTRACT
In Thailand, five species of Ehrlichia (E. canis, E. chaffeensis, E. equi, E. risticii and Anaplasma platys) have been reported to infect dogs. Although ehrlichial infections can cause ocular disorders, the severity and type of ocular disorder varies between individual infected dogs. The aims of this study were to determine the factors associated with retinal detachment and to investigate the species of Ehrlichia that cause ocular disorders in natural infected dogs. In the present study, ocular examination, complete blood count and total protein measurement were performed in 134 dogs brought into an ophthalmology clinic. A 310 bp fragment of the Ehrlichia 16s rRNA gene was amplified by nested-PCR and direct DNA sequenced. Thirty-eight of these dogs were found to be positive for Ehrlichia 16s rRNA, of which the sequence analysis suggested 34 and 4 dogs were infected with E. canis and A. platys, respectively, with no multiple infections or other Ehrlichia species detected. The most common ocular disorders in dogs infected with E. canis were blindness, keratoconjunctivitis sicca and retinal detachment, while blindness and retinal detachment were found in A. platys-infected dogs. Hematological disorders were found anemia, thrombocytopenia and hyperproteinemia. Odd ratio analysis showed that thrombocytopenia and anemia were likely important factors for increasing retinal detachment risk. In this study, only E. canis and A. platys closely relate to be causative agents of ocular disorders in infected dogs. To the best of our knowledge, this is the first report of A. platys as a causative pathogen of both anterior and posterior uveitis in clinical situations.
