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1.
Methods Enzymol ; 587: 351-364, 2017.
Article in English | MEDLINE | ID: mdl-28253965

ABSTRACT

Autophagy (self-eating) is a common term for various processes by which cellular components are transferred to lysosomes for degradation. In macroautophagy, intracellular membrane structures termed "phagophores" expand to encapsulate autophagic cargo into sealed, double-membrane vacuoles termed "autophagosomes," which subsequently may fuse with endosomes to form intermediary vacuoles called "amphisomes," and finally with lysosomes to have their contents degraded and recycled. Autophagy is frequently analyzed by monitoring phagophore- and autophagosome-associated markers such as LC3. Although useful, it is becoming increasingly clear that very few, if any, of these marker proteins are entirely specific to the autophagic process. Moreover, phagophore/autophagosome markers cannot be used to measure autophagic activity since they are part of the autophagic machinery, or "cart," rather than autophagic cargo. Thus, there is a great need for functional assays in autophagy research. Here, we describe a method that quantitatively measures the nonselective autophagic sequestration of endogenous cytosolic cargo. The method is based on a crude separation of sedimentable cellular material from cytosol and a subsequent measurement of the fraction of a cytosolic enzyme activity transferred to the sedimentable fraction by autophagic sequestration. The original assay was first developed in 1990, but during the last few years we have systematically downscaled and simplified the method into the time- and cost-efficient procedure presented here, which can be performed with standard laboratory equipment and is suitable for any cell type.


Subject(s)
Autophagy , L-Lactate Dehydrogenase/metabolism , Molecular Biology/methods , Animals , Cell Membrane/chemistry , Cells, Cultured , Cytosol/metabolism , Rats
2.
Toxicon ; 39(2-3): 349-62, 2001.
Article in English | MEDLINE | ID: mdl-10978754

ABSTRACT

The diarrhetic shellfish toxin, okadaic acid, administered to rats by intragastric intubation, caused intestinal damage, diarrhea and death, but had no detectable effect on the liver. In contrast, okadaic acid administered intravenously had little effect on intestinal function, but caused a rapid dissolution of hepatic bile canalicular actin sheaths, congestion of blood in the liver, hypotension and death at high doses. In isolated rat hepatocytes, okadaic acid induced disruption of the canalicular sheaths as well as of the keratin intermediate filament network. Both of these cytoskeletal changes could be prevented by addition of a cytoprotective flavonoid, naringin, to the isolated hepatocytes, whereas intravenously or intragastrically administered naringin failed to protect against the effects of okadaic acid in vivo. Freshly isolated colonocytes already had fragmented keratin and tubulin cytoskeletons, died rapidly and were not further afflicted by okadaic acid. Naringin had no protective effect on isolated colonocytes or on intestinal function in vivo, but the nonspecific protein kinase inhibitor, K-252a, and the protein-tyrosine-phosphatase inhibitor, vanadate, significantly reduced the extent of colonocytic keratin fragmentation, and an inhibitor of apoptotic caspases, zVAD.fmk, was strongly protective. Further studies of hepatic and intestinal cytoprotectants should focus on conditions that limit their effectiveness in vivo.


Subject(s)
Antioxidants/therapeutic use , Carcinogens/antagonists & inhibitors , Carcinogens/toxicity , Flavanones , Flavonoids/therapeutic use , Intestines/drug effects , Liver/drug effects , Okadaic Acid/antagonists & inhibitors , Okadaic Acid/toxicity , Animals , Carcinogens/administration & dosage , Cell Survival/drug effects , Cytoskeleton/drug effects , DNA Fragmentation/drug effects , Injections, Intravenous , Intestines/pathology , Intubation, Gastrointestinal , Male , Okadaic Acid/administration & dosage , Rats , Rats, Wistar
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